Identification and characterization of host cell protein product-associated impurities in monoclonal antibody bioprocessing

Biotechnology and Bioengineering

Volumn 111, Issue 5, 2014, Pages 904-912

  Levy, Nicholas E ^a   Valente, Kristin N ^a   Choe, Leila H ^a   Lee, Kelvin H ^a   Lenhoff, Abraham M ^a  

^a UNIVERSITY OF DELAWARE   (United States)

Author keywords

Host cell protein impurities; MAb process development; Protein A affinity chromatography; Protein protein interactions

Indexed keywords

AFFINITY CHROMATOGRAPHY; ASSOCIATION REACTIONS; ELECTROPHORESIS; MASS SPECTROMETRY; MONOCLONAL ANTIBODIES; PRODUCT DESIGN; REMOVAL;

DOWNSTREAM-PROCESSING; HOST CELL PROTEIN; MONOCLONAL ANTIBODIES (MABS); PROCESS DEVELOPMENT; PROTEIN A; PROTEIN-PROTEIN INTERACTIONS; REMOVAL OF IMPURITIES; TWO-DIMENSIONAL GEL ELECTROPHORESIS;

PROTEINS;

CELL PROTEIN; HOST CELL PROTEIN; IMMUNOGLOBULIN F(AB) FRAGMENT; IMMUNOGLOBULIN FC FRAGMENT; MONOCLONAL ANTIBODY; PROTEIN A; UNCLASSIFIED DRUG;

AFFINITY CHROMATOGRAPHY; ANIMAL CELL; ARTICLE; CHROMATOGRAPHY; CROSS INTERACTION CHROMATOGRAPHY; DOWNSTREAM PROCESSING; FEMALE; HOST CELL; MASS SPECTROMETRY; NONHUMAN; PROTEIN INTERACTION; PROTEIN PURIFICATION; PROTEOMICS; TWO DIMENSIONAL GEL ELECTROPHORESIS;

HOST CELL PROTEIN IMPURITIES; MAB PROCESS DEVELOPMENT; PROTEIN A AFFINITY CHROMATOGRAPHY; PROTEIN-PROTEIN INTERACTIONS;

ANIMALS; ANTIBODIES, MONOCLONAL; CHO CELLS; CHROMATOGRAPHY, AFFINITY; CRICETINAE; CRICETULUS; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; PROTEINS; RECOMBINANT PROTEINS;

EID: 84897079355     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.25158     Document Type: Article

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