High biological activity of a recombinant protein immobilized onto polystyrene

Biotechnology Journal

Volumn 4, Issue 8, 2009, Pages 1178-1189

  Kumada, Yoichi ^a   Shiritani, Yuki ^a   Hamasaki, Kyoko ^a   Ohse, Takuhito ^b   Kishimoto, Michimasa ^a  

^a KYOTO INSTITUTE OF TECHNOLOGY   (Japan)

^b Enplas Laboratories Inc   (Japan)

Author keywords

Glutathione S transferase; Polystyrene; PS tag; Site specific immobilization

Indexed keywords

ADSORPTION CHARACTERISTIC; ADSORPTION STATE; ALIPHATIC AMINO ACIDS; BINDING PEPTIDE; BIOLOGICAL ACTIVITIES; GLUTATHIONE S-TRANSFERASE; GLUTATHIONE S-TRANSFERASES; HIGH CONCENTRATION; PS-TAG; RECOMBINANT PROTEIN; SITE-SPECIFIC IMMOBILIZATION; SPECIFIC INTERACTION; TARGET PROTEINS; TWEEN-20; WILD TYPES;

ADSORPTION; AMINES; AMINO ACIDS; BODY FLUIDS; COMPETITION; ENZYMES; ORGANIC ACIDS; PEPTIDES; POLYSTYRENES; SYSTEM THEORY;

HYDROPHILICITY;

ARGINYLALANYLISOLEUCYLALANYLARGINYLARGINYLISOLEUCYLARGINYLARGININE; ARGINYLALANYLPHENYLALANYLISOLEUCYLALANYLSERYLARGINYLARGINYLISOLEUCYLLYSYLARGINYLPROLINE; ARGINYLISOLEUCYLISOLEUCYLISOLEUCYLARGINYLARGINYLISOLEUCYLARGINYLARGININE; GLUTATHIONE TRANSFERASE; PEPTIDE; POLYSORBATE 20; POLYSTYRENE; RECOMBINANT PROTEIN; TYPE II SITE SPECIFIC DEOXYRIBONUCLEASE; UNCLASSIFIED DRUG;

ADSORPTION; AFFINITY LABELING; ARTICLE; BIOLOGICAL ACTIVITY; ENZYME IMMOBILIZATION; ENZYME LINKED IMMUNOSORBENT ASSAY; GENE FUSION; HYDROPHILICITY; HYDROPHOBICITY; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL; PROTEIN IMMOBILIZATION; PROTEIN PROTEIN INTERACTION; TISSUE CULTURE;

ADSORPTION; AMINO ACIDS; ANIMALS; BIOTECHNOLOGY; CATTLE; DNA; DOSE-RESPONSE RELATIONSHIP, DRUG; GLUTATHIONE TRANSFERASE; PEPTIDES; POLYSORBATES; POLYSTYRENES; PROTEIN BINDING; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT PROTEINS; SERUM ALBUMIN, BOVINE; SURFACE PROPERTIES;

EID: 70350119793     PISSN: 18606768     EISSN: 18607314     Source Type: Journal    
DOI: 10.1002/biot.200800192     Document Type: Article

References (25)

1. Auerbach, D., Arnoldo, A., Bogdam, B., Fetchko, M., Stagljar, I., Drug discovery using yeast as a model system: A functional genomic and proteomic view. Curr. Proteomics 2005, 2, 1-13.
2. MacBeath, G., Protein microarrays and proteomics. Nat. Genet. 2002, 32, 526-532.
3. MacBeath,G., Schreiber, S. L., Printing proteins as microarrays for high-throughput function determination. Science 2000, 289, 1760-1763.
4. Pillutla, R. C., Fischer, P. B., Blume. A. J., Goldstein, N. I., Target validation and drug discovery using genomic and protein-protein interaction technologies. Expert Opin. Ther. Targets 2002, 6, 517-531.
5. Zhu, H., Klemic, J. F., Chang, S., Bertone, P. et al., Analysis of yeast protein kinases using protein chips. Nat. Genet. 2000, 26, 283-289.
6. Zhu, H., Bilgin, M., Bangham, R., Hall, D. et al., Global analysis of protein activities using proteome chips. Science 2001, 293, 2101-2105.
7. Zhu, H., Snyder, M., Protein chip technology. Curr. Opin. Chem. Biol. 2003, 7, 55-63.
8. Zhang, K., Diehl, M. R., Tirrell, D. A., Artificial polypeptide scaffold for protein immobilization. J. Am. Chem. Soc. 2005, 127, 10136-10137.
9. Butterfield, D. A., Bhattacharyya, D., Daunert, S., Bachas, L., Catalytic biofunctional membranes containing site-specifically immobilized enzyme arrays: A review. J. Membr. Sci. 2001, 181, 29-37.
10. Kreutzberger, J., Protein microarrays: A chance to study microorganisms? Appl. Microbiol. Biotechnol. 2006, 70, 383-390.
11. Hwang, S.Y., Kumada, Y., Seong, G. H., Choo, J.B. et al., Characteristics of a liposome immunoassay on a poly(methyl methacrylate) surface. Anal. Bioanal. Chem. 2007, 389, 2251-2257.
12. Kumada, Y., Tokunaga, Y., Imanaka, H., Imamura, K. et al., Screening and characterization of affinity peptide tags specific to polystyrene supports for the orientated immobilization of proteins. Biotechnol. Prog. 2006, 22, 401-405.
13. Kumada, Y., Katoh, S., Imanaka, H., Imamura, K., Nakanishi, K., Development of a one-step ELISA method using an affinity peptide tag specific to a hydrophilic polystyrene surface. J. Biotechnol. 2007, 127, 288-299.
14. Kumada, Y., Zhao, C., Ishimura, R., Imanaka, H. et al., Protein-protein interaction analysis using an affinity peptide tag and hydrophilic polystyrene plate. J. Biotechnol. 2007, 128, 354-361.
15. Engvall, E., Perlmann, P., Enzyme-linked immunosorbent assay, ELISA. 3. Quantitation of specific antibodies by enzyme labeled anti-immunoglobulin in antigen-coated tubes. J. Immunol. 1972, 109, 129-135.
16. Engvall, E., Jonsson, K., Perlmann, P., Enzyme-linked immunosorbent assay. II. Quantitative assay of protein antigen, immunoglobulin G, by means of enzyme-labelled antigen and antibody-coated tubes. Biochim. Biophys. Acta 1971, 251, 427-434.
17. Pesce, A. J., Ford, D. J., Gaizutis, M., Pollak, V. E., Binding of protein to polystyrene in solid phase immunoassays. Biochim. Biophys. Acta 1977, 492, 399-407.
18. Kumada, Y., Nogami, M., Minami, N., Maehara, M., Katoh, S., Application of protein-coupled liposomes to effective affinity screening from phage library. J. Chromatogr. A 2005, 1080, 22-28.
19. Kumada, Y., Nogami, M., Hasan, F., Terashima, M., Katoh, S., Epitope screening by use of a random peptide-displayed phage library and polyclonal antibody-coupled liposomes. J. Chem. Eng. Jpn. 2006, 39, 1195-1199.
20. Moody, M.D., Van Arsdell, S.W., Murphy, K.P., Orencole, S. F., Burns, C., Array-based ELISAs for high-throughput analysis of human cytokines. BioTechniques 2001, 31, 186-194.
21. Widawski, G., Rawiso, M., Fran&ois, B., Self-organized honeycomb morphology of star-polymer polystyrene films. Nature 1994, 369, 387-389.
22. Ertel, S. I., Ratner, B.D., Horbett, T. A., Radiofrequency plasma deposition of oxygen-containing films on polystyrene and poly(ethylene terephthalate) substrates improves endothelial cell growth. J. Biomed. Mater. Res. 1990, 24, 1637-1659.
23. Ertel, S. I., Ratner, B. D., Horbett, T. A., The adsorption and elutability of albumin, IgG, and fibronectin on radiofrequency plasma deposited polystyrene. J. Colloid Interface Sci. 1991, 147, 433-442.
24. Lhoest, J.-B., Detrait, E., Dewez, J.-L., Van Den Bosch De Aguilar, P., Bertrand, P., A new plasma-based method to promote cell adhesion on micrometric tracks on polystyrene substrates. J. Biomater. Sci. Polym. Edn. 1996, 7, 1039-1054.
25. Teare, D. O. H., Emmison, N., Ton-That, C., Bradley, R. H., Cellular attachment to ultraviolet ozone modified polystyrene surfaces. Langmuir 2000, 16, 2818-2824.