Characterization of polystyrene-binding peptides (PS-tags) for site-specific immobilization of proteins

Journal of Bioscience and Bioengineering

Volumn 109, Issue 6, 2010, Pages 583-587

  Kumada, Yoichi ^a   Kuroki, Daisuke ^a   Yasui, Hidefumi ^a   Ohse, Takuhito ^b   Kishimoto, Michimasa ^a  

^a KYOTO INSTITUTE OF TECHNOLOGY   (Japan)

^b Enplas Corporation   (Japan)

Author keywords

Affinity peptide; Glutathione S transferase (GST); Polystyrene; PS tag; Site specific protein immobilization

Indexed keywords

AFFINITY PEPTIDES; GLUTATHIONE S-TRANSFERASE (GST); GLUTATHIONE-S-TRANSFERASE; PROTEIN IMMOBILIZATION; SITE-SPECIFIC;

ADSORPTION; AMINO ACIDS; BINDING ENERGY; BIOASSAY; BIOCHIPS; DISSOCIATION; HYDROPHILICITY; ORGANIC ACIDS; POLYMER BLENDS; POLYSTYRENES; SODIUM CHLORIDE; SURFACE ACTIVE AGENTS; UREA;

PEPTIDES;

ARGINYLALANYLPHENYLALANYLISOLEUCYLALANYLSERYLARGINYLARGINYLISOLEUCYLLYSYLARGINYLPROLINE; ARGINYLISOLEUCYLISOLEUCYLISOLEUCYLARGINYLARGINYLISOLEUCYLARGINYLARGININE; GLUTATHIONE TRANSFERASE; ISOLEUCINE; LEUCINE; PEPTIDE; POLYSORBATE 20; POLYSTYRENE; SODIUM CHLORIDE; UNCLASSIFIED DRUG; UREA;

ADSORPTION; ARTICLE; BINDING AFFINITY; DISSOCIATION CONSTANT; ENZYME ACTIVITY; HYDROPHILICITY; HYDROPHOBICITY; NONHUMAN; PROTEIN IMMOBILIZATION; QUANTITATIVE ANALYSIS;

ADSORPTION; AMINO ACIDS; FLUORESCEIN-5-ISOTHIOCYANATE; GLUTATHIONE TRANSFERASE; HYDROPHOBICITY; IMMOBILIZED PROTEINS; LEUCINE; PEPTIDES; POLYSTYRENES; RECOMBINANT FUSION PROTEINS;

EID: 77952101293     PISSN: 13891723     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiosc.2009.11.005     Document Type: Article

References (20)

1. MacBeath G. Protein microarrays and proteomics. Nat. Genet. 32 (2002) 526-532
2. Zhu H., and Snyder M. Protein chip technology. Curr. Opin. Chem. Biol. 7 (2003) 55-63
3. Murakami Y., Endo T., Yamamura S., Nagatani N., Takamura Y., and Tamiya E. On-chip micro-flow polystyrene bead-based immunoassay for quantitative detection of tacrolimus (FK506). Anal. Biochem. 334 (2004) 111-116
4. Zhang K., Diehl M.R., and Tirrell D.A. Artificial polypeptide scaffold for protein immobilization. J. Am. Chem. Soc. 127 (2005) 10136-10137
5. Butterfield D.A., Bhattacharyya D., Daunert S., and Bachas L. Catalytic biofunctional membranes containing site-specifically immobilized enzyme arrays: A review. J. Membr. Sci. 181 (2001) 29-37
6. Lutz H., Stammler P., and Fischer E.A. Covalent binding of detergent-solubilized membrane glycoproteins to 'Chemobond' plates for ELISA. J. Immunol. Methods 129 (1990) 211-220
7. Nakanishi K., Sakiyama T., Kumada Y., Imamura K., and Imanaka H. Recent advances in controlled immobilization of proteins onto the surface of the solid substrate and its possible application to proteomics. Current Proteomics 5 (2008) 161-175
8. Hwang S.Y., Kumada Y., Seong G.H., Choo J.B., Katoh S., and Lee E.K. Characteristics of a liposome immunoassay on a poly (methyl methacrylate) surface. Anal. Bioanal. Chem. 389 (2007) 2251-2257
9. Prickett K.S., Amberg D.C., and Hopp T.P. Calcium-dependent antibody for identification and purification of recombinant proteins. Biotechniques 7 (1989) 580-589
10. Porath J., Carlsson J., Olsson I., and Belfrage G. Metal chelate affinity chromatography, a new approach to protein fractionation. Nature 258 (1975) 598-599
11. Schmidt T.G., and Skerra A. The random peptide library-assisted engineering of a C-terminal affinity peptide, useful for the detection and purification of a functional Ig Fv fragment. Protein Eng. 6 (1993) 109-122
12. Hedhammar M., Graslund T., and Hober S. Protein engineering strategies for selective protein purification. Chem. Eng. Technol. 28 (2005) 1315-1325
13. Kumada Y., Tokunaga Y., Imanaka H., Imamura K., Sakiyama T., Katoh S., and Nakanishi K. Screening and characterization of affinity peptide tags specific to polystyrene supports for the orientated immobilization of proteins. Biotechnol. Prog. 22 (2006) 401-405
14. Kumada Y., Katoh S., Imanaka H., Imamura K., and Nakanishi K. Development of a one-step ELISA method using an affinity peptide tag specific to a hydrophilic polystyrene surface. J. Biotechnol. 127 (2007) 288-299
15. Kumada Y., Zhao C., Ishimura R., Imanaka H., Imamura K., and Nakanishi K. Protein-protein interaction analysis using an affinity peptide tag and hydrophilic polystyrene plate. J. Biotechnol. 128 (2007) 354-361
16. Kumada Y., Shiritani Y., Hamasaki K., Ohse T., and Kisihmoto M. High biological activity of a recombinant protein immobilized onto polystyrene. Biotech. J. 4 (2009) 1190-1197
17. Kumada Y., Hamasaki K., Shiritani Y., Ohse T., and Kisihmoto M. Efficient immobilization of a ligand antibody with high antigen-binding activity by use of a polystyrene binding peptide and an intelligent microtiter plate. J. Biotechnol. 142 (2009) 135-141
18. Kumada Y., Hamasaki K., Shiritani Y., Nakagawa A., Kuroki D., Ohse T., Choi D.H., Katakura Y., and Kishimoto M. Direct immobilization of functional single-chain variable fragment antibodies (scFvs) onto a polystyrene plate by genetic fusion of a polystyrene-binding peptide (PS-tag). Anal. Bioanal. Chem. 395 (2009) 759-765
19. Katoh S., and Terashima M. Purification of secreted α-amylases by immunoaffinity chromatography with cross-reactive antibody. Appl. Microbiol. Biotechnol. 42 (1994) 36-39
20. Milano ion-exchange adsorption: Equilibrium uptake behavior and protein mass transfer kinetics. Biotech. J. 2 (2007) 110-120