Site-saturation mutagenesis: A powerful tool for structure-based design of combinatorial mutation libraries

Current Protocols in Protein Science

Volumn , Issue SUPPL.63, 2011, Pages

  Chronopoulou, Evangelia G ^a   Labrou, Nikolaos E ^a  

^a AGRICULTURAL UNIVERSITY OF ATHENS   (Greece)

Author keywords

Combinatorial mutation libraries; Mutagenesis; Protein engineering

Indexed keywords

OLIGONUCLEOTIDE; PLASMID DNA; PRIMER DNA; REAGENT; RESTRICTION ENDONUCLEASE; SINGLE STRANDED DNA;

AMINO ACID SUBSTITUTION; ARTICLE; BACTERIUM CULTURE; CLINICAL PROTOCOL; DNA DEGRADATION; DNA TEMPLATE; ESCHERICHIA COLI; GEL ELECTROPHORESIS; MUTATIONAL ANALYSIS; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN ENGINEERING; PROTEIN FUNCTION; PROTEIN VARIANT; SITE DIRECTED MUTAGENESIS; STRUCTURE ACTIVITY RELATION; BACTERIAL CELL; MUTAGENESIS; NONHUMAN; PLASMID; PRACTICE GUIDELINE; PROCESS DESIGN; SITE SATURATION MUTAGENESIS;

EID: 79953267172     PISSN: 19343655     EISSN: 19343663     Source Type: Journal    
DOI: 10.1002/0471140864.ps2606s63     Document Type: Article

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