Direct immobilization of functional single-chain variable fragment antibodies (scFvs) onto a polystyrene plate by genetic fusion of a polystyrene-binding peptide (PS-tag)

Analytical and Bioanalytical Chemistry

Volumn 395, Issue 3, 2009, Pages 759-765

  Kumada, Yoichi ^a   Hamasaki, Kyoko ^a   Shiritani, Yuki ^a   Nakagawa, Aya ^a   Kuroki, Daisuke ^a   Ohse, Takuhito ^b   Choi, Dong Hwan ^c   Katakura, Yoshio ^d   Kishimoto, Michimasa ^a  

^a KYOTO INSTITUTE OF TECHNOLOGY   (Japan)

^b Enplas Corporation   (Japan)

^c Korea Research Institute of Bioscience and Biotechnology (KRIBB)   (South Korea)

^d OSAKA UNIVERSITY   (Japan)

Author keywords

One step ELISA; Orientation control; Polystyrene; Polystyrene binding peptide (PS tag); Single chain fragment of variable domain (scFv); Site specific immobilization

Indexed keywords

ANTIGEN-BINDING ACTIVITIES; BINDING PEPTIDE; C-REACTIVE PROTEINS; CLINICAL DIAGNOSIS; CONFORMATIONAL CHANGE; DILUTION METHOD; ENZYME LINKED IMMUNOSORBENT ASSAY; FUNCTIONALIZED; INCLUSION BODIES; MONOCLONAL ANTIBODIES (MAB); ONE-STEP ELISA; ORIENTATION CONTROL; POLYSTYRENE SUPPORT; RECOMBINANT ESCHERICHIA COLI; SINGLE-CHAIN FRAGMENT OF VARIABLE DOMAIN (SCFV); SINGLE-CHAIN FV; SITE-SPECIFIC; SITE-SPECIFIC IMMOBILIZATION; TWEEN-20; VARIABLE FRAGMENT;

AMINES; ANTIGENS; DIAGNOSIS; ESCHERICHIA COLI; LIGANDS; MONOCLONAL ANTIBODIES; PEPTIDES; POLYSTYRENES;

BINDING SITES;

C REACTIVE PROTEIN; HYBRID PROTEIN; IMMOBILIZED ANTIBODY; PEPTIDE; POLYSTYRENE DERIVATIVE;

AMINO ACID SEQUENCE; ANTIBODY AFFINITY; ARTICLE; CHEMISTRY; ECONOMICS; ENZYME LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; GENETICS; HUMAN; IMMUNOGLOBULIN VARIABLE REGION; IMMUNOLOGY; METHODOLOGY; PROTEIN STABILITY; TIME;

AMINO ACID SEQUENCE; ANTIBODIES, IMMOBILIZED; ANTIBODY AFFINITY; C-REACTIVE PROTEIN; ENZYME-LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; HUMANS; IMMUNOGLOBULIN VARIABLE REGION; PEPTIDES; POLYSTYRENES; PROTEIN STABILITY; RECOMBINANT FUSION PROTEINS; TIME FACTORS;

ESCHERICHIA COLI;

EID: 70349615664     PISSN: 16182642     EISSN: 16182650     Source Type: Journal    
DOI: 10.1007/s00216-009-2999-y     Document Type: Article

References (15)

1. E Engvall K Jonsson P Perlmann 1971 Biochim Biophys Acta 251 427 434
2. E Engvall P Perlmann 1972 J Immunol 109 129 135
3. K Nakanishi T Sakiyama Y Kumada K Imamura H Imanaka 2008 Current Proteomics 5 161 175
4. Y Jung JY Jeong BH Chung 2008 Analyst 133 697 701
5. AA Kortt RL Malby JB Caldwell LC Gruen NM Ivancic MC Lawrence GJ Howlett RG Webster PJ Hudson PM Calman 1994 Eur J Biochem 221 151 157
6. HR Hoogenboom AP De Bruïne SE Hufton RM Hoet J-W Arends RC Roovers 1998 Immunotechnology 4 1 20
7. C Gao S Mao G Kaufmann P Wirsching RA Lerner KD Janda 2002 Proc Natl Acad Sci U S A 99 12612 12616
8. Y Kumada Y Tokunaga H Imanaka K Imamura T Sakiyama S Katoh K Nakanishi 2006 Biotechnol Prog 22 401 405
9. Kumada Y, Shiritani Y, Hamasaki K, Ohse T, Kisihmoto M. (2009) Biotechnol J, in press
10. Y Kumada K Hamasaki Y Shiritani T Ohse M Kisihmoto 2009 J Biotechnol 142 135 141
11. Y Kumada S Katoh H Imanaka K Imamura K Nakanishi 2007 J Biotechnol 127 288 299
12. Y Kumada C Zhao R Ishimura H Imanaka K Imamura K Nakanishi 2007 J Biotechnol 128 354 361
13. S Katoh M Terashima 1994 Appl Microbiol Biotechnol 42 36 39
14. DH Choi Y Katakura K Ninomiya S Shioya 2008 J Biosci Bioeng 105 261 272
15. SY Hwang Y Kumada GH Seong JB Choo S Katoh EK Lee 2008 Anal Bioanal Chem 389 2251 2257