ProxiMAX randomization: A new technology for non-degenerate saturation mutagenesis of contiguous codons

Biochemical Society Transactions

Volumn 41, Issue 5, 2013, Pages 1189-1194

  Ashraf, Mohammed ^a   Frigotto, Laura ^b   Smith, Matthew E ^b   Patel, Seema ^b   Hughes, Marcus D ^a   Poole, Andrew J ^a   Hebaishi, Husam R M ^a   Ullman, Christopher G ^b   Hine, Anna V ^a  

^a ASTON UNIVERSITY   (United Kingdom)

^b The Mansion   (United Kingdom)

Author keywords

Antibody engineering; Gene library; MAX randomization; Overlap PCR; Protein engineering; Saturation mutagenesis

Indexed keywords

AMINO ACID; CONTIG; DNA; DOUBLE STRANDED DNA; OLIGONUCLEOTIDE; PHOSPHORAMIDOUS ACID; POLYDEOXYRIBONUCLEOTIDE SYNTHASE; REAGENT; RESTRICTION ENDONUCLEASE; TRINUCLEOTIDE; ZINC FINGER PROTEIN;

ACCURACY; ALPHA HELIX; AMINO ACID ANALYSIS; AMINO ACID SEQUENCE; ANTIBODY ENGINEERING; ARTICLE; CHEMICAL ANALYZER; CHEMISTRY; CODON; CYCLING; DIGESTION; DNA SEQUENCE; ENZYME ENGINEERING; GENE AMPLIFICATION; GENE CASSETTE; MOLECULAR BIOLOGY; MUTAGENESIS; NONDEGENERATE SATURATION MUTAGENESIS; PRIORITY JOURNAL; PROTEIN ENGINEERING; PROTEIN ISOLATION; PROTEIN LOCALIZATION; RANDOMIZATION; TECHNOLOGY; ZINC FINGER MOTIF;

ANTIBODIES; CODON; GENE LIBRARY; GENETIC CODE; HUMANS; MUTAGENESIS; PROTEIN ENGINEERING; PROTEINS;

EID: 84884666223     PISSN: 03005127     EISSN: 14708752     Source Type: Journal    
DOI: 10.1042/BST20130123     Document Type: Article

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