Molecular evolution of Fome lignosus laccase by ethyl methane sulfonate-based random mutagenesis in vitro

Biomolecular Engineering

Volumn 24, Issue 6, 2007, Pages 619-624

  Hu, Mei Rong ^a,b   Chao, Ya Peng ^a   Zhang, Guo Qing ^a   Yang, Xiu Qing ^c   Xue, Zhi Quan ^a,b   Qian, Shi Jun ^a  

^a INSTITUTE OF MICROBIOLOGY   (China)

^b UNIVERSITY OF CHINESE ACADEMY OF SCIENCES   (China)

^c SHANXI UNIVERSITY   (China)

Author keywords

Ethyl methane sulfonate; Laccase; Pichia pastoris

Indexed keywords

ETHYL METHANE SULFONATE; LACCASE; PICHIA PASTORIS;

AMINO ACIDS; DNA SEQUENCES; GENE EXPRESSION; MUTAGENESIS; RANDOM PROCESSES; THERMAL EFFECTS;

MOLECULAR STRUCTURE;

2,2' AZINOBIS 2 ETHYLBENZOTHIAZOLINE 6 SULFONIC ACID; COPPER; GUAIACOL; LACCASE; MESYLIC ACID ETHYL ESTER; SULFONIC ACID DERIVATIVE; UNCLASSIFIED DRUG;

ARTICLE; ENZYME ACTIVITY; ENZYME PURIFICATION; IN VITRO STUDY; INCUBATION TEMPERATURE; MOLECULAR EVOLUTION; MUTAGENESIS; NONHUMAN; PH; PICHIA PASTORIS; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; SEQUENCE ANALYSIS; WILD TYPE; YEAST;

BENZOTHIAZOLES; CLONING, MOLECULAR; DIRECTED MOLECULAR EVOLUTION; ETHYL METHANESULFONATE; LACCASE; MODELS, MOLECULAR; MUTAGENESIS; SUBSTRATE SPECIFICITY; SULFONIC ACIDS; YEASTS;

LIGNOSUS; PICHIA PASTORIS;

EID: 36249008058     PISSN: 13890344     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bioeng.2007.08.020     Document Type: Article

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