Functional expression of a blood tolerant laccase in Pichia pastoris

BMC Biotechnology

Volumn 13, Issue , 2013, Pages 1-12

  Mate, Diana M ^a   Gonzalez Perez, David ^a   Kittl, Roman ^b   Ludwig, Roland ^b   Alcalde, Miguel ^a  

^a INSTITUTO DE CATÁLISIS Y PETROLEOQUÍMICA   (Spain)

^b UNIVERSITY OF NATURAL RESOURCES AND LIFE SCIENCES   (Austria)

Author keywords

Blood tolerance; Directed evolution; Functional expression; Halide inhibition; High redox potential laccase; Hydroxyl inhibition; Pichia pastoris; Saccharomyces cerevisiae

Indexed keywords

BLOOD; CLONING; PHYSIOLOGY; REDOX REACTIONS; SECONDARY BATTERIES; YEAST;

BIO-ELECTROCHEMICAL; DIRECTED EVOLUTION; FED-BATCH BIOREACTORS; FUNCTIONAL EXPRESSION; LACCASES; N-TERMINAL EXTENSION; PHYSIOLOGICAL FLUIDS; PICHIA PASTORIS;

ENZYMES;

HALIDE; LACCASE; RECOMBINANT ENZYME; HYBRID PROTEIN; SODIUM CHLORIDE; SODIUM FLUORIDE;

ARTICLE; ENZYME PURIFICATION; ENZYME RELEASE; ENZYME SYNTHESIS; FED BATCH REACTOR; GLYCOSYLATION; NONHUMAN; PH; PICHIA PASTORIS; PROMOTER REGION; PROTEIN EXPRESSION; SACCHAROMYCES CEREVISIAE; THERMOSTABILITY; BIOSYNTHESIS; CHEMICAL STRUCTURE; CHEMISTRY; DIRECTED MOLECULAR EVOLUTION; ENZYME STABILITY; FUNCTIONS OF THE HEMIC, LYMPHATIC AND RETICULOENDOTHELIAL SYSTEMS; GENETICS; HUMAN; KINETICS; METABOLISM; METHODOLOGY; MOLECULAR CLONING; MUTATION; PICHIA; PROTEIN ENGINEERING;

PICHIA PASTORIS; SACCHAROMYCES CEREVISIAE;

BLOOD PHYSIOLOGICAL PHENOMENA; CLONING, MOLECULAR; DIRECTED MOLECULAR EVOLUTION; ENZYME STABILITY; GLYCOSYLATION; HUMANS; KINETICS; LACCASE; MODELS, MOLECULAR; MUTATION; PICHIA; PROTEIN ENGINEERING; RECOMBINANT FUSION PROTEINS; SACCHAROMYCES CEREVISIAE; SODIUM CHLORIDE; SODIUM FLUORIDE;

EID: 84877834372     PISSN: None     EISSN: 14726750     Source Type: Journal    
DOI: 10.1186/1472-6750-13-38     Document Type: Article

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