Laccase engineering: From rational design to directed evolution

Biotechnology Advances

Volumn 33, Issue 1, 2015, Pages 25-40

  Mate, Diana M ^a   Alcalde, Miguel ^b  

^a DWI LEIBNIZ INSTITUTE FOR INTERACTIVE MATERIALS   (Germany)

^b INSTITUTO DE CATÁLISIS Y PETROLEOQUÍMICA   (Spain)

Author keywords

Bacterial laccase; Directed evolution; DNA recombination; Functional expression; Fungal laccase; Laccase chimeras; Rational design; Redox potential; Saturation mutagenesis

Indexed keywords

BIOFUELS; BIOLOGICAL FUEL CELLS; BIOREMEDIATION; CATALYST ACTIVITY; IONIC LIQUIDS; MOLECULAR OXYGEN; REDOX REACTIONS; SUBSTRATES;

BACTERIAL LACCASE; DIRECTED EVOLUTION; DNA RECOMBINATION; FUNCTIONAL EXPRESSION; FUNGAL LACCASES; LACCASES; RATIONAL DESIGN; REDOX POTENTIALS; SATURATION MUTAGENESIS;

ENZYMES;

BACTERIUM; BIOENGINEERING; ENZYME ACTIVITY; EVOLUTIONARY THEORY; MUTAGENICITY; RECOMBINATION; REDOX POTENTIAL; SUBSTRATE;

BACTERIA (MICROORGANISMS);

BACTERIAL PROTEIN; FUNGAL PROTEIN; LACCASE;

CHEMISTRY; DIRECTED MOLECULAR EVOLUTION; ENZYME SPECIFICITY; GENETICS; PROTEIN CONFORMATION; PROTEIN ENGINEERING;

BACTERIAL PROTEINS; DIRECTED MOLECULAR EVOLUTION; FUNGAL PROTEINS; LACCASE; PROTEIN CONFORMATION; PROTEIN ENGINEERING; SUBSTRATE SPECIFICITY;

EID: 84922865301     PISSN: 07349750     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.biotechadv.2014.12.007     Document Type: Review

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