Engineering platforms for directed evolution of laccase from Pycnoporus cinnabarinus

Applied and Environmental Microbiology

Volumn 78, Issue 5, 2012, Pages 1370-1384

  Camarero, S ^a,b   Pardo, I ^b   Canas A I ^a,b   Molina, P ^a,b   Record, E ^c   Martinez A T ^b   Martinez M J ^b   Alcalde, M ^a  

^a INSTITUTO DE CATÁLISIS Y PETROLEOQUÍMICA   (Spain)

^b CENTRO DE INVESTIGACIONES BIOLÓGICAS   (Spain)

^c AIX MARSEILLE UNIVERSITY   (France)

Author keywords

[No Author keywords available]

Indexed keywords

ACTIVITY PROFILE; ASPERGILLUS NIGER; BIOCATALYSIS; DIRECTED EVOLUTION; FUNCTIONAL EXPRESSION; FUNGAL LACCASES; FUSION GENES; HETEROLOGOUS EXPRESSION; LACCASES; PROTEIN ENGINEERING; PYCNOPORUS CINNABARINUS; REDOX MEDIATORS; S.CEREVISIAE; SIGNAL PEPTIDE; SUBSTRATE SPECIFICITY;

ASPERGILLUS; GENE EXPRESSION; GENETIC ENGINEERING; PHENOLS; SUBSTRATES; YEAST;

BIOCHEMICAL ENGINEERING;

LACCASE; RECOMBINANT PROTEIN; SIGNAL PEPTIDE;

BIOENGINEERING; CATALYSIS; GENE; MUTATION; OPTIMIZATION; OXIDATION; PH; PROTEIN; REDOX POTENTIAL; SUBSTRATE; YEAST;

ARTICLE; ASPERGILLUS NIGER; CHEMICAL STRUCTURE; DIRECTED MOLECULAR EVOLUTION; ENZYME SPECIFICITY; ENZYMOLOGY; GENETICS; KINETICS; MASS SCREENING; METABOLISM; METHODOLOGY; MUTATION; NUCLEOTIDE SEQUENCE; OXIDATION REDUCTION REACTION; PH; PROTEIN TERTIARY STRUCTURE; PYCNOPORUS; SACCHAROMYCES CEREVISIAE; SECRETION;

ASPERGILLUS NIGER; DIRECTED MOLECULAR EVOLUTION; DNA MUTATIONAL ANALYSIS; HYDROGEN-ION CONCENTRATION; KINETICS; LACCASE; MASS SCREENING; MODELS, MOLECULAR; MUTATION; OXIDATION-REDUCTION; PROTEIN SORTING SIGNALS; PROTEIN STRUCTURE, TERTIARY; PYCNOPORUS; RECOMBINANT PROTEINS; SACCHAROMYCES CEREVISIAE; SUBSTRATE SPECIFICITY;

ASPERGILLUS NIGER; PYCNOPORUS CINNABARINUS; SACCHAROMYCES CEREVISIAE;

EID: 84857093916     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.07530-11     Document Type: Article

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