Heterologous laccase production and its role in industrial applications

Bioengineered Bugs

Volumn 1, Issue 4, 2010, Pages 252-262

  Alessandra, Piscitelli ^a   Cinzia, Pezzella ^a   Paola, Giardina ^a   Vincenza, Faraco ^a   Sannia, Giovanni ^a  

^a UNIVERSITY OF NAPLES FEDERICO II   (Italy)

Author keywords

Directed evolution; Filamentous fungi; Heterologous expression; Industrial application; Laccase; Yeasts

Indexed keywords

BACTERIAL ENZYME; COMPLEMENTARY DNA; COPPER PROTEIN; FUNGAL ENZYME; LACCASE; RECOMBINANT ENZYME; RECOMBINANT PROTEIN;

BACILLUS SUBTILIS; BIOCATALYSIS; BIOPULPING; BIOSENSOR; CARBOXY TERMINAL SEQUENCE; DECOLORIZATION; ENZYME SYNTHESIS; ESCHERICHIA COLI; FERMENTATION OPTIMIZATION; FOOD INDUSTRY; HETEROLOGOUS EXPRESSION; INDUSTRIAL PRODUCTION; MUTAGENESIS; OXIDATION; PHYTOREMEDIATION; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN EXPRESSION; PROTEIN SYNTHESIS; PURIFICATION; REVIEW; STRUCTURE ACTIVITY RELATION; YEAST;

BIOTECHNOLOGY; LACCASE;

FUNGI;

EID: 79960251980     PISSN: 19491018     EISSN: 19491026     Source Type: Journal    
DOI: None     Document Type: Review

References (168)

1. Yoshida H. Chemistry of Lacquer (Urishi) part 1. J Chem Soc 1883; 43:472-86.
2. Hoopes JT, Dean JF. Ferroxidase activity in a laccase-like multicopper oxidase from Liriodendron tulipifera. Plant Physiol Biochem 2004; 42:27-33.
3. Laborde J. Sur la casse des vins. C R Hebd Seances Acad Sci 1896; 123:1074-5.
4. Giardina P, Faraco V, Pezzella C, Piscitelli A, Vanhulle S, Sannia G. Laccases: a never-ending story. Cell Mol Life Sci 2010; 67:369-85.
5. Dittmer NT, Suderman RJ, Jiang H, Zhu YC, Gorman MJ, Kramer KJ, et al. Characterization of cDNAs encoding putative laccase-like multicopper oxidases and developmental expression in the tobacco hornworm, Manduca sexta, and the malaria mosquito, Anopheles gambiae. Insect Biochem Mol Biol 2004; 34:29-41.
6. Sharma P, Goel R, Capalash N. Bacterial laccases. World J Microbiol Biotechnol 2007; 23:823-32.
7. Molina-Guijarro JM, Pérez J, Muñoz-Dorado J, Guillén F, Moya R, Hernández M, Arias ME. Detoxification of azo dyes by a novel pH-versatile, salt-resistant laccase from Streptomyces ipomoea. Int Microbiol 2009; 12:13-21.
8. Martins LO, Soares CM, Pereira MM, Teixeira M, Costa T, Jones GH, et al. Molecular and biochemical characterization of a highly stable bacterial laccase that occurs as a structural component of the Bacillus subtilis endospore coat. J Biol Chem 2002; 277:18849-59.
9. Messerschmidt A. Multi-Copper Oxidases. World Scientific, Singapore 1997.
10. Kunamneni A, Camarero S, García-Burgos C, Plou FJ, Ballesteros A, Alcalde M. Engineering and applications of fungal laccases for organic synthesis. Microb cell fact 2008; 7:32-49.
11. Bourbonnais R, Leech D, Paice MG. Electrochemical analysis of the interactions of laccase mediators with lignin model compounds. Biochim Biophys Acta 1998; 1379:381-90.
12. Vinod S. Enzymatic decolourisation of denims: a novel approach. Colourage 2001; 48:25-6.
13. Minussi R, Pastore GM, Duran N. Potential applications of laccase in the food industry. Trends Food Sci Technol 2002; 13:205-16.
14. Widsten P, Kandelbauer A. Laccase applications in the forest products industry: a review. Enzyme Microb Technol 2008; 42:293-307.
15. Nicotra S, Cramarossa MR, Mucci A, Pagnoni UM, Riva S, Forti L. Biotransformation of resveratrol: synthesis of trans-dehydrodimers catalyzed by laccases from Myceliophtora thermophyla and from Trametes pubescens. Tetrahedron 2004; 60:595-600.
16. Bruyneel F, Enaud E, Billottet L, Vanhulle S, Marchand- Brynaert J. Regioselective synthesis of 3-hydroxyorthanilic acid and its biotransformation with laccase into a novel phenoxazinone dye. Eur J Org Chem 2008; 1:72-9.
17. Gübitz GM, Cavaco Paulo A. New substrates for reliable enzymes: enzymatic modification of polymers. Curr Opin Biotechnol 2003; 14:577-82.
18. Kulys J, Vidziunaite R. Amperometric biosensors based on recombinant laccases for phenols determination. Biosens Bioelectron 2003; 18:319-25.
19. Amir L, Tam TK, Pita M, Meijler MM, Alfonta L, Katz E. Biofuel cell controlled by enzyme logic systems. J Am Chem Soc 2009; 131:826-32.
20. Kunamneni A, Plou FJ, Ballesteros A, Alcalde M. Laccases and their applications: a patent review. Recent Pat Biotechnol 2008; 2:10-24.
21. Faraco V, Pezzella C, Miele A, Giardina P, Sannia G. Bio-remediation of colored industrial wastewaters by the white-rot fungi Phanerochaete chrysosporium and Pleurotus ostreatus and their enzymes. Biodegradation 2009; 20:209-20.
22. Olivieri G, Marzocchella A, Salatino P, Giardina P, Cennamo G, Sannia G. Olive mill wastewater remediation by means of Pleurotus ostreatus. Biochem Eng J 2006; 31:180-7.
23. Ferrer-Miralles N, Domingo-Espín J, Corchero JL, Vázquez E, Villaverde A. Microbial factories for recombinant pharmaceuticals. Microb Cell Fact 2009; 24:8-17.
24. Demain AL, Vaishnav P. Production of recombinant proteins by microbes and higher organisms. Biotechnol Adv 2009; 27:297-306.
25. Schilling B, Linden RM, Kupper U, Lerch K. Expression of Neurospora crassa laccase under the control of the copper-inducible metallothionein-promoter. Curr Genet 1992; 22:197-203.
26. Alves AM, Record E, Lomascolo A, Scholtmeijer K, Asther M, Wessels JG, et al. Highly efficient production of laccase by the basidiomycete Pycnoporus cinnabarinus. Appl Environ Microbiol 2004; 70:6379-84.
27. Kajita S, Sugawara S, Miyazaki Y, Nakamura M, Katayama Y, Shishido K, et al. Overproduction of recombinant laccase using a homologous expression system in Coriolus versicolor. Appl Microbiol Biotechnol 2004; 66:194-9.
28. Waterman SR, Hacham M, Panepinto J, Hu G, Shin S, Williamson PR. Cell wall targeting of laccase of Cryptococcus neoformans during infection of mice. Infect Immun 2007; 75:714-22.
29. Mander GJ, Wang H, Bodie E, Wagner J, Vienken K, Vinuesa C, et al. Use of laccase as a novel, versatile reporter system in filamentous fungi. Appl Environ Microbiol 2006; 72:5020-6.
30. Kataoka K, Komori H, Ueki Y, Konno Y, Kamitaka Y, Kurose S, et al. Structure and function of the engineered multicopper oxidase CueO from Escherichia coli-deletion of the methionine-rich helical region covering the substrate-binding site. J Mol Biol 2007; 373:141-52.
31. Bento I, Martins LO, Gato Lopes G, Arménia Carrondo M, Lindley PF. Dioxygen reduction by multi-copper oxidases; a structural perspective. Dalton Trans 2005; 21:3507-13.
32. Brissos V, Pereira L, Munteanu FD, Cavaco-Paulo A, Martins LO. Expression system of CotA-laccase for directed evolution and high-throughput screenings for the oxidation of high-redox potential dyes. Biotechnol J 2009; 4:558-63.
33. Suzuki T, Endo K, Ito M, Tsujibo H, Miyamoto K, Inamori Y. A thermostable laccase from Streptomyces lavendulae REN-7: purification, characterization, nucleotide sequence and expression. Biosci Biotechnol Biochem 2003; 67:2167-75.
34. Dubé E, Shareck F, Hurtubise Y, Daneault C, Beauregard M. Homologous cloning, expression, and characterisation of a laccase from Streptomyces coelicolor and enzymatic decolourisation of an indigo dye. Appl Microbiol Biotechnol 2008; 79:579-603.
35. Salony, Garg N, Baranwal R, Chhabra M, Mishra S, Chaudhuri TK, et al. Laccase of Cyathus bulleri: structural, catalytic characterization and expression in Escherichia coli. Biochim Biophys Acta 2008; 1784:259-68.
36. Sanchez-Amat A, Lucas-Elío P, Fernández E, García- Borrón JC, Solano F. Molecular cloning and functional characterization of a unique multipotent polyphenol oxidase from Marinomonas mediterranea. Biochim Biophys Acta 2001; 157:104-16.
37. Durão P, Bento I, Fernandes AT, Melo EP, Lindley PF, Martins LO. Perturbations of the T1 copper site in the CotA laccase from Bacillus subtilis: structural, biochemical, enzymatic and stability studies. J Biol Inorg Chem 2006; 11:514-26.
38. Durão P, Chen Z, Silva CS, Soares CM, Pereira MM, Todorovic S, et al. Proximal mutations at the type 1 copper site of CotA laccase: spectroscopic, redox, kinetic and structural characterization of I494A and L386A mutants. Biochem J 2008; 412:339-46.
39. Pereira L, Coelho AV, Viegas CA, Santos MM, Robalo MP, Martins LO. Enzymatic biotransformation of the azo dye Sudan Orange G with bacterial CotA-laccase. J Biotechnol 2009; 139:68-77.
40. Endo K, Hayashi Y, Hibi T, Hosono K, Beppu T, Ueda K. Enzymological characterization of EpoA, a laccase-like phenol oxidase produced by Streptomyces griseus. J Biochem 2003; 133:671-7.
41. Ruijssenaars HJ, Hartmans S. A cloned Bacillus halodurans multicopper oxidase exhibiting alkaline laccase activity. Appl Microbiol Biotechnol 2004; 65:177-82.
42. Machczynski MC, Vijgenboom E, Samyn B, Canters GW. Characterization of SLAC: a small laccase from Streptomyces coelicolor with unprecedented activity. Protein Sci 2004; 13:2388-97.
43. Miyazaki K. A hyperthermophilic laccase from Thermus thermophilus HB27. Extremophiles 2005; 9:415-25.
44. Fernandes AT, Soares CM, Pereira MM, Huber R, Grass G, Martins LO. A robust metallo-oxidase from the hyperthermophilic bacterium Aquifex aeolicus. FEBS J 2007; 274:2683-94.
45. Koschorreck K, Richter SM, Ene AB, Roduner E, Schmid RD, Urlacher VB. Cloning and characterization of a new laccase from Bacillus licheniformis catalyzing dimerization of phenolic acids. Appl Microbiol Biotechnol 2008; 79:217-24.
46. Koschorreck K, Schmid RD, Urlacher VB. Improving the functional expression of a Bacillus licheniformis laccase by random and site-directed mutagenesis. BMC
47. Wang GD, Li QJ, Luo B, Chen XY. Ex planta phytoremedia-tion of trichlorophenol and phenolic allelo chemicals via an engineered secretory laccase. Nat Biotechnol 2004; 22:893-7.
48. Sonoki T, Kajita S, Ikeda S, Uesugi M, Tatsumi K, Katayama Y, et al. Transgenic tobacco expressing fungal laccase promotes the detoxification of environmental pollutants. Appl Microbiol Biotechnol 2005; 67:138-42.
49. Li L, Steffens JC. Overexpression of polyphenol oxidase in transgenic tomato plants results in enhanced bacterial disease resistance. Planta 2002; 215:239-47.
50. Bailey MR, Woodard SL, Callaway E, Beifuss K, Magallanes-Lundback M, Lane JR, Horn ME, et al. Improved recovery of active recombinant laccase from maize seed. Appl Microbiol Biotechnol 2004; 63:390-7.
51. LaFayette PR, Eriksson KE, Dean JF. Characterization and heterologous expression of laccase cDNAs from xylem tissues of yellow-poplar (Liriodendron tulipifera). Plant Mol Biol 1999; 40:23-35.
52. Sakamoto Y, Nakade K, Yano A, Nakagawa Y, Hirano T, Irie T, et al. Heterologous expression of lcc1 from Lentinula edodes in tobacco BY-2 cells results in the production an active, secreted form of fungal laccase. Appl Microbiol Biotechnol 2008; 79:971-80.
53. Nakagawa Y, Sakamoto Y, Kikuchi S, Sato T, Yano A. A chimeric laccase with hybrid properties of the parental Lentinula edodes laccases. Microbiol Res 2009; doi:10.1016/j.micres.2009.08.006.
54. de Wilde C, Uzan E, Zhou Z, Kruus K, Andberg M, Buchert J, et al. Transgenic rice as a novel production system for Melanocarpus and Pycnoporus laccases. Transgenic Res 2008; 17:515-27.
55. Dittmer NT, Gorman MJ, Kanost MR. Characterization of endogenous and recombinant forms of laccase-2, a multicopper oxidase from the tobacco hornworm, Manduca sexta. Insect Biochem Mol Biol 2009; 39:596-606.
56. Gellissen G, Hollenberg CP. Application of yeasts in gene expression studies: a comparison of Saccharomyces cerevisiae, Hansenula polymorpha and Kluyveromyces lactis: a review. Gene 1997; 190:87-97.
57. Sato Y, Wuli B, Sederoff R, Whetten R. Molecular cloning and expression of eight cDNAs in loblolly pine (Pinus taeda). J Plant Res 2001; 114:147-55.
58. Otterbein L, Record E, Longhi S, Asther M, Moukha S. Molecular cloning of the cDNA encoding laccase from Pycnoporus cinnabarinus I-937 and expression in Pichia pastoris. Eur J Biochem 2000; 267:1619-25.
59. Colao MC, Lupino S, Garzillo AM, Buonocore V, Ruzzi M. Heterologous expression of lccl gene from Trametes trogii in Pichia pastoris and characterization of the recombinant enzyme. Microb Cell Fact 2006; 5:31-6.
60. Piscitelli A, Giardina P, Mazzoni C, Sannia G. Recombinant expression of Pleurotus ostreatus laccases in Kluyveromyces lactis and Saccharomyces cerevisiae. Appl Microbiol Biotechnol 2005; 69:428-39.
61. Jolivalt C, Madzak C, Brault A, Caminade E, Malosse C, Mougin C. Expression of laccase IIIb from the white-rot fungus Trametes versicolor in the yeast Yarrowia lipolytica for environmental applications. Appl Microbiol Biotechnol 2005; 66:450-6.
62. Guo M, Lu FP, Pu J, Bai DQ, Du LX. Molecular cloning of the cDNA encoding laccase from Trametes versicolor and heterologous expression in Pichia methanolica. Appl Microbiol Biotechnol 2005; 69:178-83.
63. Kojima Y, Tsukuda Y, Kawai Y, Tsukamoto A, Sugiura J, Sakaino M, et al. Cloning, sequence analysis, and expression of ligninolytic phenoloxidase genes of the white-rot basidiomycete Coriolus hirsutus. J Biol Chem 1990; 265:15224-30.
64. Cassland P, Jonsson LJ. Characterization of a gene encoding Trametes versicolor laccase A and improved heterologous expression in Saccharomyces cerevisiae by decreased cultivation temperature. Appl Microbiol Biotechnol 1999; 52:393-400.
65. Larsson S, Cassland P, Jonsson LJ. Development of a Saccharomyces cerevisiae strain with enhanced resistance to phenolic fermentation inhibitors in lignocellulose hydrolysates by heterologous expression of laccase. Appl Environ Microbiol 2001; 67:1163-70.
66. Hoshida H, Nakao M, Kanazawa H, Kubo K, Hakukawa T, Morimasa K, et al. Isolation of five laccase gene sequences from the white-rot fungus Trametes sanguinea by PCR, and cloning, characterization and expression of the laccase cDNA in yeasts. J Biosci Bioeng 2001; 92:372-80.
67. Bulter T, Alcalde M, Sieber V, Meinhold P, Schlachtbauer C, Arnold FH. Functional expression of a fungal laccase in Saccharomyces cerevisiae by directed evolution. Appl Environ Microbiol 2003; 69:987-95.
68. Alcalde M, Bulter T, Zumárraga M, García-Arellano H, Mencía M, Plou FJ, et al. Screening mutant libraries of fungal laccases in the presence of organic solvents. J Biomol Screen 2005; 10:624-31.
69. Alcalde M, Zumárraga M, Polaina J, Ballesteros A, Plou FJ. Combinatorial saturation mutagenesis by in vivo overlap extension for the engineering of fungal laccases. Comb Chem High T Scr 2006; 9:719-27.
70. Zumárraga M, Bulter T, Shleev S, Polaina J, Martinez- Arias A, Plou FJ, et al. In vitro evolution of a fungal laccase in high concentrations of organic cosolvents. Chem Biol 2007; 14:1052-64.
71. Zumárraga M, Vaz Domínguez C, Camarero S, Shleev S, Polaina J, Martínez-Arias A, et al. Combinatorial saturation mutagenesis of the Myceliophthora thermophila laccase T2 mutant: the connection between the C-terminal plug and the conserved 509VSG511tripeptide. Comb Chem High-Throughput Screen 2008; 11:807-16.
72. Zumárraga M, Camarero S, Shleev S, Martínez-Arias A, Ballesteros A, Plou FJ, et al. Altering the laccase functionality by in vivo assembly of mutant libraries with different mutational spectra. Proteins 2008; 71:250-60.
73. Uldschmid A, Dombi R, Marbach K. Identification and functional expression of ctaA, a P-type ATPase gene involved in copper trafficking in Trametes versicolor. Microbiology 2003; 149:2039-48.
74. Kiiskinen LL, Saloheimo M. Molecular cloning and expression in Saccharomyces cerevisiae of a laccase gene from the ascomycete Melanocarpus albomyces. Appl Environ Microbiol 2004; 70:137-44.
75. Andberg M, Hakulinen N, Auer S, Saloheimo M, Koivula A, Rouvinen J, et al. Essential role of the C-terminus in Melanocarpus albomyces laccase for enzyme production, catalytic properties and structure. FEBS J 2009; 276:6285-300.
76. Klonowska A, Gaudin C, Asso M, Fournel A, Reglier M, Tron T. LAC3, a new low redox potential laccase from Trametes sp. strain C30 obtained as a recombinant protein in yeast. Enzyme Microb Technol 2005; 36:34-41.
77. Balland V, Hureau C, Cusano AM, Liu Y, Tron T, Limoges B. Oriented immobilization of a fully active monolayer of histidine-tagged recombinant laccase on modified gold electrodes. Chemistry 2008; 14:7186-92.
78. Cusano AM, Mekmouche Y, Meglecz E, Tron T. Plasticity of laccase generated by homeologous recombination in yeast. FEBS J 2009; 276:5471-80.
79. Festa G, Autore F, Fraternali F, Giardina P, Sannia G. Development of new laccases by directed evolution: functional and computational analyses. Proteins 2008; 72:25-34.
80. Miele A, Giardina P, Sannia G, Faraco V. Random mutants of a Pleurotus ostreatus laccase as new biocatalysts for industrial effluents bioremediation. J Appl Microbiol 2009; 108:998-1006.
81. Miele A, Faraco V, Piscitelli A, Del Vecchio C, Giardina P, Sannia G. Selection of 'better performing' laccases through directed evolution. FEBS J 2009; 276:377-8.
82. Autore F, Del Vecchio C, Fraternali F, Giardina P, Sannia G, Faraco V. Molecular determinants of peculiar properties of a Pleurotus ostreatus laccase: analysis by site-directed mutagenesis. Enzyme Microb Technol 2009; 45:507-13.
83. Hoshida H, Fujita T, Murata K, Kubo K, Akada R. Copper-dependent production of a Pycnoporus coccineus extracellular laccase in Aspergillus oryzae and Saccharomyces cerevisiae. Biosci Biotechnol Biochem 2005; 69:1090-7.
84. Necochea R, Valderrama B, Díaz-Sandoval S, Folch- Mallol JL, Vázquez-Duhalt R, Iturriaga G. Phylogenetic and biochemical characterisation of a recombinant laccase from Trametes versicolor. FEMS Microbiol Lett 2005; 244:235-41.
85. Kim D, Kwak E, Choi HT. Increase of yeast survival under oxidative stress by the expression of the laccase gene from Coprinellus congregatus. J Microbiol 2006; 44:617-21.
86. Bleve G, Lezzi C, Mita G, Rampino P, Perrotta C, Villanova L, et al. Molecular cloning and heterologous expression of a laccase gene from Pleurotus eryngii in free and immobilized Saccharomyces cerevisiae cells. Appl Microbiol Biotechnol 2008; 79:731-41.
87. Pezzella C, Autore F, Giardina P, Piscitelli A, Sannia G, Faraco V. The Pleurotus ostreatus laccase multi-gene family: isolation and heterologous expression of new family members. Curr Genet 2009; 55:45-57.
88. Jönsson LJ, Saloheimo M, Penttila M. Laccase from the white-rot fungus Trametes versicolor: cDNA cloning of lcc1 and expression in Pichia pastoris. Curr Genet 1997; 32:425-30.
89. O'Callaghan J, O'Brien MM, McClean K, Dobson ADW. Optimisation of the expression of a Trametes versicolor laccase gene in Pichia pastoris. J Ind Microbiol Biotechnol 2002; 29:55-9.
90. Hong F, Meinander NQ, Jönsson LJ. Fermentation strategies for improved heterologous expression of laccase in Pichia pastoris. Biotechnol Bioeng 2002; 79:438-49.
91. Williamson PR, Wakamatsu K, Ito S. Melanin biosynthesis in Cryptococcus neoformans. J Bacteriol 1998; 180:1570-2.
92. Gelo-Pujic M, Kim HH, Butlin NG, Palmore GT. Electrochemical studies of a truncated laccase produced in Pichia pastoris. Appl Environ Microbiol 1999; 65:5515-21.
93. Brown MA, Zhao Z, Mauk AG. Expression and characterization of a recombinant multi-copper oxidase: laccase IV from Trametes versicolor. Inorg Chim Acta 2002; 331:232-8.
94. Soden DM, O'Callaghan J, Dobson AD. Molecular cloning of a laccase isozyme gene from Pleurotus sajor-caju and expression in the heterologous Pichia pastoris host. Microbiology 2002; 148:4003-14.
95. Yang JQ, Tang GY, Dai HM, Liu G, Zhu HC. Expression and purification of the modified gene of laccase from Panus rudis in Pichia pastoris. Lett Biotechnol 2003; 16:255-8.
96. Liu W, Chao Y, Liu S, Bao H, Qian S. Molecular cloning and characterization of a laccase gene from the basidiomycete Fome lignosus and expression in Pichia pastoris. Appl Microbiol Biotechnol 2003; 63:174-81.
97. Hu MR, Chao YP, Zhang GQ, Yang XQ, Xue ZQ, Qian SJ. Molecular evolution of Fome lignosus laccase by ethyl methane sulfonate-based random mutagenesis in vitro. Biomol Eng 2007; 24:619-24.
98. Zhang YB, Jiang Q, Jiang ML, Ma LX. Cloning of a laccase gene from Flammulina velutipes and study on its expression in Pichia pastoris. Acta microbiologica Sinica 2004; 44:775-9.
99. Zhang YB, Jiang ML, Hu XJ, Zhang GM, Ma LX. Expression of a laccase gene from Pleurotus ostreatus in Pichia pastoris and characterization of the recombinant enzyme. Acta microbiologica Sinica 2005; 45:625-9.
100. Bohlin C, Jönsson LJ, Roth R, van Zyl WH. Heterologous expression of Trametes versicolor laccase in Pichia pastoris and Aspergillus niger. Appl Biochem Biotechnol 2006; 129:195-214.
101. Hong Y, Xiao Y, Zhou H, Fang W, Zhang M, Wang J, et al. Expression of a laccase cDNA from Trametes sp. AH28-2 in Pichia pastoris and mutagenesis of transformants by nitrogen ion implantation. FEMS Microbiol Lett 2006; 258:96-101.
102. Li F, Hong YZ, Xiao YZ, Xu YH, Fang W. High production of laccase B from Trametes sp. in Pichia pastoris. World J Microbiol Biotechnol 2007; 23:741-5.
103. Hong YZ, Zhou HM, Tu XM, Li JF, Xiao YZ. Cloning of a laccase gene from a novel basidiomycete Trametes sp 420 and its heterologous expression in Pichia pastoris. Curr Microbiol 2007; 54:260-5.
104. Cui TJ, Wang XT, Zhou HM, Hong YZ, Xiao YZ, Cui TJ, et al. High output of a Trametes laccase in Pichia pastoris and characterization of recombinant enzymes. Sheng Wu Gong Cheng Xue Bao 2007; 23:1055-9.
105. Zhou HM, Hong YZ, Xiao YZ, Cui TJ, Wang XT, Pu CL. High output of a Trametes laccase in Pichia pastoris and characterization of recombinant enzymes. Chin J Biotech 2007; 23:1055-9.
106. Xu Y, Hong Y, Xiao Y, Fang W. Preparation and application of polyclonal antibody against a recombinant laccase. Cell Mol Immunol 2007; 4:315-7.
107. Li JF, Hong YZ, Xiao YZ. Cloning and heterologous expression of the gene of laccase C from Trametes sp. 420 and potential of recombinant laccase in dye decolorization. Acta microbiologica Sinica 2007; 47:54-8.
108. Joo SS, Ryu IW, Park JK, Yoo YM, Lee DH, Hwang KW, et al. Molecular cloning and expression of a laccase from Ganoderma lucidum, and its antioxidative properties. Mol Cells 2008; 25:112-8.
109. Lu L, Zhao M, Liang SC, Zhao LY, Li DB, Zhang BB. Production and synthetic dyes decolourization capacity of a recombinant laccase from Pichia pastoris. J Appl Microbiol 2009; 107:1149-56.
110. Colao MC, Caporale C, Silvestri F, Ruzzi M, Buonocore V. Modeling the 3-D structure of a recombinant laccase from Trametes trogii active at a pH close to neutrality. Protein J 2009; 28:375-83.
111. Camattari A, Bianchi MM, Branduardi P, Porro D, Brambilla L. Induction by hypoxia of heterologous-protein production with the KlPDC1 promoter in yeasts. Appl Environ Microbiol 2007; 73:922-9.
112. Ranieri D, Colao MC, Ruzzi M, Romagnoli G, Bianchi MM. Optimization of recombinant fungal laccase production with strains of the yeast Kluyveromyces lactis from the pyruvate decarboxylase promoter. FEMS Yeast Res 2009; 9:892-902.
113. Faraco V, Ercole C, Festa G, Giardina P, Piscitelli A, Sannia G. Heterologous expression of heterodimeric laccase from Pleurotus ostreatus in Kluyveromyces lactis. Appl Microbiol Biotechnol 2008; 77:1329-35.
114. Madzak C, Mimmi MC, Caminade E, Brault A, Baumberger S, Briozzo P, et al. Shifting the optimal pH of activity for a laccase from the fungus Trametes versicolor by structure-based mutagenesis. Protein Eng Des Sel 2006; 19:77-84.
115. Madzak C, Otterbein L, Chamkha M, Moukha S, Asther M, Gaillardin C, et al. Heterologous production of a laccase from the basidiomycete Pycnoporus cinnabarinus in the dimorphic yeast Yarrowia lipolytica. FEMS Yeast Res 2005; 5:635-46.
116. Guo M, Lu FP, Du LX, Pu J, Bai DQ. Optimization of the expression of a laccase gene from Trametes versicolor in Pichia methanolica. Appl Microbiol Biotechnol 2006; 71:848-52.
117. Guo M, Lu F, Liu M, Li T, Pu J, Wang N, et al. Purification of recombinant laccase from Trametes versicolor in Pichia methanolica and its use for the decolorization of anthraquinone dye. Biotechnol Lett 2008; 30:2091-6.
118. Nevalainen KM, Te'o VS, Bergquist PL. Heterologous protein expression in filamentous fungi. Trends Biotechnol 2005; 23:468-74.
119. Saloheimo M, Niku-Paavola ML. Heterologous production of a ligninolytic enzyme: expression of the Phlebia radiata laccase gene in Trichoderma reesei. Bio Technology 1991; 9:987-90.
120. Baker CJO, White TC. Expression of the laccase IV gene from Trametes versicolor in Trichoderma reesei. Abst Papers Am Chem Soc 2000; 219:154.
121. Kiiskinen LL, Kruus K, Bailey M, Ylosmaki E, Siika- Aho M, Saloheimo M. Expression of Melanocarpus albomyces laccase in Trichoderma reesei and characterisation of the purified enzyme. Microbiol 2004; 150:3065-74.
122. Kiiskinen LL, Palonen H, Linder M, Viikari L, Kruus K. Laccase from Melanocarpus albomyces binds effectively to cellulose. FEBS Lett 2004; 576:251-5.
123. Hakulinen N, Kruus K, Koivula A, Rouvinen J. A crystallographic and spectroscopic study on the effect of X-ray radiation on the crystal structure of Melanocarpus albomyces laccase. Biochem Biophys Res Commun 2006; 350:929-34.
124. Rautio JJ, Smit BA, Wiebe M, Penttilä M, Saloheimo M. Transcriptional monitoring of steady state and effects of anaerobic phases in chemostat cultures of the filamentous fungus Trichoderma reesei. BMC Genomics 2006; 7:247.
125. Rautio JJ, Bailey M, Kivioja T, Söderlund H, Penttilä M, Saloheimo M. Physiological evaluation of the filamentous fungus Trichoderma reesei in production processes by marker gene expression analysis. BMC Biotechnol 2007; 7:28.
126. Hakulinen N, Andberg M, Kallio J, Koivula A, Kruus K, Rouvinen J. A near atomic resolution structure of a Melanocarpus albomyces laccase. J Struct Biol 2008; 162:29-39.
127. Wahleithner JA, Xu F, Brown SH, Golightly EJ, Halkier T, Kauppinen S, et al. The identification and characterization of four laccases from the plant pathogenic fungus Rhizoctonia solani. Curr Genet 1996; 29:395-403.
128. Xu F, Shin W, Brown SH, Wahleithner JA, Sundaram UM, Solomon WI. A study of a series of recombinant fungal laccases and bilirubin oxidase that exhibit significant differences in redox potential, substrate specificity, and stability. Biochim Biophys Acta 1996; 1292:303-11.
129. Xu F, Berka RM, Wahleithner JA, Nelson BA, Shuster JR, Brown SH, et al. Site-directed mutations in fungal laccase: effect on redox potential, activity and pH profile. Biochem J 1998; 334:63-70.
130. Kulys J, Bratkovskaja I, Vidziunaite R. Laccase-catalysed iodide oxidation in presence of methyl syringate. Biotechnol Bioeng 2005; 92:124-8.
131. Yaver DS, Xu F, Golightly EJ, Brown KM, Brown SH, Rey MW, et al. Purification, characterization, molecular cloning, and expression of two laccase genes from the white rot basidiomycete Trametes villosa. Appl Environ Microbiol 1996; 62:834-41.
132. Xu F, Palmer AE, Yaver DS, Berka RM, Gambetta GA, Brown SH, et al. Targeted mutations in a Trametes villosa laccase, axial perturbations of the T1 copper. J Biol Chem 1999; 274:12372-5.
133. Kulys J, Krikstopaitis K, Ziemys A, Schneider PP. Laccase-catalysed oxidation of syringates in presence of albumins. J Mol Cat B Enzym 2002; 18:99-108.
134. Bukh C, Lund M, Bjerrum MJ. Kinetic studies on the reaction between Trametes villosa laccase and dioxygen. J Inorg Biochem 2006; 100:1547-57.
135. Kulys J, Bratkovskaja I. Antioxidants determination with laccase. Talanta 2007; 72:526-31.
136. Berka R, Schneider P, Golightly E, Brown S, Madden M, Brown K, et al. Characterization of the gene encoding an extracellular laccase of Myceliophthora thermophila and analysis of the recombinant enzyme expressed in Aspergillus oryzae. Appl Environ Microbiol 1997; 63:3151-7.
137. López-Cruz JI, Viniegra-Gonzalez G, Hernández- Arana A. Thermostability of native and pegylated Myceliophthora thermophila laccase in aqueous and mixed solvents. Bioconjug Chem 2006; 17:1093-8.
138. Yaver DS, Overjero MDC, Xu F, Nelson BA, Brown KM, Halkier T, et al. Molecular characterization of laccase genes from the basidiomycete Coprinus cinereus and heterologous expression of the laccase Lcc1. Appl Environ Microbiol 1999; 65:4943-8.
139. Ducros V, Brzozowski AM, Wilson KS, Brown SH, Ostergaard P, Schneider P, et al. Crystal structure of the type-2 Cu depleted laccase from Coprinus cinereus at 2.2 angstrom resolution. Nat Struct Biol 1998; 5:310-6.
140. Berka RM, Xu F, Thompson SA. 1995; I P WO 9533837 A1.
141. Sigoillot C, Record E, Belle V, Robert JL, Levasseur A, Punt PJ, et al. Natural and recombinant fungal laccases for paper pulp bleaching. Appl Microbiol Biotechnol 2004; 64:346-52.
142. Fujihiro S, Higuchi R, Hisamatsu S, Sonoki S. Metabolism of hydroxylated PCB congeners by cloned laccase isoforms. Appl Microbiol Biotechnol 2009; 82:853-60.
143. Yano A, Kikuchi S, Nakagawa Y, Sakamoto Y, Sato T. Secretory expression of the non-secretory-type Lentinula edodes laccase by Aspergillus oryzae. Microbiol Res 2009; 164:642-9.
144. Skálová T, Dohnálek J, stergaard LH, stergaard PR, Kolenko P, Dusková J, et al. The structure of the small laccase from Streptomyces coelicolor reveals a link between laccases and nitrite reductases. J Mol Biol 2009; 385:1165-78.
145. Hatamoto O, Sekine H, Nakano E, Abe K. Cloning and expression of a cDNA encoding the laccase from Schizophyllum commune. Biosci Biotechnol Biochem 1999; 63:58-64.
146. Record E, Punt PJ, Chamkha M, Labat M, van Den Hondel CA, Asther M. Expression of the Pycnoporus cinnabarinus laccase gene in Aspergillus niger and characterization of the recombinant enzyme. Eur J Biochem 2002; 269:602-9.
147. Sigoillot C, Camarero S, Vidal T, Record E, Asther M, Pérez-Boada M, et al. Comparison of different fungal enzymes for bleaching high-quality paper pulps. J Biotechnol 2005; 115:333-43.
148. Ravalason H, Herpoël-Gimbert I, Record E, Bertaud F, Grisel S, de Weert S, et al. Fusion of a family 1 carbohydrate binding module of Aspergillus niger to the Pycnoporus cinnabarinus laccase for efficient softwood kraft pulp biobleaching. J Biotechnol 2009; 142:220-6.
149. Aehle W, Convents D, Doornink M, Van Gastel F, Rodriguez AM, Toppozada A, et al. Detergent compositions comprising novel phenol oxidizing enzymes. 2002; WO 0220711.
150. Aehle W, Baldwin TL, Janssen GG, Van Gastel FJC, Wang H, Winetzky DS. Selection and production of laccase and other phenol oxidizing-binding peptide complexes for detergents and carotenoid stain removal. 2003; WO 2003023067.
151. Janssen GG, Baldwin TM, Winetzky DS, Tierney LM, Wang H, Murray CJ. Selective targeting of a laccase from Stachybotrys chartarum covalently linked to a carotenoid-binding peptide. J Pept Res 2004; 64:10-24.
152. Larrondo LF, Avila M, Salas L, Cullen D, Vicuna R. Heterologous expression of laccase cDNA from Ceriporiopsis subvermispora yields copper-activated apoprotein and complex isoform patterns. Microbiology 2003; 149:1177-82.
153. Valkonen M, Ward M, Wang H, Penttilä M, Saloheimo M. Improvement of foreign-protein production in Aspergillus niger var. awamori by constitutive induction of the unfolded-protein response. Appl Environ Microbiol 2003; 69:6979-86.
154. Wang Y, Xue W, Sims AH, Zhao C, Wang A, Tang G, et al. Isolation of four pepsin-like protease genes from Aspergillus niger and analysis of the effect of disruptions on heterologous laccase expression. Fungal Genet Biol 2008; 45:17-27.
155. Téllez-Jurado A, Arana-Cuenca A, González Becerra AE, Viniegra-González G, Loera O. Expression of a heterologous laccase by Aspergillus niger cultured by solid-state and submerged fermentations. Enzyme Microb Technol 2006; 38:665-9.
156. Weenink XO, Punt PJ, van den Hondel CA, Ram AF. A new method for screening and isolation of hypersecretion mutants in Aspergillus niger. Appl Microbiol Biotechnol 2006; 69:711-7.
157. Rodríguez E, Ruiz-Dueñas FJ, Kooistra R, Ram A, Martínez AT, Martínez MJ. Isolation of two laccase genes from the white-rot fungus Pleurotus eryngii and heterologous expression of the pel3 encoded protein. J Biotechnol 2008; 134:9-19.
158. Alberts JF, Gelderblom WC, Botha A, van Zyl WH. Degradation of aflatoxin B(1) by fungal laccase enzymes. Int J Food Microbiol 2009; 135:47-52.
159. Baldrian P. Fungal laccases-occurrence and properties. FEMS Microbiol Rev 2006; 30:215-42.
160. Palmieri G, Cennamo G, Sannia G. Remazol Brilliant Blue R decolourisation by the fungus Pleurotus ostreatus and its oxidative enzymatic system. Enzyme Microb Technol 2005; 36:17-24.
161. Koschorreck K, Richter SM, Swierczek A, Beifuss U, Schmid RD, Urlacher VB. Comparative characterization of four laccases from Trametes versicolor concerning phenolic C-C coupling and oxidation of PAHs. Arch Biochem Biophys 2008; 474:213-9.
162. Kunamneni A, Ballesteros A, Plou FJ, Alcalde M. Fungal laccase-a versatile enzyme for biotechnological applications. In: Mendez-Vilas A, ed. Communicating Current Research and Educational Topics and Trends in Applied Microbiology. Microbiology Book Series. Badajoz: Formatex 2007; 233-45.
163. De Quan, Kim Y, Yoon KB, Shin W. Assembly of Laccase over Platinum Oxide Surface and Application as an Amperometric Biosensor. Bull Korean Chem Soc 2002; 23:385-90.
164. De Quan, Shin W. Amperometric Detection of Catechol and Catecholamines by Immobilized Laccase from DeniLite. Electroanalysis 2004; 16:1576-82.
165. Franzoi AC, Dupont J, Spinelli A, Vieira IC. Biosensor based on laccase and an ionic liquid for determination of rosmarinic acid in plant extracts. Talanta 2009; 77:1322-7.
166. Franzoi AC, Migowski P, Dupont J, Vieira IC. Development of biosensors containing laccase and imidazolium bis(trifluoromethylsulfonyl)imide ionic liquid for the determination of rutin. Anal Chim Acta 2009; 639:90-5.
167. Franzoi AC, Vieira IC, Dupont J, Scheeren CW, de Oliveira LF. Biosensor for luteolin based on silver or gold nanoparticles in ionic liquid and laccase immobilized in chitosan modified with cyanuric chloride. Analyst 2009; 134:2320-8.
168. Cristóvão RO, Tavares AP, Loureiro JM, Boaventura RA, Macedo EA. Treatment and kinetic modelling of a simulated dye house effluent by enzymatic catalysis. Bioresour Technol 2009; 100:6236-42.