Further improvement of phosphite dehydrogenase thermostability by saturation mutagenesis

Biotechnology and Bioengineering

Volumn 99, Issue 2, 2008, Pages 268-274

  McLachlan, Michael J ^a   Johannes, Tyler W ^a   Zhao, Huimin ^a  

^a UNIVERSITY OF ILLINOIS AT URBANA CHAMPAIGN   (United States)

Author keywords

Directed evolution; NAD(P)H regeneration; Saturation mutagenesis; Thermostability

Indexed keywords

AMIDES; BIOCHEMISTRY; MOLECULAR MODELING; MUTAGENESIS; THERMODYNAMIC STABILITY;

DIRECTED EVOLUTION; NAD(P)H REGENERATION; NICOTINAMIDE; SATURATION MUTAGENESIS; THERMAL INACTIVATION; THERMOSTABILIZATION;

ENZYME ACTIVITY;

ENZYME VARIANT; OXIDOREDUCTASE; PHOSPHITE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; NAD PHOSPHITE OXIDOREDUCTASE; NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; UNCLASSIFIED DRUG;

ARTICLE; CHEMICAL ANALYSIS; ENZYME ACTIVITY; ENZYME INACTIVATION; ENZYME STABILITY; HALF LIFE TIME; MOLECULAR MODEL; MUTAGENESIS; MUTANT; MUTATION; SCREENING; DIRECTED MOLECULAR EVOLUTION; GENETICS; HEAT; METABOLISM; METHODOLOGY; STRUCTURE ACTIVITY RELATION;

DIRECTED MOLECULAR EVOLUTION; ENZYME STABILITY; HEAT; MUTAGENESIS; NADH, NADPH OXIDOREDUCTASES; NADP; STRUCTURE-ACTIVITY RELATIONSHIP;

EID: 38449096799     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.21546     Document Type: Article

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