Use of nanogold- and fluorescent-labeled antibody Fv fragments in immunocytochemistry

Journal of Histochemistry and Cytochemistry

Volumn 44, Issue 3, 1996, Pages 207-213

  Ribrioux, Sebastien ^a   Kleymann, Gerald ^a,b   Haase, Winfried ^a   Heitmann, Karin ^a   Ostermeier, Christian ^a   Michel, Hartmut ^a  

^a MAX PLANCK INSTITUTE OF BIOPHYSICS   (Germany)

^b BAYER AG   (Germany)

Author keywords

Bacteriorhodopsin; Cytochrome c oxidase; Electron microscopy; Engineered antibody Fv fragments; Fluorescence labeling; Halobacterium halobium; Membrane protein; Nanogold labeling; Paracoccus denitrificans; Ubiquinol:cytochrome c oxidoreductase

Indexed keywords

ANTIBODY; BACTERIORHODOPSIN; CYTOCHROME C OXIDASE; EPITOPE; FLUORESCEIN; GOLD DERIVATIVE; IMMUNOGLOBULIN F(AB) FRAGMENT; MEMBRANE PROTEIN;

AFFINITY CHROMATOGRAPHY; ARTICLE; CONTROLLED STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; FLUORESCENCE; HALOBACTERIUM SALINARUM; IMMUNOCYTOCHEMISTRY; IMMUNOELECTRON MICROSCOPY; MICROSCOPY; NONHUMAN; PARACOCCUS DENITRIFICANS; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL;

EID: 0029927523     PISSN: 00221554     EISSN: None     Source Type: Journal    
DOI: 10.1177/44.3.8648079     Document Type: Article

References (31)

1. Padlan EA Anatomy of the antibody molecule. Mol Immunol 1994;19.169
2. Köhler G, Milstein C. Continuous cultures of fused cells secreting antibody of predefined specificity. Nature 1975;256:495
3. Skerra A, Plückthun, A. Assembly of a functional immunoglobulin Fv fragment in Escherichia coli. Science 1988;240:1038
4. Bird RE, Hardmann KD, Jacobson JW, Johnson S, Kaufman BM, Lee S, Lee T, Pope SH, Riordan GS, Whitlow M. Single-chain antigenbinding proteins. Science 1988;242:423
5. Better M, Chang CP, Robinson RR, Horwitz AH. Escherichia coli secretion of an active chimeric antibody fragment. Science 1988;240:1041.
6. Poljak RJ. Production and structure of diabodies. Structure 1994;2:1121
7. Winter G, Milstein C. Man-made antibodies. Nature 1991;349:293
8. Winter G, Griffiths AD, Hawkins RE, Hoogenboom HR. Making antibodies by phage display technology. Annu Rev Immunol 1994;12:433
9. Reiter Y, Brinkmann U, Jung SH, Lee B, Kasprzyk PG, King CR, Pastan I Improved binding and antitumor activity of a recombinant anti-erbB2 immunotoxin by disulfide stabilization of the Fv fragment. J Biol Chem 1994;269:18327
10. Kleymann G, Ostermeier C, Ludwig B, Skerra A, Michel H. Engineered Fv fragments as a tool for the one-step purification of integral multisubumt membrane protein complexes. BioTechnology 1995;13:155
11. Iwata S, Ostermeier C, Ludwig B, Michel H. Structure at 2.8 Å resolution of cytochrome c oxidase from Paracoccus denitrificans. Nature 1995;376:660
12. Kleymann G, Ostermeier C, Heitmann K, Haase W, Michel H. Use of antibody fragments (Fv) in immunocytochemistry. J Histochem Cytochem 1995;43:607
13. Yamsh-Peron C, Vicra J, Messing J. Improved M13 phage cloning vectors and host strains, nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 1985;33:103
14. Schmidt TGM, Skerra A. One-step affinity purification of bacterially produced proteins by means of the Strep tag and immobilized recombinant core streptavidin. J Chromatogr 1994;676:337
15. Gill SC, Hippel PH. Calculation of protein extinction coefficients from amino acid sequence data. Anal Biochem 1989;182:319
16. Spurr AR. A low-viscosity epoxy resin embedding medium for electron microscopy J Ultrastruct Res 1969;26 31
17. Malmqvist M. Surface plasmon resonance for the detection and measurement of antibody-antigen affinity and kinetics. Curr Opin Immunol 1993;5:282
18. Coons AH, Creech HJ, Jones RN. Immunological properties of an antibody containing a fluorescent group Proc Soc Exp Biol 1941;47:200
19. Singer SJ Preparation of an electron dense antibody conjugate. Nature 1959;183:1523
20. Feldherr CM, Marshall JM. The use of colloidal gold for studies of intracellular exchange in amoeba Chaos chaos. J Cell Biol 1962;12:640
21. Faulk WP, Taylor GM An immunocolloid method for the electron microscope Immunochemistry 1971;8 1081
22. Hainfeld JF. A small gold-conjugated antibody label: improved resolution for electron microscopy. Science 1987;236:450
23. Hainfeld JF, Furuya FR. A 1.4-nm gold cluster covalently attached to antibodies improves immunolabeling J Histochem Cytochem 1992; 40:177
24. Griffiths G. Fine structure immunocytochemistry. Berlin, Heidelberg: Springer-Verlag, 1993
25. Kleymann G, Iwata S, Wiesmüller KH, Ludwig B, Haase W, Michel H. Immunoelectron microscopy and epitope mapping with monoclonal antibodies suggest the existence of an additional N-terminal transmembrane helix in the cytochrome b subunit of bacterial ubiquinol: cytochrome-c oxidoreductases. Eur J Biochem 1995;230:359
26. Wong SS Chemistry of protein conjugation and crosslinking. Boca Raton, FL: CRC Press, 1991
27. 7 Skerra A. Bacterial expression of immunoglobulin fragments. Curr Opin Immunol 1993;5:256
28. Dorai H, McCartney JE, Hudziak RM, Tai MS, Larainet AA, Houston LL, Huston JS, Oppermann H. Mammalian cell expression of singlechain Fv (sFv) antibody proteins and their C-terminal fusion with interleukin-2 and other effector domains. BioTechnology 1994;12:890
29. Jost CR, Kurucz I, Jacobus CM, Titus JA, George AJT, Segal DM. Mammalian expression and secretion of functional single-chain Fv molecules. J Biol Chem 1994;269.26267
30. Kabat EA, Wu TT, Perry HM, Gottesman KS, Foeller C. Sequences of proteins of immunological interest. 5th ed. Bethesda, MD: US Department of Health and Human Services, US Government Printing Office, 1991
31. Tschopp J, Podack ER, Müller-Eberhard HJ. Utuastructure of the membrane attack complex of complement: detection of the tetramolecular C9-polymerizing complex C5b-8. Proc Natl Acad Sci USA 1982;79:7474