Molecular cloning and characterization of a novel metagenome-derived multicopper oxidase with alkaline laccase activity and highly soluble expression

Applied Microbiology and Biotechnology

Volumn 87, Issue 3, 2010, Pages 1023-1031

  Ye, Mao ^a   Li, Gang ^a   Liang, Wei Qu ^b   Liu, Yu Huan ^a  

^a SUN YAT SEN UNIVERSITY   (China)

^b Dongguan Agriculture Research Center   (China)

Author keywords

Alkaline laccase; Enzyme characterization; Gene cloning; Metagenome; Multicopper oxidase

Indexed keywords

ENZYME CHARACTERIZATION; GENE CLONING; LACCASES; METAGENOMES; MULTICOPPER OXIDASE;

ALCOHOLS; AMINO ACIDS; CLONING; ENZYMES; ESCHERICHIA COLI; GENE ENCODING; GENE EXPRESSION; INDUSTRIAL APPLICATIONS; ORGANIC ACIDS; PHENOLS; PURIFICATION; SOILS; SUBSTRATES;

ENZYME ACTIVITY;

ACETOSYRINGONE; ALKALINE LACCASE; BACTERIAL ENZYME; GUAIACOL; LACCASE; MULTICOPPER OXIDASE; OXIDOREDUCTASE; PROTEIN; PROTEIN LAC 591; RECOMBINANT ENZYME; UNCLASSIFIED DRUG; BACTERIAL PROTEIN; COPPER OXIDASE;

AMINO ACID; BIOCHEMISTRY; BIOTECHNOLOGY; COLIFORM BACTERIUM; ENZYME ACTIVITY; GENE EXPRESSION; GENOMICS; INDUSTRIAL TECHNOLOGY; MANGROVE; PROTEIN; SUBSTRATE;

ARTICLE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME SPECIFICITY; ENZYME SUBSTRATE; ESCHERICHIA COLI; GENE OVEREXPRESSION; METAGENOME; MOLECULAR CLONING; NONHUMAN; PROTEIN EXPRESSION; PROTEIN PURIFICATION; SEQUENCE ANALYSIS; AMINO ACID SEQUENCE; CHEMISTRY; ENZYME STABILITY; GENE EXPRESSION; GENETICS; KINETICS; METABOLISM; MICROBIOLOGY; MOLECULAR GENETICS; SEQUENCE ALIGNMENT; SOLUBILITY;

AMINO ACID SEQUENCE; BACTERIAL PROTEINS; CLONING, MOLECULAR; ENZYME STABILITY; GENE EXPRESSION; KINETICS; LACCASE; METAGENOME; MOLECULAR SEQUENCE DATA; OXIDOREDUCTASES; SEQUENCE ALIGNMENT; SOIL MICROBIOLOGY; SOLUBILITY; SUBSTRATE SPECIFICITY;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 77955653926     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-010-2507-5     Document Type: Article

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