Recovery of Chinese hamster ovary host cell proteins for proteomic analysis

Biotechnology Journal

Volumn 9, Issue 1, 2014, Pages 87-99

  Valente, Kristin N ^a   Schaefer, Amy K ^a   Kempton, Hannah R ^a   Lenhoff, Abraham M ^a   Lee, Kelvin H ^a  

^a UNIVERSITY OF DELAWARE   (United States)

Author keywords

Chinese hamster ovary cells; Design of experiments optimization; Extracellular host cell proteins; Proteomics; Sample preparation

Indexed keywords

CHINESE HAMSTER OVARY; CHINESE HAMSTER OVARY CELLS; HOST CELL PROTEIN; MANUFACTURING OPERATIONS; PROTEIN CONCENTRATIONS; PROTEOMICS; SAMPLE PREPARATION; TWO DIMENSIONAL ELECTROPHORESIS;

CELLS; CYTOLOGY; DESIGN OF EXPERIMENTS; ELECTROPHORESIS; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; OPTIMIZATION; PROTEINS; RECOVERY;

MOLECULAR BIOLOGY;

CELL PROTEIN; HOST CELL PROTEIN; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; CHO CELL; CONCENTRATION (PARAMETERS); DIELECTRIC CONSTANT; FEMALE; HOST CELL; HYDROPHOBICITY; INCUBATION TIME; LIQUID CHROMATOGRAPHY; MOLECULAR WEIGHT; NONHUMAN; PRECIPITATION; PRIORITY JOURNAL; PROCESS OPTIMIZATION; PROTEOMICS; TANDEM MASS SPECTROMETRY; TWO DIMENSIONAL ELECTROPHORESIS;

CRICETULUS GRISEUS;

CHINESE HAMSTER OVARY CELLS; DESIGN OF EXPERIMENTS OPTIMIZATION; EXTRACELLULAR HOST CELL PROTEINS; PROTEOMICS; SAMPLE PREPARATION;

ANIMALS; CHO CELLS; CRICETINAE; CRICETULUS; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; HIGH-THROUGHPUT SCREENING ASSAYS; PROTEOME; PROTEOMICS; RESEARCH DESIGN; SOLVENTS;

EID: 84891936728     PISSN: 18606768     EISSN: 18607314     Source Type: Journal    
DOI: 10.1002/biot.201300190     Document Type: Article

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