Probing the catalytic mechanism of bovine CD38/NAD +glycohydrolase by site directed mutagenesis of key active site residues

Biochimica et Biophysica Acta - Proteins and Proteomics

Volumn 1844, Issue 7, 2014, Pages 1317-1331

  Kuhn, Isabelle ^a   Kellenberger, Esther ^b   Cakir Kiefer, Céline ^a,c   Muller Steffner, Hélène ^a   Schuber, Francis ^a  

^a Faculté de Pharmacie   (France)

^b Faculté de Pharmacie   (France)

^c UNIVERSITÉ DE LORRAINE   (France)

Author keywords

Active site; Bovine CD38; Catalytic mechanism; Key residue

Indexed keywords

ASPARTIC ACID; CD38 ANTIGEN; FORMIC ACID; GLUTAMIC ACID; GLYCOSIDASE; METHANOL; NICOTINAMIDE; NICOTINAMIDE ADENINE DINUCLEOTIDE; NUCLEOPHILE; RIBOSE; SODIUM AZIDE; TRYPTOPHAN;

ARTICLE; CATALYSIS; CHEMICAL BOND; CONTROLLED STUDY; ENZYME ACTIVE SITE; ENZYME STABILITY; MUTANT; NONHUMAN; PRIORITY JOURNAL; REACTION ANALYSIS; SCISSILE BOND; SITE DIRECTED MUTAGENESIS; SOLVATION; STATIC ELECTRICITY;

ACTIVE SITE; BOVINE CD38; CATALYTIC MECHANISM; KEY RESIDUE;

AMINO ACIDS; ANIMALS; ANTIGENS, CD38; CATALYSIS; CATALYTIC DOMAIN; CATTLE; GLYCOSYLATION; HYDROLYSIS; KINETICS; MAGNETIC RESONANCE SPECTROSCOPY; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; MUTATION; NAD; SUBSTRATE SPECIFICITY;

EID: 84900993089     PISSN: 15709639     EISSN: 18781454     Source Type: Journal    
DOI: 10.1016/j.bbapap.2014.03.014     Document Type: Article

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