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Volumn 122, Issue 8, 2000, Pages 1609-1617

Ricin A-chain: Kinetic isotope effects and transition state structure with stem-loop RNA

Author keywords

[No Author keywords available]

Indexed keywords

RICIN A;

EID: 0034088859     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja992750i     Document Type: Article
Times cited : (77)

References (71)
  • 1
    • 0002169483 scopus 로고
    • Cohen, P., van Heyningen, S., Eds.; Elsevier Biomedical: New York
    • Olsnes, S.; Pihl, A. In Molecular Action of Toxins and Viruses; Cohen, P., van Heyningen, S., Eds.; Elsevier Biomedical: New York, 1982; pp 51-105.
    • (1982) Molecular Action of Toxins and Viruses , pp. 51-105
    • Olsnes, S.1    Pihl, A.2
  • 38
    • 0023129244 scopus 로고
    • Mentch, F.; Parkin, D. W.; Schramm, V. L. Biochemistry 1987, 26, 921-930. Parkin, D. W.; Mentch, F.; Banks, G. A.; Horenstein, B. A.; Schramm, V. L. Biochemistry 1991, 30, 4586-4594.
    • (1987) Biochemistry , vol.26 , pp. 921-930
    • Mentch, F.1    Parkin, D.W.2    Schramm, V.L.3
  • 52
    • 0343174740 scopus 로고    scopus 로고
    • note
    • The double dagger symbol (‡) indicates which step in the reaction is rate-limiting.
  • 53
    • 0343610268 scopus 로고    scopus 로고
    • note
    • In an enzyme catalyzed reaction, the chemical lifetime of an intermediate may not be relevant if the leaving group is prevented from diffusing away by enzyme binding. By analogy to the X-ray crystallographic structures of nucleosidases, nucleotidases, and the structure of RTA, RNA would be expected to bind to RTA with the adenine base buried deeply in the active site cleft and the ribose ring closer to the surface. The ribooxocarbenium is not solvent exposed, since solvent methanol does not react with the intermediate. For these reasons, it is unlikely that the leaving group could diffuse away from the RNA molecule during the lifetime of the intermediate.
  • 70


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.