Production of single chain fragment variable (scFv) antibodies in Escherichia coli using the LEX™ bioreactor

Journal of Biotechnology

Volumn 163, Issue 2, 2013, Pages 105-111

  Miethe, Sebastian ^a   Meyer, Torsten ^b   Wöhl Bruhn, Stefanie ^a   Frenzel, André ^a   Schirrmann, Thomas ^a   Dübel, Stefan ^a   Hust, Michael ^a  

^a TECHNICAL UNIVERSITY OF BRAUNSCHWEIG   (Germany)

^b MAb factory GmbH   (Germany)

Author keywords

Antibody; Antibody fragments; Escherichia coli; LEX bioreactor; Production; Proteome; ScFv; Single chain fragment variable

Indexed keywords

ANTIBODY FRAGMENT; BACTERIAL PRODUCTION; HIGH THROUGHPUT; IN-VITRO; OPTIMIZED PROTOCOL; PRODUCTION PARAMETERS; SCFV; SHAKING FLASKS; SINGLE CHAINS;

BIOCONVERSION; BIOREACTORS; ESCHERICHIA COLI; PRODUCTION; PROTEINS; THROUGHPUT;

ANTIBODIES;

SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY;

AFFINITY CHROMATOGRAPHY; ARTICLE; BIOTECHNOLOGICAL PRODUCTION; BUBBLE COLUMN REACTOR; CONTROLLED STUDY; ESCHERICHIA COLI; EXPRESSION VECTOR; GENOTYPE; INTERMETHOD COMPARISON; LARGE SCALE PRODUCTION; NONHUMAN; PRIORITY JOURNAL; REACTOR OPTIMIZATION;

BIOREACTORS; BIOTECHNOLOGY; ENZYME-LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; RECOMBINANT FUSION PROTEINS; SINGLE-CHAIN ANTIBODIES;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 84872177505     PISSN: 01681656     EISSN: 18734863     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2012.07.011     Document Type: Article

References (37)

1. Bardwell J.C., Lee J.O., Jander G., Martin N., Belin D., Beckwith J. A pathway for disulfide bond formation in vivo. Proceedings of the National Academy of Sciences of the United States of America 1993, 90:1038-1042.
2. Boel E., Verlaan S., Poppelier M.J., Westerdaal N.A., Van Strijp J.A., Logtenberg T. Functional human monoclonal antibodies of all isotypes constructed from phage display library-derived single-chain Fv antibody fragments. Journal of Immunological Methods 2000, 239:153-166.
3. Breitling F., Dübel S., Seehaus T., Klewinghaus I., Little M. A surface expression vector for antibody screening. Gene 1991, 104:147-153.
4. Buckler D.R., Park A., Viswanathan M., Hoet R.M., Ladner R.C. Screening isolates from antibody phage-display libraries. Drug Discovery Today 2008, 13:318-324.
5. Chahboun S., Hust M., Liu Y., Pelat T., Miethe S., Helmsing S., Jones R.G., Sesardic D., Thullier P. Isolation of a nanomolar scFv inhibiting the endopeptidase activity of botulinum toxin A, by single-round panning of an immune phage-displayed library of macaque origin. BMC Biotechnology 2011, 11:113.
6. Colwill K., Gräslund S. A roadmap to generate renewable protein binders to the human proteome. Nature Methods 2011, 8:551-558.
7. Dübel S., Breitling F., Klewinghaus I., Little M. Regulated secretion and purification of recombinant antibodies in E. coli. Cell Biophysics 1992, 21:69-79.
8. Dübel S., Stoevesandt O., Taussig M.J., Hust M. Generating recombinant antibodies to the complete human proteome. Trends in Biotechnology 2010, 28:333-339.
9. Goletz S., Christensen P.A., Kristensen P., Blohm D., Tomlinson I., Winter G., Karsten U. Selection of large diversities of antiidiotypic antibody fragments by phage display. Journal of Molecular Biology 2002, 315:1087-1097.
10. Gottesman S., Wickner S., Maurizi M.R. Protein quality control: triage by chaperones and proteases. Genes and Development 1997, 11:815-823.
11. Gräslund S., Sagemark J., Berglund H., Dahlgren L.-G., Flores A., Hammarström M., Johansson I., Kotenyova T., Nilsson M., Nordlund P., Weigelt J. The use of systematic N- and C-terminal deletions to promote production and structural studies of recombinant proteins. Protein Expression and Purification 2008, 58:210-221.
12. Hallborn J., Carlsson R. Automated screening procedure for high-throughput generation of antibody fragments. BioTechniques 2002, Suppl:30-37.
13. Hoogenboom H.R., Griffiths A.D., Johnson K.S., Chiswell D.J., Hudson P., Winter G. Multi-subunit proteins on the surface of filamentous phage: methodologies for displaying antibody (Fab) heavy and light chains. Nucleic Acids Research 1991, 19:4133-4137.
14. Hust M., Dübel S., Schirrmann T. Selection of recombinant antibodies from antibody gene libraries. Methods in Molecular Biology 2007, 408:243-255.
15. Hust M., Jostock T., Menzel C., Voedisch B., Mohr A., Brenneis M., Kirsch M.I., Meier D., Dübel S. Single chain Fab (scFab) fragment. BMC Biotechnology 2007, 7:14.
16. Hust M., Meyer T., Voedisch B., Rülker T., Thie H., El-Ghezal A., Kirsch M.I., Schütte M., Helmsing S., Meier D., Schirrmann T., Dübel S. A human scFv antibody generation pipeline for proteome research. Journal of Biotechnology 2011, 152:159-170.
17. Hust M., Steinwand M., Al-Halabi L., Helmsing S., Schirrmann T., Dübel S. Improved microtitre plate production of single chain Fv fragments in Escherichia coli. Nature Biotechnology 2009, 25:424-428.
18. Kellner C., Bleeker W.K., Lammerts van Bueren J.J., Staudinger M., Klausz K., Derer S., Glorius P., Muskulus A., de Goeij B.E.C.G., van de Winkel J.G.J., Parren P.W.H.I., Valerius T., Gramatzki M., Peipp M. Human kappa light chain targeted Pseudomonas exotoxin A-identifying human antibodies and Fab fragments with favorable characteristics for antibody-drug conjugate development. Journal of Immunological Methods 2011, 371:122-133.
19. Kipriyanov S.M., Moldenhauer G., Little M. High level production of soluble single chain antibodies in small-scale Escherichia coli cultures. Journal of Immunological Methods 1997, 200:69-77.
20. Kirsch M., Zaman M., Meier D., Dübel S., Hust M. Parameters affecting the display of antibodies on phage. Journal of Immunological Methods 2005, 301:173-185.
21. Konthur Z., Hust M., Dübel S. Perspectives for systematic in vitro antibody generation. Gene 2005, 364:19-29.
22. Kraft M., Knüpfer U., Wenderoth R., Kacholdt A., Pietschmann P., Hock B., Horn U. A dual expression platform to optimize the soluble production of heterologous proteins in the periplasm of Escherichia coli. Applied Microbiology and Biotechnology 2007, 76:1413-1422.
23. McCafferty J., Griffiths A.D., Winter G., Chiswell D.J. Phage antibodies: filamentous phage displaying antibody variable domains. Nature 1990, 348:552-554.
24. Narayanan N., Khan M., Chou C.P. Enhancing functional expression of Heterologous Burkholderia lipase in Escherichia coli. Molecular Biotechnology 2011, 47:130-143.
25. Plückthun A. Antibody engineering: advances from the use of Escherichia coli expression systems. Biotechnology (N.Y.) 1991, 9:545-551.
26. Sambrook J., Russell D.W. Molecular Cloning: A Laboratory Manual 2001, CSHL Press.
27. Schirrmann T., Al-Halabi L., Dübel S., Hust M. Production systems for recombinant antibodies. Frontiers in Bioscience 2008, 13:4576-4594.
28. Schirrmann T., Büssow K. Transient production of scFv-Fc fusion proteins in mammalian cells. Antibody Engineering 2010, 387-398. Springer, Berlin/Heidelberg. R. Kontermann, S. Dübel (Eds.).
29. Schirrmann T., Hust M. Construction of human antibody gene libraries and selection of antibodies by phage display. Methods in Molecular Biology 2010, 651:177-209.
30. Schlapschy M., Skerra A. Periplasmic chaperones used to enhance functional secretion of proteins in E. coli. Methods in Molecular Biology 2011, 705:211-224.
31. Schütte M., Thullier P., Pelat T., Wezler X., Rosenstock P., Hinz D., Kirsch M.I., Hasenberg M., Frank R., Schirrmann T., Gunzer M., Hust M., Dübel S. Identification of a putative Crf splice variant and generation of recombinant antibodies for the specific detection of Aspergillus fumigatus. PLoS ONE 2009, 4:e6625.
32. Strauch K.L., Johnson K., Beckwith J. Characterization of degP, a gene required for proteolysis in the cell envelope and essential for growth of Escherichia coli at high temperature. Journal of Bacteriology 1989, 171:2689-2696.
33. Taussig M.J., Stoevesandt O., Borrebaeck C.A.K., Bradbury A.R., Cahill D., Cambillau C., de Daruvar A., Dübel S., Eichler J., Frank R., Gibson T.J., Gloriam D., Gold L., Herberg F.W., Hermjakob H., Hoheisel J.D., Joos T.O., Kallioniemi O., Koegl M., Koegll M., Konthur Z., Korn B., Kremmer E., Krobitsch S., Landegren U., van der Maarel S., McCafferty J., Muyldermans S., Nygren P.-A., Palcy S., Plückthun A., Polic B., Przybylski M., Saviranta P., Sawyer A., Sherman D.J., Skerra A., Templin M., Ueffing M., Uhlén M. ProteomeBinders: planning a European resource of affinity reagents for analysis of the human proteome. Nature Methods 2007, 4:13-17.
34. Thie H., Toleikis L., Li J., von Wasielewski R., Bastert G., Schirrmann T., Esteves I.T., Behrens C.K., Fournes B., Fournier N., de Romeuf C., Hust M., Dübel S. Rise and fall of an anti-MUC1 specific antibody. PLoS ONE 2011, 6:e15921.
35. Vedadi M., Lew J., Artz J., Amani M., Zhao Y., Dong A., Wasney G.A., Gao M., Hills T., Brokx S., Qiu W., Sharma S., Diassiti A., Alam Z., Melone M., Mulichak A., Wernimont A., Bray J., Loppnau P., Plotnikova O., Newberry K., Sundararajan E., Houston S., Walker J., Tempel W., Bochkarev A., Kozieradzki I., Edwards A., Arrowsmith C., Roos D., Kain K., Hui R. Genome-scale protein expression and structural biology of Plasmodium falciparum and related Apicomplexan organisms. Molecular and Biochemical Parasitology 2007, 151:100-110.
36. Wezler, X., Hust, M., Helmsing, S., Schirrmann, T., Dübel, S., 2012. Human antibodies targeting CD30+ lymphomas. Human Antibodies, doi:10.3233/HAB-2012-0258.
37. Xu Y., Yasin A., Tang R., Scharer J.M., Moo-Young M., Chou C.P. Heterologous expression of lipase in Escherichia coli is limited by folding and disulfide bond formation. Applied Microbiology and Biotechnology 2008, 81:79-87.