Transgene copy number distribution profiles in recombinant CHO cell lines revealed by single cell analyses

Biotechnology and Bioengineering

Volumn 109, Issue 7, 2012, Pages 1713-1722

  He, Luhong ^a   Winterrowd, Christal ^a   Kadura, Ibrahim ^a   Frye, Christopher ^a  

^a ELI LILLY AND COMPANY   (United States)

Author keywords

Cell line suitability; CHO cell line; Population drift; QPCR; Recombinant protein production; Single cell; Transgene copy number distribution

Indexed keywords

CELL LINES; CHO CELL; POPULATION DRIFT; QPCR; RECOMBINANT PROTEIN PRODUCTIONS; SINGLE CELLS; TRANSGENE COPY NUMBERS;

CELL CULTURE; CLONING; GENES; POLYMERASE CHAIN REACTION; POPULATION DISTRIBUTION; PRODUCTIVITY; RECOMBINANT PROTEINS;

CLONE CELLS;

ANIMAL CELL; ARTICLE; CELL HETEROGENEITY; CHO CELL; CONTROLLED STUDY; COPY NUMBER VARIATION; NONHUMAN; POLYMERASE CHAIN REACTION; QUANTITATIVE POLYMERASE CHAIN REACTION; TRANSGENE; TRANSGENE COPY NUMBER;

ANIMALS; CHO CELLS; CRICETINAE; GENE DOSAGE; POLYMERASE CHAIN REACTION; SINGLE-CELL ANALYSIS; TRANSGENES;

CRICETULUS GRISEUS;

EID: 84861199646     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.24428     Document Type: Article

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