Improved lectin ELISA for glycosylation analysis of biomarkers using PS-tag-fused single-chain Fv

Journal of Immunological Methods

Volumn 385, Issue 1-2, 2012, Pages 15-22

  Kumada, Yoichi ^a   Ohigashi, Yuji ^a   Emori, Yusuke ^a   Imamura, Koji ^b   Omura, Yoshiaki ^b   Kishimoto, Michimasa ^a  

^a KYOTO INSTITUTE OF TECHNOLOGY   (Japan)

^b Medical & Biological Laboratories Co Ltd   (Japan)

Author keywords

CEA; Glyco chain; Lectin ELISA; PS tag; ScFv; Solid phase refolding

Indexed keywords

BIOLOGICAL MARKER; CARCINOEMBRYONIC ANTIGEN; GLYCOPROTEIN; GLYCOSIDE; IMMOBILIZED ANTIBODY; LECTIN; POLYSTYRENE; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY;

ANTIGEN BINDING; ARTICLE; ASSAY; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; DOUBLE ANTIBODY SANDWICH ENZYME LINKED IMMUNOSORBENT ASSAY; ENZYME LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; GLYCOSYLATION; LECTIN ENZYME LINKED IMMUNOSORBENT ASSAY; NONHUMAN; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN FOLDING; PROTEIN PROCESSING; PROTEIN STRUCTURE; SENSITIVITY ANALYSIS;

ANTIBODIES; ANTIBODIES, IMMOBILIZED; BIOLOGICAL MARKERS; CARCINOEMBRYONIC ANTIGEN; CONCANAVALIN A; ENZYME-LINKED IMMUNOSORBENT ASSAY; GLYCOSYLATION; HORSERADISH PEROXIDASE; HUMANS; IMMUNOCONJUGATES; LECTINS; PHYTOHEMAGGLUTININS; POLYSTYRENES; RECOMBINANT PROTEINS; REPRODUCIBILITY OF RESULTS; SEPHAROSE; SINGLE-CHAIN ANTIBODIES;

EID: 84866487628     PISSN: 00221759     EISSN: 18727905     Source Type: Journal    
DOI: 10.1016/j.jim.2012.07.021     Document Type: Article

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