Humanization of antibodies using heavy chain complementarity-determining region 3 grafting coupled with in vitro somatic hypermutation

Journal of Biological Chemistry

Volumn 288, Issue 11, 2013, Pages 7688-7696

  Bowers, Peter M ^a   Neben, Tamlyn Y ^a   Tomlinson, Geoffery L ^a   Dalton, Jennifer L ^a   Altobell, Larry ^a   Zhang, Xue ^a   MacOmber, John L ^a   Wu, Betty F ^a   Toobian, Rachelle M ^a   McConnell, Audrey D ^a   Verdino, Petra ^a   Chau, Betty ^a   Horlick, Robert A ^a   King, David J ^a  

^a AnaptysBio Inc   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AFFINITY MATURATION; COMPLEMENTARITY-DETERMINING REGION 3; HEK293 CELLS; HIGH AFFINITY; HUMANIZED ANTIBODY; LIGHT CHAIN; POTENT INHIBITION; SOMATIC HYPERMUTATION;

MONOCLONAL ANTIBODIES;

GRAFTING (CHEMICAL);

NERVE GROWTH FACTOR BETA SUBUNIT;

AMINO ACID SEQUENCE; ANTIBODY AFFINITY; ANTIBODY COMBINING SITE; ANTIBODY ENGINEERING; ANTIBODY STRUCTURE; ARTICLE; CELL SURFACE; COMPLEMENTARITY DETERMINING REGION; CONTROLLED STUDY; HEAVY CHAIN; IN VITRO STUDY; IN VIVO STUDY; LIGHT CHAIN; PRIORITY JOURNAL; PROCESS DEVELOPMENT; PROTEIN FUNCTION; SOMATIC HYPERMUTATION;

ANIMALS; ANTIBODIES; ANTIBODIES, MONOCLONAL; ANTIGENS; BASE SEQUENCE; BINDING, COMPETITIVE; CELL SEPARATION; CODON; COMPLEMENTARITY DETERMINING REGIONS; CYTOKINES; FLOW CYTOMETRY; HEK293 CELLS; HUMANS; MICE; MODELS, GENETIC; MOLECULAR SEQUENCE DATA; MUTATION; PROTEIN ENGINEERING; SIGNAL TRANSDUCTION;

EID: 84875169292     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M112.445502     Document Type: Article

References (33)

1. Reichert, J. M., (2011) Antibody-based therapeutics to watch in 2011. mAbs 3, 76-99
2. Morrison, S. L., Johnson, M. J., Herzenberg, L. A., and Oi, V. T. (1984) Chimeric antibody molecules. Mouse antigen-binding domains with human constant region domains. Proc. Natl. Acad. Sci. U.S.A. 81, 6851-6855
3. Jones, P. T., Dear, P. H., Foote, J., Neuberger, M. S., and Winter, G. (1986) Replacing the complementarity-determining regions in a human antibody with those from a mouse. Nature 321, 522-525
4. Verhoeyen, M., Milstein, C., and Winter, G. (1988) Reshaping human antibodies. Grafting an antilysozyme activity. Science 239, 1534-1536
5. Queen, C., Schneider, W. P., Selick, H. E., Payne, P. W., Landolfi, N. F., Duncan, J. F., Avdalovic, N. M., Levitt, M., Junghans, R. P., and Waldmann, T. A. (1989) A humanized antibody that binds to the interleukin 2 receptor. Proc. Natl. Acad. Sci. U.S.A. 86, 10029-10033
6. Tan, P., Mitchell, D. A., Buss, T. N., Holmes, M. A., Anasetti, C., and Foote, J. (2002) "Superhumanized" antibodies. Reduction of immunogenic potetial by complementarity-determining region grafting with human germline sequence. Application to an anti-CD28. J. Immunol. 169, 1119-1125
7. Lazar, G. A., Desjarlais, J. R., Jacinto, J., Karki, S., and Hammond, P. W. (2007) A molecular immunology approach to antibody humanization and functional optimization. Mol. Immunol. 44, 1986-1998
8. Baca, M., Presta, L. G., O'Connor, S. J., and Wells, J. A. (1997) Antibody humanization using phage display. J. Biol. Chem. 272, 10678-10684
9. Gram, H., Marconi, L.-A., Barbas, C. F., 3rd, Collet, T. A., Lerner, R. A., and Kang, A. S. (1992) In vitro selection and affinity maturation of antibodies from a naïve combinatorial immunoglobulin library. Proc. Natl. Acad. Sci. U.S.A. 89, 3576-3580
10. Hawkins, R. E., Russell, S. J., and Winter, G. (1992) Selection of phage antibodies by binding affinity. Mimicking affinity maturation. J. Mol. Biol. 226, 889-896
11. Yang, W. P., Green, K., Pinz-Sweeney S., Briones, A. T., Burton, D. R., and Barbas, C. F., 3rd (1995) CDR walking mutagenesis for the affinity maturation of a potent human anti-HIV-1 antibody into the picomolar range. J. Mol. Biol. 254, 392-403
12. Ho, M., Kreitman, R. J., Onda, M., and Pastan, I. (2005) In vitro antibody evolution targeting germline hot spots to increase activity of an anti-CD22 immunotoxin. J. Biol. Chem. 280, 607-617
13. Ho, M., Nagata, S., and Pastan, I. (2006) Isolation of anti-CD22 Fv with high affinity by Fv display on human cells. Proc. Natl. Acad. Sci. U.S.A. 103, 9637-9642
14. Bowers, P. M., Horlick, R. A., Neben, T. Y., Toobian, R. M., Tomlinson, G. L., Dalton, J. L., Jones, H. A., Chen, A., Altobell, L., 3rd, Zhang, X., Macomber, J. L., Krapf, I. P., Wu, B. F., McConnell, A., Chau, B., Holland, T., Berkebile, A. D., Neben, S. S., Boyle, W. J., and King, D. J. (2011) Coupling mammalian cell surface display with somatic hypermutation for the discovery and maturation of human antibodies. Proc. Natl. Acad. Sci. U.S.A. 108, 20455-20460
15. Rogozin, I. B., and Diaz, M. (2004) Cutting edge. DGYW/WRCH is a better predictor of mutability at G:C bases in Ig hypermuation than the widely accepted RGYW/WRCY motif and probably reflects a two-step activiation-induced cytidine deaminase-triggered process. J. Immunol. 172, 3382-3384
16. Martin, A., and Scharff, M. (2002) Somatic hypermutation of the AID transgene in B and non-B cells. Proc. Natl. Acad. Sci. U.S.A. 99, 12304-12308
17. Cumbers, S. J., Williams, G. T., Davies, S. L., Grenfell, R. L., Takeda, S., Batista, F. D., Sale, J. E., and Neuberger, M. S. (2002) Generation and iterative affinity maturation of antibodies in vitro using hypermutating B-cell lines. Nat. Biotechnol. 20, 1129-1134
18. Zheng, N. Y., Wilson, K., Jared, M., and Wilson, P. C. (2005) Intricate targeting of immunoglobulin somatic hypermutation maximizes the efficiency of affinity maturation. J. Exp. Med. 201, 1467-1478
19. Van den Brulle, J., Fischer, M., Langmann, T., Horn, G., Waldmann, T., Arnold, S., Fuhrmann, M., Schatz, O., O'Connell, T., O'Connell, D., Auckenthaler, A., and Schwer, H. (2008) A novel solid phase technology for high-throughput gene synthesis. BioTechniques 45, 340-343
20. Wu, T. T., and Kabat, E. A. (1970) An analysis of the sequences of the variable regions of Bence Jones proteins and myeloma light chains and their implications for antibody complementarity. J. Exp. Med. 132, 211-250
21. Lefranc, M.-P. (2004) IMGT-ONTOLOGY and IMGT databases, tools and web resources for immunogenetics and immunoinformatics. Mol. Immunol. 40, 647-660
22. Rogers, J., Choi, E., Souza, L., Carter, C., Word, C., Kuehl, M., Eisenberg, D., and Wall, R. (1981) Gene segments encoding transmembrane carboxyl termini of immunoglobulin gamma chains. Cell 26, 19-27
23. Greene, L. A., and Tischler, A. S. (1976) Establishment of a noradrenergic clonal line of rat adrenalpheochromocytoma cells which respond to nerve growth factor. Proc. Natl. Acad. Sci. U.S.A. 73, 2424-2428
24. Cattaneo, A., Rapposelli, B., and Calissano, P. (1988) Three distinct types of monoclonal antibodies after long-term immunization of rats with mouse nerve growth factor. J. Neurochem. 50, 1003-1010
25. Chao, G., Lau, W. L., Hackel, B. J., Sazinsky, S. L., Lippow, S. M., and Wittrup, K. D. (2006) Isolating and engineering human antibodies using yeast surface display. Nat. Protoc. 1, 755-768
26. Azoitei, M. L., Correia, B. E., Ban, Y. E., Carrico, C., Kalyuzhniy, O., Chen, L., Schroeter, A., Huang, P. S., McLellan, J. S., Kwong, P. D., Baker, D., Strong, R. K., and Schief, W. R. (2011) Computation-guided backbone grafting of a discontinuous motif onto a protein scaffold. Science 334, 373-376
27. Azoitei, M. L., Ban, Y. E., Julien, J. P., Bryson, S., Schroeter, A., Kalyuzhniy, O., Porter, J. R., Adachi, Y., Baker, D., Pai, E. F., and Schief, W. R. (2012) Computational design of high-affinity epitope scaffolds by backbone grafting of a linear epitope. J. Mol. Biol. 415, 175-192
28. Fleishman, S. J., Whitehead, T. A., Ekiert, D. C., Dreyfus, C., Corn, J. E., Strauch, E. M., Wilson, I. A., and Baker, D. (2011) Computational design of proteins targeting the conserved stem region of influenza hemagglutinin. Science 332, 816-821
29. Cattaneo, A., Covaceuszach, S., and Lamba. D. (2005) Method for the humanization of antibodies and humanized antibodies thereby obtained. U. S. Patent WO/2005/061540
30. McConnell, A. D., Do, M., Neben, T. Y., Spasojevic, V., MacLaren, J., Chen, A. P., Altobell, L., 3rd, Macomber, J. L., Berkebile, A. D., Horlick, R. A., Bowers, P. M., and King, D. J. (2012) Generation of high affinity humanized antibodies without making hybridomas. Immunization paired with mammalian cell display and in vitro somatic hypermutation. PLoS One 7, e49458
31. Hoet, R. M., Cohen, E. H., Kent, R. B., Rookey, K., Schoonbroodt, S., Hogan, S., Rem, L., Frans, N., Daukandt, M., Pieters, H., van Hegelsom, R., Neer, N. C., Nastri, H. G., Rondon, I. J., Leeds, J. A., Hufton, S. E., Huang, L., Kashin, I., Devlin, M., Kuang, G., Steukers, M., Viswanathan, M., Nixon, A. E., Sexton, D. J., Hoogenboom, H. R., Ladner, R. C., (2005) Generation of high-affinity human antibodies by combining donor-derived and synthetic complementarity-determining-region diversity. Nat. Biotechnol. 23, 344-348
32. Chowdhury, P. S., and Pastan, I. (1999) Improving antibody affinity by mimicking somatic hypermutation in vitro. Nat. Biotechnol. 17, 568-572
33. Covaceuszach, S., Cassetta, A., Konarev, P. V., Gonfloni, S., Rudolph, R., Svergun, D. I., Lamba, D., and Cattaneo, A. (2008) Dissecting hßNGF interactions with TrkA and p75 receptors by structural and functional studies of an anti-NGF neutralizing antibody. J. Mol. Biol. 381, 881-896