Site-specific immobilization of recombinant antibody fragments through material-binding peptides for the sensitive detection of antigens in enzyme immunoassays

Biochimica et Biophysica Acta - Proteins and Proteomics

Volumn 1844, Issue 11, 2014, Pages 1960-1969

  Kumada, Yoichi ^a  

^a KYOTO INSTITUTE OF TECHNOLOGY   (Japan)

Author keywords

ELISA; Immobilization; Material binding peptide; Plastics

Indexed keywords

ANTIBODY; IMMUNOGLOBULIN F(AB) FRAGMENT; PLASTIC; POLY(METHYL METHACRYLATE); POLYSTYRENE; RECOMBINANT ANTIBODY;

ADSORPTION; ANTIBODY ENGINEERING; ANTIGEN BINDING; ARTICLE; BINDING AFFINITY; ENZYME IMMOBILIZATION; ENZYME LINKED IMMUNOSORBENT ASSAY; PROTEIN REFOLDING;

EID: 84907938891     PISSN: 15709639     EISSN: 18781454     Source Type: Journal    
DOI: 10.1016/j.bbapap.2014.07.007     Document Type: Review

References (108)

1. E. Engvall, and P. Perlmann Enzyme-linked immunosorbent assay (ELISA). Quantitative assay of immunoglobulin G Immunochemistry 8 1971 871 874
2. E. Engvall, and P. Perlmann Enzyme-linked immunosorbent assay, Elisa. 3. Quantitation of specific antibodies by enzyme-labeled anti-immunoglobulin in antigen-coated tubes J. Immunol. 109 1972 129 135
3. K. Itoh, and T. Suzuki Antibody-guided selection using capture-sandwich ELISA Methods Mol. Biol. 178 2002 195 199
4. B. Lu, M.R. Smyth, and R. O'Kennedy Oriented immobilization of antibodies and its applications in immunoassays and immunosensors Analyst 121 1996 29R 32R
5. A. Kondo, S. Oku, and K. Higashitani Adsorption of gamma-globulin, a model protein for antibody, on colloidal particles Biotechnol. Bioeng. 37 1991 537 543
6. M. Nisnevitch, and M.A. Firer The solid phase in affinity chromatography: strategies for antibody attachment J. Biochem. Biophys. Methods 49 2001 467 480
7. J.E. Butler Immunochemistry of Solid-Phase Immunoassay 1991 CRC Press Boca Raton, FL 3 26
8. J.E. Butler, P. Navarro, and J. Sun Adsorption-induced antigenic changes and their significance in ELISA and immunological disorders Immunol. Investig. 26 1997 39 54
9. J.E. Butler, L. Ni, R. Nessler, K.S. Joshi, M. Suter, B. Rosenberg, J. Chang, W.R. Brown, and L.A. Cantarero The physical and functional behavior of capture antibodies adsorbed on polystyrene J. Immunol. Methods 150 1992 77 90
10. X. Wang, Y. Wang, H. Xu, H. Shan, and J.R. Lu Dynamic adsorption of monoclonal antibody layers on hydrophilic silica surface: a combined study by spectroscopic ellipsometry and AFM J. Colloid Interface Sci. 323 2008 18 25
11. G.E. Kenny, and C.L. Dunsmoor Principles, problems, and strategies in the use of antigenic mixtures for the enzyme-linked immunosorbent assay J. Clin. Microbiol. 17 1983 655 665
12. H. Thakkar, C.L. Davey, E.A. Medcalf, L. Skingle, A.R. Craig, D.J. Newman, and C.P. Price Stabilization of turbidimetric immunoassay by covalent coupling of antibody to latex particles Clin. Chem. 37 1991 1248 1251
13. S.K. Vashist, C.K. Dixit, B.D. MacCraith, and R. O'Kennedy Effect of antibody immobilization strategies on the analytical performance of a surface plasmon resonance-based immunoassay Analyst 136 2011 4431 4436
14. J.A. Molina-Bolivar, F. Galisteo-Gonzalez, and R. Hidalgo-Alvarez Latex immunoassays: comparative studies on covalent and physical immobilization of antibodies. II. IgG J. Biomater. Sci. Polym. Ed. 9 1998 1103 1113
15. J.A. Molina-Bolivar, F. Galisteo-Gonzalez, and R. Hidalgo-Alvarez Latex immunoassays: comparative studies on covalent and physical immobilization of antibodies. I. F(ab′)2 fragments J. Biomater. Sci. Polym. Ed. 9 1998 1089 1101
16. S. Kanno, Y. Yanagida, T. Haruyama, E. Kobatake, and M. Aizawa Assembling of engineered IgG-binding protein on gold surface for highly oriented antibody immobilization J. Biotechnol. 76 2000 207 214
17. X. Xiao, X. Yang, T. Liu, Z. Chen, L. Chen, H. Li, and L. Deng Preparing a highly specific inert immunomolecular-magnetic beads for rapid detection and separation of S. aureus and group G Streptococcus Appl. Microbiol. Biotechnol. 75 2007 1209 1216
18. B. Madan, G. Chaudhary, S.M. Cramer, and W. Chen ELP-z and ELP-zz capturing scaffolds for the purification of immunoglobulins by affinity precipitation J. Biotechnol. 163 2013 10 16
19. Y. Jung, J.M. Lee, J.W. Kim, J. Yoon, H. Cho, and B.H. Chung Photoactivable antibody binding protein: site-selective and covalent coupling of antibody Anal. Chem. 81 2009 936 942
20. D.R. Buckler, A. Park, M. Viswanathan, R.M. Hoet, and R.C. Ladner Screening isolates from antibody phage-display libraries Drug Discov. Today 13 2008 318 324
21. H.R. Hoogenboom Overview of antibody phage-display technology and its applications Methods Mol. Biol. 178 2002 1 37
22. A. Pini, and L. Bracci Phage display of antibody fragments Curr. Protein Pept. Sci. 1 2000 155 169
23. D.L. Siegel Recombinant monoclonal antibody technology Transfus. Clin. Biol. 9 2002 15 22
24. R.J. Owens, and R.J. Young The genetic engineering of monoclonal antibodies J. Immunol. Methods 168 1994 149 165
25. E.S. Ward Antibody engineering using Escherichia coli as host Adv. Pharmacol. 24 1993 1 20
26. E. Dolk, M. van der Vaart, D. Lutje Hulsik, G. Vriend, H. de Haard, S. Spinelli, C. Cambillau, L. Frenken, and T. Verrips Isolation of llama antibody fragments for prevention of dandruff by phage display in shampoo Appl. Environ. Microbiol. 71 2005 442 450
27. E.B. Gottlin, G. Xiangrong, C. Pegram, A. Cannedy, M.J. Campa, and E.F. Patz Jr. Isolation of novel EGFR-specific VHH domains J. Biomol. Screen. 14 2009 77 85
28. D. Kastelic, S. Frkovic-Grazio, D. Baty, G. Truan, R. Komel, and D. Pompon A single-step procedure of recombinant library construction for the selection of efficiently produced llama VH binders directed against cancer markers J. Immunol. Methods 350 2009 54 62
29. R. Klooster, M.R. Eman, Q. le Duc, P. Verheesen, C.T. Verrips, R.C. Roovers, and J.A. Post Selection and characterization of KDEL-specific VHH antibody fragments and their application in the study of ER resident protein expression J. Immunol. Methods 342 2009 1 12
30. T. Katsuda, H. Sonoda, Y. Kumada, and H. Yamaji Production of antibody fragments in Escherichia coli Methods Mol. Biol. 907 2012 305 324
31. Y. Kumada, Y. Sakan, H. Kajihara, M. Kihara, Y. Kikuchi, H. Yamaji, G.H. Seong, and S. Katoh Efficient production of single-chain Fv antibody possessing rare codon linkers in fed-batch fermentation J. Biosci. Bioeng. 107 2009 73 77
32. H. Sonoda, Y. Kumada, T. Katsuda, and H. Yamaji Functional expression of single-chain Fv antibody in the cytoplasm of Escherichia coli by thioredoxin fusion and co-expression of molecular chaperones Protein Expr. Purif. 70 2010 248 253
33. S. Yamawaki, T. Matsumoto, Y. Ohnishi, Y. Kumada, N. Shiomi, T. Katsuda, E.K. Lee, and S. Katoh Production of single-chain variable fragment antibody (scFv) in fed-batch and continuous culture of Pichia pastoris by two different methanol feeding methods J. Biosci. Bioeng. 104 2007 403 407
34. M. Dragosits, G. Frascotti, L. Bernard-Granger, F. Vazquez, M. Giuliani, K. Baumann, E. Rodriguez-Carmona, J. Tokkanen, E. Parrilli, M.G. Wiebe, R. Kunert, M. Maurer, B. Gasser, M. Sauer, P. Branduardi, T. Pakula, M. Saloheimo, M. Penttila, P. Ferrer, M. Luisa Tutino, A. Villaverde, D. Porro, and D. Mattanovich Influence of growth temperature on the production of antibody Fab fragments in different microbes: a host comparative analysis Biotechnol. Prog. 27 2011 38 46
35. B. Gasser, M. Maurer, J. Gach, R. Kunert, and D. Mattanovich Engineering of Pichia pastoris for improved production of antibody fragments Biotechnol. Bioeng. 94 2006 353 361
36. M. Hust, T. Jostock, C. Menzel, B. Voedisch, A. Mohr, M. Brenneis, M.I. Kirsch, D. Meier, and S. Dubel Single chain Fab (scFab) fragment BMC Biotechnol. 7 2007 14
37. P. Martineau, P. Jones, and G. Winter Expression of an antibody fragment at high levels in the bacterial cytoplasm J. Mol. Biol. 280 1998 117 127
38. S. Miethe, T. Meyer, S. Wohl-Bruhn, A. Frenzel, T. Schirrmann, S. Dubel, and M. Hust Production of single chain fragment variable (scFv) antibodies in Escherichia coli using the LEX bioreactor J. Biotechnol. 163 2013 105 111
39. K.J. Mukherjee, D.C. Rowe, N.A. Watkins, and D.K. Summers Studies of single-chain antibody expression in quiescent Escherichia coli Appl. Environ. Microbiol. 70 2004 3005 3012
40. M. del Mar Garcia-Suarez, R. Villaverde, I. Gonzalez-Rodriguez, F. Vazquez, and F.J. Mendez Oriented immobilization of anti-pneumolysin tagged recombinant antibody fragments Curr. Microbiol. 59 2009 81 87
41. S. Howell, M. Kenmore, M. Kirkland, and R.A. Badley High-density immobilization of an antibody fragment to a carboxymethylated dextran-linked biosensor surface J. Mol. Recognit. 11 1998 200 203
42. I. Kurucz, J.A. Titus, C.R. Jost, and D.M. Segal Correct disulfide pairing and efficient refolding of detergent-solubilized single-chain Fv proteins from bacterial inclusion bodies Mol. Immunol. 32 1995 1443 1452
43. S.L. Seurynck-Servoss, C.L. Baird, K.D. Miller, N.B. Pefaur, R.M. Gonzalez, D.O. Apiyo, H.E. Engelmann, S. Srivastava, J. Kagan, K.D. Rodland, and R.C. Zangar Immobilization strategies for single-chain antibody microarrays Proteomics 8 2008 2199 2210
44. Z. Shen, H. Yan, Y. Zhang, R.L. Mernaugh, and X. Zeng Engineering peptide linkers for scFv immunosensors Anal. Chem. 80 2008 1910 1917
45. K. Yoshimoto, M. Nishio, H. Sugasawa, and Y. Nagasaki Direct observation of adsorption-induced inactivation of antibody fragments surrounded by mixed-PEG layer on a gold surface J. Am. Chem. Soc. 132 2010 7982 7989
46. K. Nakanishi, Y. Kumada, K. Imamura, and H. Imanaka Recent advances in controlled immobilization of proteins onto the surface of the solid substrate and its possible application to proteomics Curr. Proteomics 5 2008 161 175
47. M. Hedhammar, and S. Hober Protein engineering strategies for selective protein purification Chem. Eng. Technol. 28 2005 1315 1325
48. E. Hochuli Large-scale chromatography of recombinant proteins J. Chromatogr. 444 1988 293 302
49. J. Porath, J. Carlsson, I. Olsson, and G. Belfrage Metal chelate affinity chromatography, a new approach to protein fractionation Nature 258 1975 598 599
50. S. Chebil, I. Hafaiedh, H. Sauriat-Dorizon, N. Jaffrezic-Renault, A. Errachid, Z. Ali, and H. Korri-Youssoufi Electrochemical detection of D-dimer as deep vein thrombosis marker using single-chain D-dimer antibody immobilized on functionalized polypyrrole Biosens. Bioelectron. 26 2010 736 742
51. S.A. Parker, I.L. Diaz, K.A. Anderson, and C.A. Batt Design, production, and characterization of a single-chain variable fragment (ScFv) derived from the prostate specific membrane antigen (PSMA) monoclonal antibody J591 Protein Expr. Purif. 89 2013 136 145
52. S. Sommaruga, A. Lombardi, A. Salvade, S. Mazzucchelli, F. Corsi, P. Galeffi, P. Tortora, and D. Prosperi Highly efficient production of anti-HER2 scFv antibody variant for targeting breast cancer cells Appl. Microbiol. Biotechnol. 91 2011 613 621
53. J.Q. Guo, Q.M. Li, J.Y. Zhou, G.P. Zhang, Y.Y. Yang, G.X. Xing, D. Zhao, S.Y. You, and C.Y. Zhang Efficient recovery of the functional IP10-scFv fusion protein from inclusion bodies with an on-column refolding system Protein Expr. Purif. 45 2006 168 174
54. J.Q. Guo, S.Y. You, L. Li, Y.Z. Zhang, J.N. Huang, and C.Y. Zhang Construction and high-level expression of a single-chain Fv antibody fragment specific for acidic isoferritin in Escherichia coli J. Biotechnol. 102 2003 177 189
55. Y.S. Lo, D.H. Nam, H.M. So, H. Chang, J.J. Kim, Y.H. Kim, and J.O. Lee Oriented immobilization of antibody fragments on Ni-decorated single-walled carbon nanotube devices ACS Nano 3 2009 3649 3655
56. D.J. Manstein, H.P. Schuster, P. Morandini, and D.M. Hunt Cloning vectors for the production of proteins in Dictyostelium discoideum Gene 162 1995 129 134
57. T.G. Schmidt, and A. Skerra The random peptide library-assisted engineering of a C-terminal affinity peptide, useful for the detection and purification of a functional Ig Fv fragment Protein Eng. 6 1993 109 122
58. T. Maier, N. Drapal, M. Thanbichler, and A. Bock Strep-tag II affinity purification: an approach to study intermediates of metalloenzyme biosynthesis Anal. Biochem. 259 1998 68 73
59. N.M. Green Avidin Adv. Protein Chem. 29 1975 85 133
60. S. Ribrioux, G. Kleymann, W. Haase, K. Heitmann, C. Ostermeier, and H. Michel Use of nanogold- and fluorescent-labeled antibody Fv fragments in immunocytochemistry J. Histochem. Cytochem. 44 1996 207 213
61. D. Rau, K. Kramer, and B. Hock Cloning, functional expression and kinetic characterization of pesticide-selective Fab fragment variants derived by molecular evolution of variable antibody genes Anal. Bioanal. Chem. 372 2002 261 267
62. A. Skerra Bacterial expression of immunoglobulin fragments Curr. Opin. Immunol. 5 1993 256 262
63. R.J. Boado, A. Ji, and W.M. Pardridge Cloning and expression in Pichia pastoris of a genetically engineered single chain antibody against the rat transferrin receptor J. Drug Target. 8 2000 403 412
64. H. Cai, L. Chen, L. Wan, L. Zeng, H. Yang, S. Li, Y. Li, J. Cheng, and X. Lu High-level expression of a functional humanized anti-CTLA4 single-chain variable fragment antibody in Pichia pastoris Appl. Microbiol. Biotechnol. 82 2009 41 48
65. N. Carreras-Sangra, J. Tome-Amat, L. Garcia-Ortega, C.A. Batt, M. Onaderra, A. Martinez-del-Pozo, J.G. Gavilanes, and J. Lacadena Production and characterization of a colon cancer-specific immunotoxin based on the fungal ribotoxin alpha-sarcin Protein Eng. Des. Sel. 25 2012 425 435
66. A.E. Cunha, J.J. Clemente, R. Gomes, F. Pinto, M. Thomaz, S. Miranda, R. Pinto, D. Moosmayer, P. Donner, and M.J. Carrondo Methanol induction optimization for scFv antibody fragment production in Pichia pastoris Biotechnol. Bioeng. 86 2004 458 467
67. K. De Schutter, and N. Callewaert Pichia surface display: a tool for screening single domain antibodies Methods Mol. Biol. 911 2012 125 134
68. N.K. Khatri, D. Gocke, O. Trentmann, P. Neubauer, and F. Hoffmann Single-chain antibody fragment production in Pichia pastoris: benefits of prolonged pre-induction glycerol feeding Biotechnol. J. 6 2011 452 462
69. X. Shi, T. Karkut, M. Chamankhah, M. Alting-Mees, S.M. Hemmingsen, and D. Hegedus Optimal conditions for the expression of a single-chain antibody (scFv) gene in Pichia pastoris Protein Expr. Purif. 28 2003 321 330
70. B. Xu, J. Kriangkum, L.P. Nagata, R.E. Fulton, and M.R. Suresh A single chain Fv specific against Western equine encephalitis virus Hybridoma 18 1999 315 323
71. M. Sarikaya, C. Tamerler, A.K. Jen, Y.K. Schulten, and F. Baneyx Molecular biomimetics: nanotechnology through biology Nat. Mater. 2 2003 577 585
72. K. Hashimoto, M. Yoshinari, K. Matsuzaka, K. Shiba, and T. Inoue Identification of peptide motif that binds to the surface of zirconia Dent. Mater. J. 30 2011 935 940
73. D. Kase, J.L. Kulp III, M. Yudasaka, J.S. Evans, S. Iijima, and K. Shiba Affinity selection of peptide phage libraries against single-wall carbon nanohorns identifies a peptide aptamer with conformational variability Langmuir 20 2004 8939 8941
74. K. Sano, H. Sasaki, and K. Shiba Specificity and biomineralization activities of Ti-binding peptide-1 (TBP-1) Langmuir 21 2005 3090 3095
75. K. Sano, and K. Shiba A hexapeptide motif that electrostatically binds to the surface of titanium J. Am. Chem. Soc. 125 2003 14234 14235
76. T. Ikeda, and A. Kuroda Why does the silica-binding protein "Si-tag" bind strongly to silica surfaces? Implications of conformational adaptation of the intrinsically disordered polypeptide to solid surfaces Colloids Surf. B: Biointerfaces 86 2011 359 363
77. T. Ikeda, K. Motomura, Y. Agou, T. Ishida, R. Hirota, and A. Kuroda The silica-binding Si-tag functions as an affinity tag even under denaturing conditions Protein Expr. Purif. 77 2011 173 177
78. T. Ikeda, K. Ninomiya, R. Hirota, and A. Kuroda Single-step affinity purification of recombinant proteins using the silica-binding Si-tag as a fusion partner Protein Expr. Purif. 71 2010 91 95
79. T. Ikeda, Y. Hata, K. Ninomiya, Y. Ikura, K. Takeguchi, S. Aoyagi, R. Hirota, and A. Kuroda Oriented immobilization of antibodies on a silicon wafer using Si-tagged protein A Anal. Biochem. 385 2009 132 137
80. K. Taniguchi, K. Nomura, Y. Hata, T. Nishimura, Y. Asami, and A. Kuroda The Si-tag for immobilizing proteins on a silica surface Biotechnol. Bioeng. 96 2007 1023 1029
81. H. Matsuno, J. Sekine, H. Yajima, and T. Serizawa Biological selection of peptides for poly(l-lactide) substrates Langmuir 24 2008 6399 6403
82. T. Serizawa, T. Sawada, and H. Matsuno Highly specific affinities of short peptides against synthetic polymers Langmuir 23 2007 11127 11133
83. T. Serizawa, T. Sawada, H. Matsuno, T. Matsubara, and T. Sato A peptide motif recognizing a polymer stereoregularity J. Am. Chem. Soc. 127 2005 13780 13781
84. B. Feng, Y. Dai, L. Wang, N. Tao, S. Huang, and H. Zeng A novel affinity ligand for polystyrene surface from a phage display random library and its application in anti-HIV-1 ELISA system Biologicals 37 2009 48 54
85. Y. Kumada, Y. Tokunaga, H. Imanaka, K. Imamura, T. Sakiyama, S. Katoh, and K. Nakanishi Screening and characterization of affinity peptide tags specific to polystyrene supports for the orientated immobilization of proteins Biotechnol. Prog. 22 2006 401 405
86. Y. Kumada, Y. Shiritani, K. Hamasaki, T. Ohse, and M. Kishimoto High biological activity of a recombinant protein immobilized onto polystyrene Biotechnol. J. 4 2009 1178 1189
87. Y. Kumada, D. Kuroki, H. Yasui, T. Ohse, and M. Kishimoto Characterization of polystyrene-binding peptides (PS-tags) for site-specific immobilization of proteins J. Biosci. Bioeng. 109 2010 583 587
88. Y. Kumada, S. Murata, Y. Ishikawa, K. Nakatsuka, and M. Kishimoto Screening of PC and PMMA-binding peptides for site-specific immobilization of proteins J. Biotechnol. 160 2012 222 228
89. Y. Kumada, T. Ootsuka, M. Asada, S. Yoshizuka, M. Chiyama, M. Sakane, H.M. Fida, K. Sawada, K. Okumura, and M. Kishimoto Identification and characterization of peptide fragments for the direct and site-specific immobilization of functional proteins onto the surface of silicon nitride J. Biotechnol. 184C 2014 103 110
90. J.M. Kogot, D.A. Sarkes, I. Val-Addo, P.M. Pellegrino, and D.N. Stratis-Cullum Increased affinity and solubility of peptides used for direct peptide ELISA on polystyrene surfaces through fusion with a polystyrene-binding peptide tag Biotechniques 52 2012 95 102
91. S. Konagaya, K. Kato, T. Nakaji-Hirabayashi, and H. Iwata Design of culture substrates for large-scale expansion of neural stem cells Biomaterials 32 2011 992 1001
92. J.B. Tang, X.F. Sun, H.M. Yang, B.G. Zhang, Z.J. Li, Z.J. Lin, and Z.Q. Gao Well-oriented ZZ-PS-tag with high Fc-binding onto polystyrene surface for controlled immobilization of capture antibodies Anal. Chim. Acta 776 2013 74 78
93. Y. Kumada, S. Katoh, H. Imanaka, K. Imamura, and K. Nakanishi Development of a one-step ELISA method using an affinity peptide tag specific to a hydrophilic polystyrene surface J. Biotechnol. 127 2007 288 299
94. S.Y. Hwang, Y. Kumada, G.H. Seong, J. Choo, S. Katoh, and E.K. Lee Characteristics of a liposome immunoassay on a poly(methyl methacrylate) surface Anal. Bioanal. Chem. 389 2007 2251 2257
95. Y. Kumada, K. Hamasaki, Y. Shiritani, T. Ohse, and M. Kishimoto Efficient immobilization of a ligand antibody with high antigen-binding activity by use of a polystyrene-binding peptide and an intelligent microtiter plate J. Biotechnol. 142 2009 135 141
96. Y. Kumada, C. Zhao, R. Ishimura, H. Imanaka, K. Imamura, and K. Nakanishi Protein-protein interaction analysis using an affinity peptide tag and hydrophilic polystyrene plate J. Biotechnol. 128 2007 354 361
97. T. Furuta, T. Ogawa, T. Katsuda, I. Fujii, and H. Yamaji Efficient production of an antibody Fab fragment using the baculovirus-insect cell system J. Biosci. Bioeng. 110 2010 577 581
98. T. Furuta, T. Ogawa, and H. Yamaji Production of antibody fragments using the baculovirus-insect cell system Methods Mol. Biol. 907 2012 371 387
99. C. Lemeulle, T. Chardes, C. Montavon, H. Chaabihi, J.C. Mani, M. Pugniere, M. Cerutti, G. Devauchelle, B. Pau, and M. Biard-Piechaczyk Anti-digoxin scFv fragments expressed in bacteria and in insect cells have different antigen binding properties FEBS Lett. 423 1998 159 166
100. M. Liang, S. Dubel, D. Li, I. Queitsch, W. Li, and E.K. Bautz Baculovirus expression cassette vectors for rapid production of complete human IgG from phage display selected antibody fragments J. Immunol. Methods 247 2001 119 130
101. U. Fiedler, J. Phillips, O. Artsaenko, and U. Conrad Optimization of scFv antibody production in transgenic plants Immunotechnology 3 1997 205 216
102. F. Shahryari, M.R. Safarnejad, M. Shams-Bakhsh, S. Schillberg, and G. Nolke Generation and expression in plants of a single-chain variable fragment antibody against the immunodominant membrane protein of Candidatus phytoplasma aurantifolia J. Microbiol. Biotechnol. 23 2013 1047 1054
103. G. Sunilkumar, S.D. Waghela, L.M. Campbell, and K.S. Rathore Expression of anti-K99 scFv in transgenic rice tissues and its functional characterization Transgenic Res. 18 2009 347 360
104. B. Van Droogenbroeck, K. De Wilde, and A. Depicker Production of antibody fragments in Arabidopsis seeds Methods Mol. Biol. 483 2009 89 101
105. Y. Kumada, K. Hamasaki, Y. Shiritani, A. Nakagawa, D. Kuroki, T. Ohse, D.H. Choi, Y. Katakura, and M. Kishimoto Direct immobilization of functional single-chain variable fragment antibodies (scFvs) onto a polystyrene plate by genetic fusion of a polystyrene-binding peptide (PS-tag) Anal. Bioanal. Chem. 395 2009 759 765
106. Y. Kumada, Y. Ohigashi, Y. Emori, K. Imamura, Y. Omura, and M. Kishimoto Improved lectin ELISA for glycosylation analysis of biomarkers using PS-tag-fused single-chain Fv J. Immunol. Methods 385 2012 15 22
107. Y. Kumada, K. Hamasaki, A. Nakagawa, E. Sasaki, T. Shirai, M. Okumura, M. Inoue, and M. Kishimoto Immobilization and functional reconstitution of antibody Fab fragment by solid-phase refolding J. Immunol. Methods 400-401 2013 70 77
108. Y. Kumada, Y. Ishikawa, Y. Fujiwara, R. Takeda, R. Miyamoto, D. Niwa, S. Momose, B. Kang, and M. Kishimoto Efficient refolding and immobilization of PMMA-tag-fused single-chain Fv antibodies for sensitive immunological detection on a PMMA plate J. Immunol. Methods 2014 10.1016/j.jim.2014.05.015 (in press)