Optimization of the expression of a laccase gene from Trametes versicolor in Pichia methanolica

Applied Microbiology and Biotechnology

Volumn 71, Issue 6, 2006, Pages 848-852

  Guo, Mei ^a,b   Lu, Fuping ^a   Du, Lianxiang ^a   Pu, Jun ^a   Bai, Dongqing ^a,b  

^a TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

^b TIANJIN AGRICULTURAL UNIVERSITY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

COPPER; DNA; ENZYME KINETICS; FUNGI; METHANOL; PH EFFECTS; PROTEINS;

LACCASE (LCC1); PICHIA METHANOLICA; TRAMETES VERSICOLOR;

GENES;

COMPLEMENTARY DNA; COPPER; LACCASE; METHANOL; RECOMBINANT PROTEIN;

COPPER; DNA; ENZYME ACTIVITY; FUNGUS; GENE EXPRESSION; OPTIMIZATION; POLYMERASE CHAIN REACTION; YEAST;

ARTICLE; DNA ISOLATION; ENZYME ACTIVITY; FUNGAL STRAIN; FUNGUS; GENE ACTIVITY; GENE EXPRESSION; IMMUNODETECTION; MOLECULAR CLONING; MOLECULAR WEIGHT; NONHUMAN; PH; PICHIA; PICHIA METHANOLICA; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; TEMPERATURE; TRAMETES VERSICOLOR; WESTERN BLOTTING;

BLOTTING, WESTERN; CLONING, MOLECULAR; COPPER; DNA, COMPLEMENTARY; DNA, FUNGAL; ELECTROPHORESIS, POLYACRYLAMIDE GEL; FUNGAL PROTEINS; GENE EXPRESSION REGULATION, ENZYMOLOGIC; GENE EXPRESSION REGULATION, FUNGAL; HYDROGEN-ION CONCENTRATION; LACCASE; METHANOL; PICHIA; POLYPORALES; TEMPERATURE;

FUNGI; PICHIA METHANOLICA; TRAMETES VERSICOLOR;

EID: 33746750905     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-005-0210-8     Document Type: Article

References (10)

1. Cassland P, Jönsson LJ (1999) Characterization of a gene encoding Trametes versicolor laccase A and improved heterologous expression in Saccharomyces cerevisiae by decreased cultivation temperature. Appl Microbiol Biotechnol 52:393-400
2. Choi J, Kim EE (2002) Expression of the active human and duck hepatitis B virus polymerases in heterologous system of Pichia methanolica. Antivir Res 55:279-290
3. Fahraeus G, Reinhammar B (1967) Large scale production and purification of laccase from the fungus Polyporus versicolor and some properties of laccase. Acta Chem Scand 21:2367-2378
4. Gianfreda L, Xu F and Bollag JM (1999) Laccase: a useful group of oxidoreductive enzymes. Biores J 3:1-25
5. Jönsson L, Saloheimo M, Penttila M (1997) Laccase from the white-rot fungus Trametes versicolor: cDNA cloning of lcc1 and expression in Pichia pastoris. Curr Genet 32:425-430
6. Kojima Y, Tsukuda Y, Kawai Y, Tsukamoto A, Sugiura J, Sakaino M, Kita Y (1990) Cloning, sequence analysis, and expression of ligninolytic phenoloxidase genes of the white-rot basidiomycete Coriolus hirsutus. J Biol Chem 265:15224-15230
7. Messerschmidt A (1993) Blue copper oxidases. Adv Inorg Chem 40:121-185
8. Roy-Arcand L, Archibald FS (1991) Direct dechlorination of chlorophenolic compounds by laccases from Trametes (Coriolus) versicolor. Enzyme Microb Technol 13:194-203
9. Thurston CF (1994) The structure and function of fungal laccase. Microbiology 140:19-26
10. Yaver DS, Xu F, Golightly EJ, Brown SH, Rey MW, Schneider P, Halkier T, Mondorf K, Dallboge H (1996) Purification, characterization, molecular cloning and expression of two laccases from the white rot basidiomycete Trametes villosa. Appl Environ Microbiol 62:834-841