Influence of growth temperature on the production of antibody Fab fragments in different microbes: A host comparative analysis

Biotechnology Progress

Volumn 27, Issue 1, 2011, Pages 38-46

  Dragosits, Martin ^a   Frascotti, Gianni ^b   Bernard Granger, Lise ^c   Vázquez, Felícitas ^d,e   Giuliani, Maria ^f   Baumann, Kristin ^d   Rodríguez Carmona, Escarlata ^d,e   Tokkanen, Jaana ^c   Parrilli, Ermenegilda ^f   Wiebe, Marilyn G ^c   Kunert, Renate ^a   Maurer, Michael ^a,g   Gasser, Brigitte ^a   Sauer, Michael ^a,g   Branduardi, Paola ^b   Pakula, Tiina ^c   Saloheimo, Markku ^c   Penttilä, Merja ^c   Ferrer, Pau ^d   Luisa Tutino, Maria ^f   Villaverde, Antonio ^d,e   Porro, Danilo ^b   Mattanovich, Diethard ^a,g   more..

^a UNIVERSITY OF NATURAL RESOURCES AND LIFE SCIENCES   (Austria)

^b UNIVERSITY OF MILANO BICOCCA   (Italy)

^c VTT TECHNICAL RESEARCH CENTRE OF FINLAND   (Finland)

^d UNIVERSITAT AUTÒNOMA DE BARCELONA   (Spain)

^e BIOMATERIALES Y NANOMEDICINA CIBER BBN   (Spain)

^f UNIVERSITY OF NAPLES FEDERICO II   (Italy)

^g Fachhochschule Campus Wien   (Austria)

Author keywords

Chemostat; Escherichia coli; Pichia pastoris; Pseudoalteromonas haloplanktis; Recombinant protein production; Saccharomyces cerevisiae; Trichoderma reesei

Indexed keywords

PICHIA PASTORIS; PSEUDOALTEROMONAS HALOPLANKTIS; RECOMBINANT PROTEIN PRODUCTION; SACCHAROMYCES CEREVISIAE; TRICHODERMA REESEI;

ANTIBODIES; CHEMOSTATS; CULTIVATION; ESCHERICHIA COLI; FERMENTATION; GROWTH TEMPERATURE; MACHINERY; MICROORGANISMS; PHYSIOLOGICAL MODELS; PHYSIOLOGY;

YEAST;

IMMUNOGLOBULIN F(AB) FRAGMENT;

ARTICLE; BACTERIUM; BIOSYNTHESIS; COMPARATIVE STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; METABOLISM; SPECIES DIFFERENCE; TEMPERATURE; YEAST;

BACTERIA; ENZYME-LINKED IMMUNOSORBENT ASSAY; IMMUNOGLOBULIN FAB FRAGMENTS; SPECIES SPECIFICITY; TEMPERATURE; YEASTS;

ESCHERICHIA COLI; EUKARYOTA; HYPOCREA JECORINA; PICHIA PASTORIS; PROKARYOTA; PSEUDOALTEROMONAS HALOPLANKTIS; SACCHAROMYCES CEREVISIAE;

EID: 79851487300     PISSN: 87567938     EISSN: 15206033     Source Type: Journal    
DOI: 10.1002/btpr.524     Document Type: Article

References (53)

1. Ruiz J, Pinsach J, Álvaro G, González G, de Mas C, Resina D, López-Santín J. Alternative production process strategies in E. coli improving protein quality and downstream yields. Process Biochem. 2009; 44: 1039-1045.
2. Santala V, Lamminmäki U. Production of a biotinylated single-chain antibody fragment in the cytoplasm of Escherichia coli. J Immunol Methods. 2004; 284: 165-175.
3. Volontè F, Marinelli F, Gastaldo L, Sacchi S, Pilone M, Pollegioni L, Molla G. Optimization of glutaryl-7-aminocephalosporanic acid acylase expression in E. coli. Protein Expr Purif. 2008; 61: 131-137.
4. Semba H, Ichige E, Imanaka T, Atomi H, Aoyagi H. Efficient production of active form of recombinant cassava hydroxynitrile lyase using Escherichia coli in low-temperature culture. Appl Microbiol Biotechnol. 2008; 79: 563-569.
5. Kim SS, Kim EK, Rhee JS. Effects of growth rate on the production of Pseudomonas fluorescens lipase during the fed-batch cultivation of Escherichia coli. Biotechnol Prog. 1996; 12: 718-722.
6. Huang D, Shusta EV. Secretion and surface display of green fluorescent protein using the yeast Saccharomyces cerevisiae. Biotechnol Prog. 2005; 21: 349-357.
7. Hackel BJ, Huang D, Bubolz JC, Wang XX, Shusta EV. Production of soluble and active transferrin receptor-targeting single-chain antibody using Saccharomyces cerevisiae. Pharm Res. 2006; 23: 790-797.
8. Shi X, Karkut T, Chamankhah M, Alting-Mees M, Hemmingsen S, Hegedus D. Optimal conditions for the expression of a single-chain antibody (scFv) gene in Pichia pastoris. Protein Expr Purif. 2003; 28: 321-330.
9. Li Z, Xiong F, Lin Q, d'Anjou M, Daugulis A, Yang D, Hew C. Low-temperature increases the yield of biologically active herring antifreeze protein in Pichia pastoris. Protein Expr Purif. 2001; 21: 438-445.
10. Gasser B, Maurer M, Rautio J, Sauer M, Bhattacharyya A, Saloheimo M, Penttilä M, Mattanovich D. Monitoring of transcriptional regulation in Pichia pastoris under protein production conditions. BMC Genomics. 2007; 8: 179.
11. Zhao HL, Xue C, Wang Y, Yao XQ, Liu ZM. Increasing the cell viability and heterologous protein expression of Pichia pastoris mutant deficient in PMR1 gene by culture condition optimization. Appl Microbiol Biotechnol. 2008; 81: 235-241.
12. Dragosits M, Stadlmann J, Albiol J, Baumann K, Maurer M, Gasser B, Sauer M, Altmann F, Ferrer P, Mattanovich D. The effect of temperature on the proteome of recombinant Pichia pastoris. J Proteome Res. 2009; 8: 1380-1392.
13. Swiech K, Rossi N, Astray R, Suazo C. Enhanced production of recombinant rabies virus glycoprotein (rRVGP) by Drosophila melanogaster S2 cells through control of culture conditions. Cytotechnology. 2008; 57: 67-72.
14. Reuveny S, Kim Y, Kemp C, Shiloach J. Effect of temperature and oxygen on cell growth and recombinant protein production in insect cell cultures. Appl Microbiol Biotechnol. 1993; 38: 619-623.
15. Trummer E, Fauland K, Seidinger S, Schriebl K, Lattenmayer C, Kunert R, Vorauer-Uhl K, Weik R, Borth N, Katinger H, Müller D. Process parameter shifting: Part I. Effect of DOT, pH, and temperature on the performance of Epo-Fc expressing CHO cells cultivated in controlled batch bioreactors. Biotechnol Bioeng. 2006; 94: 1033-1044.
16. Bollati-Fogolin M, Forno G, Nimtz M, Conradt HS, Etcheverrigaray M, Kratje R. Temperature reduction in cultures of hGM-CSF-expressing CHO cells: effect on productivity and product quality. Biotechnol Prog. 2005; 21: 17-21.
17. Vera A, Gonzalez-Montalban N, Aris A, Villaverde A. The conformational quality of insoluble recombinant proteins is enhanced at low growth temperatures. Biotechnol Bioeng. 2007; 96: 1101-1106.
18. Martinez-Alonso M, Garcia-Fruitos E, Villaverde A. Yield, solubility and conformational quality of soluble proteins are not simultaneously favored in recombinant Escherichia coli. Biotechnol Bioeng. 2008; 101: 1353-1358.
19. Al-Fageeh MB, Marchant RJ, Carden MJ, Smales CM. The cold-shock response in cultured mammalian cells: harnessing the response for the improvement of recombinant protein production. Biotechnol Bioeng. 2006; 93: 829-835.
20. Regenberg B, Grotkjaer T, Winther O, Fausboll A, Akesson M, Bro C, Hansen LK, Brunak S, Nielsen J. Growth-rate regulated genes have profound impact on interpretation of transcriptome profiling in Saccharomyces cerevisiae. Genome Biol. 2006; 7: R107.
21. Tai S, Daran-Lapujade P, Walsh M, Pronk J, Daran J. Acclimation of Saccharomyces cerevisiae to low temperature: a chemostat-based transcriptome analysis. Mol Biol Cell. 2007; 18: 5100-5112.
22. Gach J, Maurer M, Hahn R, Gasser B, Mattanovich D, Katinger H, Kunert R. High level expression of a promising anti-idiotypic antibody fragment vaccine against HIV-1 in Pichia pastoris. J Biotechnol. 2007; 128: 735-746.
23. Kunert R, Weik R, Ferko B, Stiegler G, Katinger H. Anti-idiotypic antibody Ab2/3H6 mimics the epitope of the neutralizing anti-HIV-1 monoclonal antibody 2F5. AIDS. 2002; 16: 667-668.
24. Demain AL, Vaishnav P. Production of recombinant proteins by microbes and higher organisms. Biotechnol Adv. 2009; 27: 297-306.
25. Parrilli E, Duilio A, Tutino M. Heterologous Protein Expression in Psychrophilic Hosts. Berlin: Springer-Verlag; 2008.
26. Keranen S, Penttila M. Production of recombinant proteins in the filamentous fungus Trichoderma reesei. Curr Opin Biotechnol. 1995; 6: 534-537.
27. Chou C. Engineering cell physiology to enhance recombinant protein production in Escherichia coli. Appl Microbiol Biotechnol. 2007; 76: 521-532.
28. Porro D, Sauer M, Branduardi P, Mattanovich D. Recombinant protein production in yeasts. Mol Biotechnol. 2005; 31: 245-259.
29. Burgess-Brown N, Sharma S, Sobott F, Loenarz C, Oppermann U, Gileadi O. Codon optimization can improve expression of human genes in Escherichia coli: a multi-gene study. Protein Expr Purif. 2008; 59: 94-102.
30. Brake A, Merryweather J, Coit D, Heberlein U, Masiarz F, Mullenbach G, Urdea M, Valenzuela P, Barr P. Alpha-factor-directed synthesis and secretion of mature foreign proteins in Saccharomyces cerevisiae. Proc Natl Acad Sci USA. 1984; 81: 4642-4646.
31. van Dijken JP, Bauer J, Brambilla L, Duboc P, Francois J, Gancedo C, Giuseppin M, Heijnen J, Hoare M, Lange H, Madden E, Niederberger P, Nielsen J, Parrou J, Petit T, Porro D, Reuss M, van Riel N, Rizzi M, Steensma H, Verrips C, Vindeløv J, Pronk J. An interlaboratory comparison of physiological and genetic properties of four Saccharomyces cerevisiae strains. Enzyme Microb Technol. 2000; 26: 706-714.
32. Baumann K, Maurer M, Dragosits M, Cos O, Ferrer P, Mattanovich D. Hypoxic fed-batch cultivation of Pichia pastoris increases specific and volumetric productivity of recombinant proteins. Biotechnol Bioeng. 2008; 100: 177-183.
33. Montenecourt B, Eveleigh D. Selective screening methods for the isolation of high yielding cellulase mutants of Trichoderma reesei on various carbon sources. Adv Chem Ser. 1979; 181: 289-301.
34. Papa R, Rippa V, Sannia G, Marino G, Duilio A. An effective cold inducible expression system developed in Pseudoalteromonas haloplanktis TAC125. J Biotechnol. 2007; 127: 199-210.
35. Rautio J, Kataja K, Satokari R, Penttilä M, Söderlund H, Saloheimo M. Rapid and multiplexed transcript analysis of microbial cultures using capillary electophoresis-detectable oligonucleotide probe pools. J Microbiol Methods. 2006; 65: 404-416.
36. Porro D, Martegani E, Ranzi B, Alberghina L. Oscillations in continuous cultures of budding yeast: a segregated parameter analysis. Biotechnol Bioeng. 1988; 32: 411-417.
37. Rautio J, Bailey M, Kivioja T, Söderlund H, Penttilä M, Saloheimo M. Transcriptional monitoring of steady state and effects of anaerobic phases in chemostat cultures of the filamentous fungus Trichoderma reesei. BMC Genomics. 2006; 7: 247.
38. Gasser B, Maurer M, Gach J, Kunert R, Mattanovich D. Engineering of Pichia pastoris for improved production of antibody fragments. Biotechnol Bioeng. 2006; 94: 353-361.
39. Dragosits M, Stadlmann J, Graf A, Gasser B, Maurer M, Sauer M, Kreil D, Altmann F, Mattanovich D. The response to unfolded protein is involved in osmotolerance of Pichia pastoris. BMC Genomics. 2010; 11: 207.
40. Philips J, Herskowitz I. Osmotic balance regulates cell fusion during mating in Saccharomyces cerevisiae. J Cell Biol. 1997; 138: 961-974.
41. Mack M, Wannemacher M, Hobl B, Pietschmann P, Hock B. Comparison of two expression platforms in respect to protein yield and quality: Pichia pastoris versus Pichia angusta. Protein Expr Purif. 2009; 66: 165-171.
42. Boer E, Steinborn G, Matros A, Mock HP, Gellissen G, Kunze G. Production of interleukin-6 in Arxula adeninivorans, Hansenula polymorpha and Saccharomyces cerevisiae by applying the wide-range yeast vector (CoMed) system to simultaneous comparative assessment. FEMS Yeast Res. 2007; 7: 1181-1187.
43. Jahic M, Wallberg F, Bollok M, Garcia P, Enfors S. Temperature limited fed-batch technique for control of proteolysis in Pichia pastoris bioreactor cultures. Microb Cell Fact. 2003; 2: 6.
44. Mattanovich D, Graf A, Stadlmann J, Dragosits M, Redl A, Maurer M, Kleinheinz M, Sauer M, Altmann F, Gasser B. Genome, secretome and glucose transport highlight unique features of the protein production host Pichia pastoris. Microb Cell Fact. 2009; 8: 29.
45. Kasemets K, Nisamedtinov I, Laht T, Abner K, Paalme T. Growth characteristics of Saccharomyces cerevisiae S288C in changing environmental conditions: auxo-accelerostat study. Antonie Van Leeuwenhoek. 2007; 92: 109-128.
46. Margesin R. Effect of temperature on growth parameters of psychrophilic bacteria and yeasts. Extremophiles. 2009; 13: 257-262.
47. Wittmann C, Weber J, Betiku E, Krömer J, Böhm D, Rinas U. Response of fluxome and metabolome to temperature-induced recombinant protein synthesis in Escherichia coli. J Biotechnol. 2007; 132: 375-384.
48. Lee J, Jeoin Y, Kim D, Seo J, Hur B. Eicosapentaenoic acid (EPA) biosynthetic gene cluster of Shewanella oneidensis MR-1: cloning, heterologous expression, and effects of temperature and glucose on the production of EPA in Escherichia coli. Biotechnol Bioprocess Eng. 2006; 11: 510-515.
49. Simola M, Hänninen A, Stranius S, Makarow M. Trehalose is required for conformational repair of heat-denatured proteins in the yeast endoplasmic reticulum but not for maintenance of membrane traffic functions after severe heat stress. Mol Microbiol. 2000; 37: 42-53.
50. Zancan P, Sola-Penna M. Trehalose and glycerol stabilize and renature yeast inorganic pyrophosphatase inactivated by very high temperatures. Arch Biochem Biophys. 2005; 444: 52-60.
51. Arvas M, Pakula T, Lanthaler K, Saloheimo M, Valkonen M, Suortti T, Robson G, Penttilä M. Common features and interesting differences in transcriptional responses to secretion stress in the fungi Trichoderma reesei and Saccharomyces cerevisiae. BMC Genomics. 2006; 7: 32.
52. Graf A, Gasser B, Dragosits M, Sauer M, Leparc G, Tüchler T, Kreil D, Mattanovich D. Novel insights into the unfolded protein response using Pichia pastoris specific DNA microarrays. BMC Genomics. 2008; 9: 390.
53. Rinas U, Hoffmann F. Selective leakage of host-cell proteins during high-cell-density cultivation of recombinant and non-recombinant Escherichia coli. Biotechnol Prog. 2004; 20: 679-687.