Directed evolution of CotA laccase for increased substrate specificity using Bacillus subtilis spores

Protein Engineering, Design and Selection

Volumn 23, Issue 8, 2010, Pages 679-682

  Gupta, Nirupama ^a   Farinas, Edgardo T ^a  

^a NEW JERSEY INSTITUTE OF TECHNOLOGY   (United States)

Author keywords

directed evolution; laccase; protein display; protein engineering; substrate specificity

Indexed keywords

ALDEHYDES; BACTERIA; BACTERIOLOGY; BIOCHEMICAL ENGINEERING; BIOLOGY; GENETIC ENGINEERING; LIBRARIES; SUBSTRATES;

DIRECTED EVOLUTION; LACCASE; LACCASES; PROTEIN ENGINEERING; SUBSTRATE SPECIFICITY;

PROTEINS;

2,2' AZINOBIS(3 ETHYLBENZOTHIAZOLINE 6 SULFONIC ACID); 4 HYDROXY 3,5 DIMETHOXY BENZALDEHYDE AZINE; LACCASE; PIPERAZINE DERIVATIVE; PROTEIN COT A; UNCLASSIFIED DRUG; 2,2'-AZINO-DI-(3-ETHYLBENZOTHIAZOLINE)-6-SULFONIC ACID; BACTERIAL PROTEIN; BENZOTHIAZOLE DERIVATIVE; IMMOBILIZED ENZYME; PEPTIDE LIBRARY; SULFONIC ACID DERIVATIVE;

ARTICLE; BACILLUS SUBTILIS; BACTERIOPHAGE; CELL SURFACE; ENZYME ENGINEERING; ENZYME SPECIFICITY; GENE ACTIVITY; GENE EXPRESSION; GENE LIBRARY; GENETIC SCREENING; GRAM NEGATIVE BACTERIUM; MOLECULAR LIBRARY; NONHUMAN; PHYSICAL CHEMISTRY; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN IMMOBILIZATION; PROTEIN STABILITY; YEAST; BACTERIAL SPORE; BINDING SITE; CHEMICAL STRUCTURE; CHEMISTRY; DIRECTED MOLECULAR EVOLUTION; ENZYMOLOGY; GENETICS; KINETICS; METABOLISM; PEPTIDE LIBRARY; PROCEDURES; REPRODUCIBILITY; SITE DIRECTED MUTAGENESIS;

BACILLUS SUBTILIS; BACTERIAL PROTEINS; BENZOTHIAZOLES; BINDING SITES; DIRECTED MOLECULAR EVOLUTION; ENZYMES, IMMOBILIZED; KINETICS; LACCASE; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; PEPTIDE LIBRARY; REPRODUCIBILITY OF RESULTS; SPORES, BACTERIAL; SUBSTRATE SPECIFICITY; SULFONIC ACIDS;

EID: 77954994517     PISSN: 17410126     EISSN: 17410134     Source Type: Journal    
DOI: 10.1093/protein/gzq036     Document Type: Article

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