The dynamics of the CHO host cell protein profile during clarification and protein A capture in a platform antibody purification process

Biotechnology and Bioengineering

Volumn 110, Issue 1, 2013, Pages 240-251

  Hogwood, Catherine E M ^a   Tait, Andrew S ^b   Koloteva Levine, Nadejda ^a   Bracewell, Daniel G ^b   Smales, C Mark ^a  

^a UNIVERSITY OF KENT   (United Kingdom)

^b UNIVERSITY COLLEGE LONDON   (United Kingdom)

Author keywords

2D PAGE; Chinese hamster ovary cells; Downstream processing; Host cell protein; Monoclonal antibody; Protein A chromatography

Indexed keywords

CELL CULTURE; CELLS; CENTRIFUGATION; CHROMATOGRAPHY; CLARIFIERS; DYNAMICS; IMPURITIES; MONOCLONAL ANTIBODIES; RECOMBINANT PROTEINS;

2D-PAGE; CHINESE HAMSTER OVARY CELLS; DOWNSTREAM-PROCESSING; HOST CELL PROTEIN; PROTEIN A CHROMATOGRAPHY;

CELL ENGINEERING;

CELL PROTEIN; ENOLASE; GLUCOSE REGULATED PROTEIN 78; HEAT SHOCK PROTEIN; HOST CELL PROTEIN; IMMUNOGLOBULIN HEAVY CHAIN; IMMUNOGLOBULIN LIGHT CHAIN; MONOCLONAL ANTIBODY; PROTEIN A; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; CELL ENGINEERING; CELL FATE; CELL LINE; CENTRIFUGATION; CHO CELL; CHROMATOGRAPHY; CONTROLLED STUDY; DOWNSTREAM PROCESSING; ELUTION; FILTRATION; HEAVY CHAIN; HOST CELL; INTRACELLULAR SPACE; LIGHT CHAIN; MOLECULAR DYNAMICS; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN A CAPTURE CHROMATOGRAPHY; PROTEIN ANALYSIS; PROTEIN PURIFICATION; PROTEOMICS; SUPERNATANT; TWO DIMENSIONAL GEL ELECTROPHORESIS;

CRICETULUS GRISEUS;

EID: 84869876139     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.24607     Document Type: Article

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