Identification of the N-glycosylation sites on recombinant bovine CD38 expressed in Pichia pastoris: Their impact on enzyme stability and catalytic activity

Protein Expression and Purification

Volumn 70, Issue 2, 2010, Pages 151-157

  Muller Steffner, Hélène ^a   Kuhn, Isabelle ^a   Argentini, Manuela ^a   Schuber, Francis ^a  

^a UNIVERSITÉ DE STRASBOURG   (France)

Author keywords

ADP ribosyl cyclase; CD38; Deglycosylated mutants; N Glycosylation sites; NAD+ glycohydrolase; Pichia pastoris

Indexed keywords

CD38 ANTIGEN; GLYCOPEPTIDASE; RECOMBINANT PROTEIN;

AMINO ACID SEQUENCE; ANIMAL; ARTICLE; BIOSYNTHESIS; CATALYSIS; CATTLE; CHEMISTRY; ENZYME STABILITY; GENETICS; GLYCOSYLATION; KINETICS; METABOLISM; PICHIA; SITE DIRECTED MUTAGENESIS;

AMINO ACID SEQUENCE; ANIMALS; ANTIGENS, CD38; CATALYSIS; CATTLE; ENZYME STABILITY; GLYCOSYLATION; KINETICS; MUTAGENESIS, SITE-DIRECTED; PEPTIDE-N4-(N-ACETYL-BETA-GLUCOSAMINYL) ASPARAGINE AMIDASE; PICHIA; RECOMBINANT PROTEINS;

BOVINAE; MAMMALIA; PICHIA PASTORIS;

EID: 76749106494     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2009.10.003     Document Type: Article

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