Enhancement of a bacterial laccase thermostability through directed mutagenesis of a surface loop

Enzyme and Microbial Technology

Volumn 49, Issue 5, 2011, Pages 446-452

  Mollania, Nasrin ^a   Khajeh, Khosro ^a   Ranjbar, Bijan ^a   Hosseinkhani, Saman ^a  

^a TARBIAT MODARES UNIVERSITY   (Iran)

Author keywords

Laccase; Site directed mutagenesis; Thermal activation; Thermostability

Indexed keywords

BENZENEDIOL; BIOTECHNOLOGICAL PROCESS; CATALYTIC SITES; CHEMICAL DENATURANTS; DRUG ANALYSIS; HIGHER T; LACCASES; NATIVE ENZYMES; OXIDOREDUCTASES; POINT MUTATIONS; POLYPHENOLS; SITE DIRECTED MUTAGENESIS; SURFACE LOOPS; THERMAL ACTIVATION; THERMOSTABILITY;

BACTERIOLOGY; BIOREMEDIATION; BIOTECHNOLOGY; CHEMICAL STABILITY; DICHROISM; ENZYMES; PHENOLS; THERMODYNAMIC STABILITY; UREA;

MUTAGENESIS;

BACTERIAL ENZYME; LACCASE; RECOMBINANT ENZYME; UREA;

AEROBIC METABOLISM; ARTICLE; BACILLUS; CATALYSIS; CIRCULAR DICHROISM; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME DENATURATION; ENZYME PURIFICATION; ENZYME STABILITY; ENZYME SYNTHESIS; FLUORESCENCE; HEAT TOLERANCE; MUTATIONAL ANALYSIS; NONHUMAN; POINT MUTATION; PROTEIN STRUCTURE; SITE DIRECTED MUTAGENESIS; WILD TYPE;

AMINO ACID SUBSTITUTION; BACILLUS; BACTERIAL PROTEINS; CIRCULAR DICHROISM; ENZYME ACTIVATION; ENZYME STABILITY; KINETICS; LACCASE; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; PROTEIN CONFORMATION; PROTEIN DENATURATION; RECOMBINANT PROTEINS; SPECTROMETRY, FLUORESCENCE; THERMODYNAMICS; UREA;

BACILLUS (BACTERIUM); BACTERIA (MICROORGANISMS);

EID: 80054702661     PISSN: 01410229     EISSN: 18790909     Source Type: Journal    
DOI: 10.1016/j.enzmictec.2011.08.001     Document Type: Article

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