Development of chimeric laccases by directed evolution

Biotechnology and Bioengineering

Volumn 109, Issue 12, 2012, Pages 2978-2986

  Pardo, Isabel ^a   Vicente, Ana Isabel ^a   Mate, Diana M ^b   Alcalde, Miguel ^b   Camarero, Susana ^a  

^a CENTRO DE INVESTIGACIONES BIOLÓGICAS   (Spain)

^b INSTITUTO DE CATÁLISIS Y PETROLEOQUÍMICA   (Spain)

Author keywords

factor prepro leader; Chimeric laccases; DNA shuffling; High throughput screening; S. cerevisiae

Indexed keywords

CROSSOVER EVENTS; DIRECTED EVOLUTION; DNA RECOMBINATION; DNA SHUFFLING; FUNCTIONAL EXPRESSION; FUNGAL LACCASES; FUSION GENES; HIGH-THROUGHPUT SCREENING; IN-VITRO; LACCASES; PROTEIN ENGINEERING; PYCNOPORUS CINNABARINUS; S.CEREVISIAE;

DNA; GENETIC ENGINEERING; REDOX REACTIONS;

YEAST;

CHIMERIC PROTEIN; DNA; LACCASE;

ARTICLE; CHIMERA; CULTURE MEDIUM; DNA SHUFFLING; FUNGUS CULTURE; HIGH THROUGHPUT SCREENING; NONHUMAN; PH; POLYMERASE CHAIN REACTION; SACCHAROMYCES CEREVISIAE; THERMOSTABILITY;

BASIDIOMYCOTA; BENZOTHIAZOLES; DIRECTED MOLECULAR EVOLUTION; ENZYME STABILITY; FUNGAL PROTEINS; GENE LIBRARY; LACCASE; MODELS, GENETIC; MODELS, MOLECULAR; PROTEIN ENGINEERING; PYROGALLOL; RECOMBINANT FUSION PROTEINS; SACCHAROMYCES CEREVISIAE; SEQUENCE ALIGNMENT; SULFONIC ACIDS;

PYCNOPORUS CINNABARINUS; SACCHAROMYCES CEREVISIAE;

EID: 84868106496     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.24588     Document Type: Article

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