Molecular cloning and heterologous expression of a laccase gene from Pleurotus eryngii in free and immobilized Saccharomyces cerevisiae cells

Applied Microbiology and Biotechnology

Volumn 79, Issue 5, 2008, Pages 731-741

  Bleve, Gianluca ^a   Lezzi, Chiara ^b   Mita, Giovanni ^a   Rampino, Patrizia ^b   Perrotta, Carla ^b   Villanova, Luciano ^c   Grieco, Francesco ^a  

^a INSTITUTE OF SCIENCES OF FOOD PRODUCTION   (Italy)

^b UNIVERSITY OF SALENTO   (Italy)

^c Lachifarma   (Italy)

Author keywords

Ca alginate immobilization; Laccase; Pleurotus eryngii; Recombinant protein in yeast

Indexed keywords

CELLS; CLONING; GENE EXPRESSION; PROTEINS; YEAST;

CA-ALGINATE IMMOBILIZATION; LACCASE; PLEUROTUS ERYNGII; RECOMBINANT PROTEIN IN YEAST;

MOLECULAR BIOLOGY;

AMINO ACID; CALCIUM ALGINATE; CALCIUM CHLORIDE; COMPLEMENTARY DNA; COPPER; ISOENZYME; LACCASE; RECOMBINANT ENZYME; RECOMBINANT PROTEIN;

AMINO ACID; CLONE; DNA; ENZYME; FUNGUS; MOLECULAR ANALYSIS; MUSHROOM; POLYMERASE CHAIN REACTION;

AMINO ACID SEQUENCE; ARTICLE; BACTERIAL GENE; BATCH FERMENTATION; ENZYME IMMOBILIZATION; ENZYME STABILITY; ENZYME SYNTHESIS; FUNGAL STRAIN; FUNGUS CULTURE; GENE EXPRESSION; GENE ISOLATION; HETEROLOGOUS EXPRESSION; IMMOBILIZED CELL; MICROBIAL BIOMASS; MOLECULAR CLONING; MOLECULAR WEIGHT; MYCELIUM; NONHUMAN; NUCLEOTIDE SEQUENCE; PLEUROTUS; PLEUROTUS ERYNGII; PREDICTION; PROTEIN DEGRADATION; PROTEIN STABILITY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SACCHAROMYCES CEREVISIAE;

BIOMASS; CELLS, IMMOBILIZED; CLONING, MOLECULAR; ENZYME STABILITY; FUNGAL PROTEINS; GENE EXPRESSION; LACCASE; PLEUROTUS; RECOMBINANT PROTEINS; SACCHAROMYCES CEREVISIAE;

BASIDIOMYCOTA; PLEUROTUS ERYNGII; SACCHAROMYCES CEREVISIAE;

EID: 46549089413     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-008-1479-1     Document Type: Article

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