Transition state analysis of thymidine hydrolysis by human thymidine phosphorylase

Journal of the American Chemical Society

Volumn 132, Issue 38, 2010, Pages 13425-13433

  Schwartz, Phillip A ^a   Vetticatt, Mathew J ^a   Schramm, Vern L ^a  

^a ALBERT EINSTEIN COLLEGE OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACTIVE SITE; ANGIOGENESIS; ANOMERIC CARBON; CATALYTIC BASE; ENERGETIC BARRIERS; HIGH ENERGY; ISOTOPICALLY ENRICHED; KINETIC ISOTOPE EFFECTS; LEAVING GROUPS; NUCLEOPHILIC ATTACK; RATE LIMITING; STEPWISE MECHANISMS; THYMIDINE PHOSPHORYLASE; TRANSITION STATE; TRANSITION STATE STRUCTURE; WATER MOLECULE;

GLUCOSE; ISOTOPES; NUCLEOPHILES; PHOSPHATASES; PHOSPHORYLATION;

DENSITY FUNCTIONAL THEORY;

CARBON; DEOXYRIBOSE; HISTIDINE; NITROGEN; NUCLEOPHILE; THYMIDINE; THYMIDINE PHOSPHORYLASE; WATER;

ARTICLE; CATALYSIS; CHEMICAL BOND; CONFORMATION; DENSITY FUNCTIONAL THEORY; ENERGY; ENERGY TRANSFER; HYDROGEN BOND; HYDROLYSIS;

HUMANS; HYDROLYSIS; MODELS, MOLECULAR; THYMIDINE; THYMIDINE PHOSPHORYLASE;

EID: 77957167437     PISSN: 00027863     EISSN: 15205126     Source Type: Journal    
DOI: 10.1021/ja105041j     Document Type: Article

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1. Abbreviations: dAMP, deoxyadenosine monophosphate; dRib, 2-deoxyribose; dT, thymidine; EIE, equilibrium isotope effect; 5FdU, 2′-deoxy-5- fluorouridine; hTP, human thymidine phosphorylase; KIEs, kinetic isotope effects; PD-ECGF, platelet-derived endothelial cell growth factor; TFT, 5-trifluorothymidine; TPI, thymidine phosphorylase inhibitor (5-chloro-6-[1-(2-iminopyrrolidinyl)methyl]uracil hydrochloride); UDG, uracil DNA gycosylase.
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