Effects of high passage cultivation on CHO cells: A global analysis

Applied Microbiology and Biotechnology

Volumn 94, Issue 3, 2012, Pages 659-671

  Beckmann, T F ^a   Kramer O ^a   Klausing, S ^a   Heinrich, C ^a   Thute T ^a   Buntemeyer H ^a   Hoffrogge, R ^a   Noll, T ^a  

^a BIELEFELD UNIVERSITY   (Germany)

Author keywords

Chinese hamster ovary (CHO) cells; Dihydrofolate reductase (DHFR) gene; Genetic stability; Long term cultivation; Omic techniques; Specific productivity

Indexed keywords

CHINESE HAMSTER OVARY CELLS; DIHYDROFOLATE REDUCTASE; GENETIC STABILITY; OMIC TECHNIQUES; SPECIFIC PRODUCTIVITY;

CELL CULTURE; CELLS; ENZYME ACTIVITY; FLOW CYTOMETRY; GENE EXPRESSION; MONOCLONAL ANTIBODIES; POLYMERASE CHAIN REACTION; PRODUCTIVITY; PROTEINS;

CULTIVATION;

ADENOSINE PHOSPHATE; CELL PROTEIN; MESSENGER RNA; METHOTREXATE; MONOCLONAL ANTIBODY;

ANTIBODY; CELL ORGANELLE; ENERGY EFFICIENCY; FLOW CYTOMETRY; GENETIC ANALYSIS; METABOLITE; POLYMERASE CHAIN REACTION; PROTEIN; PROTEOMICS;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; BIOREACTOR; CELL CULTURE; CELL DENSITY; CELL GROWTH; CELL SUBPOPULATION; CHO CELL; CRYOPRESERVATION; FLOW CYTOMETRY; GENE DOSAGE; GENE LOSS; GENOMICS; GLYCOLYSIS; HIGH PASSAGE CULTIVATION; NONHUMAN; NUTRIENT CONCENTRATION; PH; PHENOTYPE; PRODUCTIVITY; PROTEOMICS; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; RNA SPLICING; TWO DIMENSIONAL GEL ELECTROPHORESIS;

ADAPTATION, BIOLOGICAL; ANIMALS; ANTIBODIES, MONOCLONAL; CHO CELLS; CRICETINAE; CRICETULUS; RECOMBINANT PROTEINS; SERIAL PASSAGE;

CRICETULUS GRISEUS;

EID: 84862657777     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-011-3806-1     Document Type: Article

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