Lessons from diversity of directed evolution experiments by an analysis of 3,000 mutations

Biotechnology and Bioengineering

Volumn 111, Issue 12, 2014, Pages 2380-2389

  Zhao, Jing ^a   Kardashliev, Tsvetan ^a   Joëlle Ruff, Anna ^a   Bocola, Marco ^a   Schwaneberg, Ulrich ^a  

^a RWTH AACHEN UNIVERSITY   (Germany)

Author keywords

Directed evolution; EpPCR; Polymerase bias; Protein engineering; Random mutagenesis; SeSaM

Indexed keywords

AMINO ACIDS; CLONING; LIBRARIES; MUTAGENESIS; PROTEINS;

DIRECTED EVOLUTION; EPPCR; POLYMERASE BIAS; PROTEIN ENGINEERING; RANDOM MUTAGENESIS; SESAM;

EXPERIMENTS;

AMINO ACID SUBSTITUTION; ARTICLE; BACILLUS SUBTILIS; CODON; DIRECTED MOLECULAR EVOLUTION; ERROR PRONE POLYMERASE CHAIN REACTION; GENE LIBRARY; GENE MUTATION; MUTAGENESIS; MUTATION RATE; MUTATIONAL ANALYSIS; NONHUMAN; POLYMERASE CHAIN REACTION; PROTEIN ENGINEERING; BIOLOGICAL MODEL; DNA SEQUENCE; GENETICS; MUTATION; PROCEDURES; STATISTICAL MODEL;

AMINO ACID SUBSTITUTION; DIRECTED MOLECULAR EVOLUTION; MODELS, GENETIC; MODELS, STATISTICAL; MUTAGENESIS; MUTATION; POLYMERASE CHAIN REACTION; PROTEIN ENGINEERING; SEQUENCE ANALYSIS, DNA;

EID: 84918832461     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.25302     Document Type: Article

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