Pichia surface display: A tool for screening single domain antibodies

Methods in Molecular Biology

Volumn 911, Issue , 2012, Pages 125-134

  De Schutter, Kristof ^a   Callewaert, Nico ^a  

^a GHENT UNIVERSITY   (Belgium)

Author keywords

Immune library; Matured library; Pichia pastoris; Surface display

Indexed keywords

ANTIBODY;

ARTICLE; CARBOXY TERMINAL SEQUENCE; FUNGAL GENE; GENE EXPRESSION; NONHUMAN; PICHIA PASTORIS; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DOMAIN; SACCHAROMYCES CEREVISIAE ALPHA AGGLUTININ 1 GENE;

EID: 84934440276     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-61779-968-6_8     Document Type: Article

References (4)

1. Boder ET, Wittrup KD (1997) Yeast surface display for screening combinatorial polypeptide libraries. Nat Biotech 15:553-557
2. Boder ET, Wittrup KD (2000) Yeast surface display for directed evolution of protein expression, affinity, and stability. Methods Enzymol 328:430-444
3. Ryckaert S et al (2010) Isolation of antigen-binding camelid heavy chain antibody fragments (nanobodies) from an immune library displayed on the surface of Pichia pastoris. J Biotech 145:93-98
4. Wu S, Letchworth GJ (2004) High efficiency transformation by electroporation of Pichia pastoris pretreated with lithium acetate and dithiothreitol. Biotechniques 36:152-154