Speeding up directed evolution: Combining the advantages of solid-phase combinatorial gene synthesis with statistically guided reduction of screening effort

ACS Synthetic Biology

Volumn 4, Issue 3, 2015, Pages 317-331

  Hoebenreich, Sabrina ^a,b   Zilly, Felipe E ^a   Acevedo Rocha, Carlos G ^a,b   Zilly, Matías ^c   Reetz, Manfred T ^a,b  

^a MAX PLANCK INSTITUT FÜR KOHLENFORSCHUNG   (Germany)

^b PHILIPPS UNIVERSITY MARBURG   (Germany)

^c UNIVERSITY OF DUISBURG ESSEN   (Germany)

Author keywords

biocatalysis; cytochrome P450; genome engineering; metabolic engineering; protein engineering; screening effort

Indexed keywords

ALGORITHM; AMINO ACID SEQUENCE; ARTICLE; COMBINATORIAL LIBRARY; GENE SYNTHESIS; MOLECULAR LIBRARY; MUTAGENESIS; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SCREENING; SOLID PHASE SYNTHESIS; STATISTICS; BIOLOGICAL MODEL; DIRECTED MOLECULAR EVOLUTION; ESCHERICHIA COLI; GENE LIBRARY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HYDROXYLATION; MOLECULAR BIOLOGY; MUTATION; PROCEDURES;

TESTOSTERONE;

CHROMATOGRAPHY, HIGH PRESSURE LIQUID; DIRECTED MOLECULAR EVOLUTION; ESCHERICHIA COLI; GENE LIBRARY; HYDROXYLATION; MODELS, GENETIC; MOLECULAR BIOLOGY; MUTATION; POLYMERASE CHAIN REACTION; TESTOSTERONE;

EID: 84925611687     PISSN: None     EISSN: 21615063     Source Type: Journal    
DOI: 10.1021/sb5002399     Document Type: Article

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