Profiling of host cell proteins by two-dimensional difference gel electrophoresis (2D-DIGE): Implications for downstream process development

Biotechnology and Bioengineering

Volumn 105, Issue 2, 2010, Pages 306-316

  Jin, Mi ^a   Szapiel, Nicolas ^a   Zhang, Jennifer ^a   Hickey, John ^a   Ghose, Sanchayita ^a  

^a BRISTOL MYERS SQUIBB COMPANY   (United States)

Author keywords

Downstream process development; Host cell proteins; Profiling; Two dimensional difference gel electrophoresis

Indexed keywords

2D DIGE; BIOLOGIC DRUGS; BIOREACTOR CONTROL; CELL CULTURE MEDIA; CELL VIABILITY; CHO CELL; COMPLEX MIXTURE; DOWNSTREAM PROCESS; FEEDING STRATEGIES; GEL ELECTROPHORESIS; HOST CELL PROTEIN; IMMUNOLOGICAL PROPERTIES; MOLECULAR PROPERTIES; PROCESS CONDITION; PRODUCTION CELLS; PROTEOMIC PROFILING; UPSTREAM PROCESS;

ANTIGENS; CELLS; ELECTROPHORESIS; GELS; HARVESTING; IMMUNOLOGY; MONOCLONAL ANTIBODIES; PROCESS DESIGN; PROTEINS; TWO DIMENSIONAL;

CELL CULTURE;

CELL PROTEIN;

ANIMAL CELL; ANIMAL CELL CULTURE; ARTICLE; CELL VIABILITY; CHO CELL; CONTROL STRATEGY; CONTROLLED STUDY; CULTURE MEDIUM; DOWNSTREAM PROCESSING; FEMALE; HOST CELL; IMMUNOASSAY; NONHUMAN; NULL CELL; PROTEIN ANALYSIS; PROTEIN SYNTHESIS; PROTEOMICS; REACTOR MONITORING; TWO DIMENSIONAL GEL ELECTROPHORESIS;

ANIMALS; BIOREACTORS; BIOTECHNOLOGY; CELL SURVIVAL; CHO CELLS; CRICETINAE; CRICETULUS; CULTURE MEDIA; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; PROTEINS; PROTEOMICS;

EID: 74849111728     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.22532     Document Type: Article

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