High-level expression of a functional humanized anti-CTLA4 single-chain variable fragment antibody in Pichia pastoris

Applied Microbiology and Biotechnology

Volumn 82, Issue 1, 2009, Pages 41-48

  Cai, Huawei ^a   Chen, Lihong ^a,b   Wan, Lin ^a   Zeng, Lingyu ^c   Yang, Hao ^a   Li, Shengfu ^a   Li, Youping ^a   Cheng, Jingqiu ^a   Lu, Xiaofeng ^a  

^a SICHUAN UNIVERSITY   (China)

^b HONG KONG UNIVERSITY OF SCIENCE AND TECHNOLOGY   (Hong Kong)

^c XUZHOU MEDICAL UNIVERSITY   (China)

Author keywords

CTLA 4; Immunotherapy; Pichia pastoris; Single chain variable fragment antibody

Indexed keywords

BINDING ABILITIES; CTLA-4; CYTOTOXIC T LYMPHOCYTE (CTL); EXPRESSION SYSTEM; EXPRESSION VECTORS; HIGH LEVEL EXPRESSION; HIS TAG; IMMUNE RESPONSES; IMMUNOTHERAPY; INDUCTION TIME; METHANOL CONCENTRATIONS; P. PASTORIS; PICHIA PASTORIS; RAJI CELLS; SINGLE-CHAIN VARIABLE FRAGMENT ANTIBODY; TRANSPLANT REJECTION; VARIABLE FRAGMENT;

CELL CULTURE; GENE ENCODING; METHANOL;

ANTIBODIES;

ANTIBODY; GENE EXPRESSION; TOXIN; YEAST;

ANTIBODIES; ANTIGENS, CD; CELL LINE; CLONING, MOLECULAR; GENE EXPRESSION; GENETIC VECTORS; HUMANS; IMMUNOGLOBULIN VARIABLE REGION; IMMUNOTOXINS; PICHIA; PROTEIN ENGINEERING;

PICHIA PASTORIS;

EID: 58549105076     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-008-1744-3     Document Type: Article

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