Next-generation and future DNA sequencing technologies and metagenomics

Metagenomics and its Applications in Agriculture, Biomedicine and Environmental Studies

Volumn , Issue , 2011, Pages 79-106

  Zhang, Peiming ^a   Gu, Jian ^a   He, Jin ^a   Gao, Weimin ^a   Zhang, Weiwen ^a   Lindsay, Stuart ^a   Meldrum, Deirdre R ^a  

^a ARIZONA STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 84895201062     PISSN: None     EISSN: None     Source Type: Book    
DOI: None     Document Type: Chapter

References (121)

1. Aksimentiev, A., Heng, J.B., Timp, G., and Schulten, K. (2004) Microscopic kinetics of DNA translocation through synthetic nanopores. Biophysical Journal 87: 2087-2097.
2. Amit, M. (2003) Dynamics of polynucleotide transport through nanometre-scale pores. Journal of Physics: Condensed Matter: R581.
3. Angly, F.E., Felts, B., Breitbart, M., Salamon, P., Edwards, R.A., Carlson, C. et al. (2006) The marine viromes of four oceanic regions. PLoS Biol 4: e368.
4. Armougom, F., and Raoult, D. (2008) Use of pyrosequencing and DNA barcodes to monitor variations in Firmicutes and Bacteroidetes communities in the gut microbiota of obese humans. BMC Genomics 9: 576.
5. Ashkenasy, N., Sanchez-Quesada, J., Bayley, H., and Ghadiri, M.R. (2005) Recognizing a single base in an individual DNA strand: A step toward DNA sequencing in nanopores. Angewandte Chemie, International Edition 44: 1401-1404.
6. Bains, W., and Smith, C.L. (1988) A novel method for nucleic acid sequence determination. J. Theoret. Biol. 135: 303-307.
7. Bentley, D.R., Balasubramanian, S., Swerdlow, H.P., Smith, G.P., Milton, J., Brown, C.G. et al. (2008a) Accurate whole human genome sequencing using reversible terminator chemistry. Nature 456: 53-59.
8. Bentley, D.R., Balasubramanian, S., Swerdlow, H.P., Smith, G.P., Milton, J., Brown, C.G. et al. (2008b) Accurate whole human genome sequencing using reversible terminator chemistry. Nature 456: 53-59.
9. Blattner, F.R., III, G.P., Bloch, C.A., Perna, N.T., Burland, V., Riley, M. et al. (1997) The complete genome sequence of Escherichia coli K-12. Science 277: 1453-1462.
10. Blow, N. (2008) DNA sequencing: generation next-next. Nature Methods 5: 267-274.
11. Bowers, J., Mitchell, J., Beer, E., Buzby, P.R., Causey, M., Efcavitch, J.W. et al. (2009a) Virtual terminator nucleotides for next-generation DNA sequencing. Nature Methods 6: 593-597.
12. Bowers, R.M., Lauber, C.L., Wiedinmyer, C., Hamady, M., Hallar, A.G., Fall, R. et al. (2009b) Characterization of airborne microbial communities at a high-elevation site and their potential to act as atmospheric ice nuclei. Appl Environ Microbiol 75: 5121-5130.
13. Branton, D., Deamer, D.W., Marziali, A., Bayley, H., Benner, S.A., Butler, T. et al. (2008) The potential and challenges of nanopore sequencing. Nature Biotechnology 26: 1146-1153.
14. Breitbart, M., Hewson, I., Felts, B., Mahaffy, J.M., Nulton, J., Salamon, P., and Rohwer, F. (2003) Metagenomic analysis of an uncultured viral community from human feces. J Bacteriol 185: 6220-6223.
15. Brown, M.V., Philip, G.K., Bunge, J.A., Smith, M.C., Bissett, A., Lauro, F.M. et al. (2009) Microbial community structure in the North Pacific ocean. ISME J. 3: 1374-1386.
16. Butler, T.Z., Pavlenok, M., Derrington, I.M., Niederweis, M., and Gundlach, J.H. (2008) Single-molecule DNA detection with an engineered MspA protein nanopore. Proc. Natl. Acad. Sci. USA 105: 20647-20652.
17. Cardenas, E., and Tiedje, J.M. (2008) New tools for discovering and characterizing microbial diversity. Curr Opin Biotechnol 19: 544-549.
18. Chang, S., He, J., Kibel, A., Lee, M., Sankey, O., Zhang, P., and Lindsay, S. (2009) Tunnelling readout of hydrogen-bonding-based recognition. Nature Nanotechnology 4: 297-301.
19. Cheikh, C., and Koper, G. (2007) Influence of the stick-slip transition on the electrokinetic behavior of nanoporous material. Physica A: Statistical and Theoretical Physics 373: 21-28.
20. Chin, K.J., Sharma, M.L., Russell, L.A., O'Neill, K.R., and Lovley, D.R. (2008) Quantifying expression of a dissimilatory (bi)sulfite reductase gene in petroleum-contaminated marine harbor sediments. Microb Ecol 55: 489-499.
21. Clarke, J., Wu, H.C., Jayasinghe, L., Patel, A., Reid, S., and Bayley, H. (2009) Continuous base identification for single-molecule nanopore DNA sequencing. Nature Nanotechnology 4: 265-270.
22. Cochrane, G., Aldebert, P., Althorpe, N., Andersson, M., Baker, W., Baldwin, A. et al. (2006) EMBL Nucleotide Sequence Database: developments in 2005. Nucleic Acids Research 34: D10-D15.
23. Dinsdale, E.A., Edwards, R.A., Dana Hall1, F.A., Breitbart, M., Brulc, J.M., Furlan, M. et al. (2008) Functional metagenomic profiling of nine biomes. Nature 452: 629-632.
24. Dressman, D., Yan, H., Traverso, G., Kinzler, K.W., and Vogelstein, B. (2003) Transforming single DNA molecules into fluorescent magnetic particles for detection and enumeration of genetic variations.Proc. Natl. Acad. Sci. USA 100: 8817-8822.
25. Drmanac, R., and Crkvenjakov, R. (1987) In. YU, p. 000000570.
26. Drmanac, R., Drmanac, S., Chui, G., Diaz, R., Hou, A., Jin, H. et al. (2002) Sequencing by hybridization (SBH): advantages, achievements, and opportunities. In Advances in Biochemical Engineering/Biotechnology,Vol. 77. Scheper, T. (ed). Berlin Heidelberg: Springer-Verlag pp. 75-100.
27. Edwards, R.A., Rodriguez-Brito, B., Wegley, L., Haynes, M., Breitbart, M., Peterson, D.M. et al. (2006) Using pyrosequencing to shed light on deep mine microbial ecology. BMC Genomics 7: 57.
28. Eid, J., Fehr, A., Gray, J., Luong, K., Lyle, J., Otto, G. et al. (2009) Real-time DNA sequencing from single polymerase polecules. Science 323: 133-138.
29. Fan, R., Karnik, R., Yue, M., Li, D., Majumdar, A., and Yang, P. (2005) DNA Translocation in Inorganic Nanotubes. Nano Letters 5: 1633-1637.
30. Fologea, D., Uplinger, J., Thomas, B., McNabb, D.S., and Li, J. (2005) Slowing DNA translocation in a solid-state nanopore. Nano Letters 5: 1734-1737.
31. Frias-Lopez, J., Shi, Y., Tyson, G.W., Coleman, M.L., Schuster, S.C., Chisholm, S.W., and DeLong, E.F. (2008) Microbial community gene expression in ocean surface waters. Proc. Natl. Acad. Sci. USA 105: 3805-3810.
32. Gaidos, E., Marteinsson, V., Thorsteinsson, T., Jóhannesson, T., Rúnarsson, Á.R., Stefansson, A. et al. (2009) An oligarchic microbial assemblage in the anoxic bottom waters of a volcanic subglacial lake. ISME J 3: 486-497.
33. Gilbert, J.A., Field, D., Huang, Y., Edwards, R., Li, W., Gilna, P., and Joint, I. (2008) Detection of large numbers of novel sequences in the metatranscriptomes of complex marine microbial communities. PLoS ONE 3: e3042.
34. Giovannoni, S.J., Britschgi, T.B., Moyer, C.L., and Field, K.G. (1990) Genetic diversity in Sargasso Sea bacterioplankton. Nature 345: 60-63.
35. Goff, S.A., Ricke, D., Lan, T.-H., Presting, G., Wang, R., Dunn, M. et al. (2002) A draft sequence of the rice genome (Oryza sativa L. ssp. japonica). Science 296: 92-100.
36. Goffeau, A., Barrell, B.G., Bussey, H., Davis, R.W., Dujon, B., Feldmann, H. et al. (1996) Life with 6000 genes Science 274: 546, 563-567.
37. Gupta, P.K. (2008) Single-molecule DNA sequencing technologies for future genomics research. Trends in Biotechnology 26: 602-611.
38. Hallam, S.J., Putnam, N., Preston, C.M., Detter, J.C., Rokhsar, D., Richardson, P.M., and DeLong, E.F. (2004) Reverse methanogenesis: testing the hypothesis with environmental genomics. Science 305: 1457-1462.
39. Handelsman, J., Rondon, M.R., Brady, S.F., Clardy, J., and Goodman, R.M. (1998) Molecular biological access to the chemistry of unknown soil microbes: a new frontier for natural products. Chem Biol 5: R245-R249.
40. Hardin, S.H. (2008) Real-Time DNA sequencing. In Next-Generation Genome Sequencing. Janitz, M. (ed). Weinheim: Wiley-Blackwell, pp. 97-101.
41. Hoffmann, C., Hill, D.A., Minkah, N., Kirn, T., Troy, A., Artis, D., and Bushman, F. (2009) Community-wide response of gut microbiota to enteropathogenic Citrobacter infection revealed by deep sequencing. Infect Immun.: published online
42. Hoshino, T., Furukawa, K., Tsuneda, S., and Inamori, Y. (2007) RNA microarray for estimating rel-ative abundance of 16S rRNA in microbial communities. J Microbiol Methods 69: 406-410.
43. Hubbell, E. (2001) Multiplex sequencing by hybridization. Journal of Computational Biology 8: 141-149.
44. Hugenholtz, P., and Tyson, G.W. (2008) Metagenomics. Nature 455: 481-483.
45. The Arabidopsis Genome Initiative. (2000) Analysis of the genome sequence of the flowering plant Arabidopsis thaliana. Nature 408: 796-815.
46. Jones, R.T., Robeson, M.S., Lauber, C.L., Hamady, M., Knight, R., and Fierer, N. (2009) A comprehensive survey of soil acidobacterial diversity using pyrosequencing and clone library analyses. ISME J 3: 442-453.
47. Kang, X.-f., Gu, L.-Q., Cheley, S., and Bayley, H. (2005) Single protein pores containing molecular adapters at high temperatures. Angew. Chem. Int. Ed. 44: 1495-1499.
48. Kasianowicz, J.J., ERIC, E.B., Branton, D., and Deamer, D.W. (1996) Characterization of individual polynucleotide molecules using a membrane channel. Proc. Natl. Acad. Sci. USA 93: 13770-13773.
49. Kasianowicz, J.J., Henrickson, S.E., Weetall, H.H., and Robertson, B. (2001) Simultaneous multianalyte detection with a nanometer-scale pore. Analytical Chemistry 73: 2268-2272.
50. Korlach, J., Bibillo, A., Wegener, J., Peluso, P., Pham, T.T., Park, I. et al. (2008) Long processive enzymatic DNA synthesis using 100% dye-labelled terminal phosphate-linked nucleotides Nucleosides, Nucleotides and Nucleic Acids 27: 1072-1083.
51. Lander, E.S. et al. (2001) Initial sequencing and analysis of the human genome. Nature 409: 860-921.
52. Lauber, C.L., Hamady, M., Knight, R., and Fierer, N. (2009) Pyrosequencing-based assessment of soil pH as a predictor of soil bacterial community structure at the continental scale. Appl Environ Microbiol 75: 5111-5120.
53. Leininger, S., Urich, T., Schloter, M., Schwark, L., Qi, J., Nicol, G.W. et al. (2006) Archaea predominate among ammonia-oxidizing prokaryotes in soils. Nature 442: 806-809.
54. Li, J., Stein, D., McMullan, C., Branton, D., Aziz, M.J., and Golovchenko, J.A. (2001) Ion-beam sculpting at nanometre length scales. Nature 412: 166-169.
55. Liang, X., and Chou, S.Y. (2008) Nanogap detector inside nanofluidic channel for fast real-time label-free DNA analysis. Nano Letters 8: 1472-1476.
56. Linsday, S.M., and Philipp, M. (1991) Can the scanning tunneling microscope aequence DNA? GATA 8: 8-13.
57. Liu, H., He, J., Tang, J., Liu, H., Pang, P., Cao, D., Krstic, P., Joseph, S., Lindsay, S., Nuckolls, C., (2010) Translocation of Single-Stranded DNA Through Single-Walled Carbon Nanotubes. Science 327: 64-67.
58. Lysov, Y.P., Khorlin, A.A., Khrapko, K.R., Shick, V.V., Florentiev, V.L., and Mirzabekov, A.D. (1988) Proc. USSR Acad. Sci 303: 1508-1511.
59. Maglia, G., Henricus, M., Wyss, R., Li, Q., Cheley, S., and Bayley, H. (2009) DNA strands from denatured duplexes are translocated through engineered protein nanopores at alkaline pH. Nano Letters 9: 3831-3836.
60. Margulies, M., Egholm, M., Altman, W.E., Attiya, S., Bader, J.S., Bemben, L.A. et al. (2005a) Genome sequencing in microfabricated high-density picolitre reactors. Nature 437: 376-380.
61. Margulies, M., Egholm, M., Altman, W.E., Attiya, S., Bader, J.S., Bemben, L.A. et al. (2005b) Genome sequencing in microfabricated high-density picolitre reactors. Nature 437: 376-380.
62. Maxam, A.M., and Gilbert, W. (1977) A new method for sequencing DNA. Proc. Nati. Acad. Sci. USA 74: 560-564.
63. Meldrum, D.R. (2000a) Automation for genomics: Part 1, preparation for sequencing. Genome Research 10: 1081-1092.
64. Meldrum, D.R. (2000b) Automation for genomics: Part 2, sequencers, microarrays, and future trends. Genome Research, 10: 1288-1303.
65. Meldrum, D.R. (2001) Sequencing genomes and beyond. Science 292: 515-517.
66. Meldrum, D.R., and Holl, M.R. (2002 ) Microscale bioanalytical systems Science 297: 1197-1198.
67. Meller, A., Nivon, L., Brandin, E., Golovchenko, J., and Branton, D. (2000) Rapid nanopore discrimination between single polynucleotide molecules. Proc. Nalt. Acad. Aci. USA 97: 1079-1084.
68. Miller, S.R., Strong, A.L., Jones, K.L., and Ungerer, M.C. (2009) Bar-coded pyrosequencing reveals shared bacterial community properties along the temperature gradients of two alkaline hot springs in Yellowstone National Park. Appl Environ Microbiol 75: 4565-4572.
69. Moran, M.A. (2009) Metatranscriptomics: eavesdropping on Complex microbial communities. Microbes 4: 329-335.
70. Muthukumar, M., and Kong, C.Y. (2006) Simulation of polymer translocation through protein channels. Proc. Nati. Acad. Sci. USA 103: 5273-5278.
71. Pace, N.R. (1997) A molecular view of microbial diversity and the biosphere. Science 276: 734-740.
72. Parro, V., Moreno-Paz, M., and Gonzalez-Toril, E. (2009) Analysis of environmental transcriptomes by DNA microarrays. Env Microbiol 9: 453-464.
73. Pe'er, I., Arbili, N., and Shamir, R. (2002) A computational method for resequencing long DNA targets by universal oligonucleotide arrays. Proc. Nati. Acad. Sci. USA 99: 15492-15496 .
74. Pevzner, P.A., and Lipshutz, R.J. (1994) Towards DNA-sequencing by hybridization. In 19th Symp. on Mathem. Found. of Comp. Sci., pp. 141-258.
75. Pevzner, P.A., Lysov, Y.P., Khrapko, K.R., Belyavsky, A.V., Florentiev, V.L., and Mirzabekov, A.D. (1991) Improved chips for sequencing by hybridization. J. Biomolecul. Struct. & Dynamics 9: 399-410.
76. Pihlak, A., Bauren, G., Hersoug, E., Loennerberg, P., Metsis, A., and Linnarsson, S. (2008) Rapid genome sequencing with short universal tiling probes. Nature biotechnology 26: 676-684.
77. Pinkel, D., and Albertson, D.G. (2005) Array comparative genomic hybridization and its applications in cancer. Nature genetics supplement 37: S11-S17.
78. Poretsky, R.S., Hewson, I., Sun, S., Allen, A.E., Zehr, J.P., and Moran, M.A. (2009) Comparative day/night metatranscriptomic analysis of microbial communities in the North Pacific subtropical gyre. Environ Microbiol. 11: 1358-1375.
79. Poretsky, R.S., Bano, N., Buchan, A., LeCleir, G., Kleikemper, J., Pickering, M. et al. (2005) Analysis of microbial gene transcripts in environmental samples. Appl Env Microbiol 71: 4121-4126.
80. Preparata, F.P., Frieze, A.M., and Upfal, E. (1999) On the power of universal bases in sequencing by hybridization. In 3rd Int. Conf. Computational Molecular Biology,, pp. 295-301.
81. Price, L.B., Liu1, C.M., Melendez, J.H., Frankel, Y.M., Engelthaler, D., Aziz, M. et al. (2009) Community analysis of chronic wound bacteria using 16S rRNA gene-based pyrosequencing: impact of diabetes and antibiotics on chronic wound microbiota. PLoS One 4: e6462.
82. Pushkarev, D., Neff, N.F., and Quake, S.R. (2009) Single-molecule sequencing of an individual human genome. Nature Biotechnology.
83. Qi, W., Nong, G., Preston, J.F., Ben-Ami, F., and Ebert, D. (2009) Comparative metagenomics of Daphnia symbionts. BMC Genomics 10: 172.
84. Rhee, M., and Burns, M.A. (2007) Nanopore sequencing technology: nanopore preparations. Trends in Biotechnology 25: 174-181.
85. Rich, V.I., Konstantinidis, K., and DeLong, E.F. (2008) Design and testing of 'genome-proxy' microarrays to profile marine microbial communities. Environ Microbiol 10: 506-521.
86. Roesch, L.F., Fulthorpe, R.R., Riva, A., Casella5, G., Hadwin, A.K., Kent, A.D. et al. (2007) Pyrosequencing enumerates and contrasts soil microbial diversity. ISME J 1: 283-290.
87. Roesch, L.F., Lorca, G.L., Casella, G., Giongo, A., Naranjo, A., Pionzio, A.M. et al. (2009) Culture-independent identification of gut bacteria correlated with the onset of diabetes in a rat model. ISME J 3: 536-548.
88. Sanapareddy, N., Hamp, T.J., Gonzalez, L.C., Hilger, H.A., Fodor, A.A., and Clinton, S.M. (2009) Molecular diversity of a North Carolina wastewater treatment plant as revealed by pyrosequencing. Appl Environ Microbiol 75: 1688-1696.
89. Sanger, F., and Coulson, A.R. (1975) A rapid method for determining sequences in DNA by primed synthesis with DNA polymerase. J. Mol. Biol. 94: 441-448.
90. Sanger, F., Nicklen, S., and Coulson, A.R. (1977) DNA sequencing with chain-terminating inhibitors. Proc. Nati. Acad. Sci. USA 74: 5463-5467.
91. Sessler, A.M., and Dawson, J. (1993) Method For Sequencing DNA Base Pairs. In. US, pp. 5,270,214.
92. Shendure, J., and Ji, H. (2008) Next-generation DNA sequencing. Nature Biotechnology 26: 1135-1145.
93. Shendure, J., Porreca, G.J., Reppas, N.B., Lin, X., McCutcheon, J.P., Rosenbaum, A.M. et al. (2005) Accurate multiplex polony sequencing of an evolved bacterial genome. Science 309: 1728-1732.
94. Shi, Y., Tyson, G.W., and DeLong, E.F. (2009) Metatranscriptomics reveals unique microbial small RNAs in the ocean's water column. Nature 459: 266-269.
95. Shiu, S.H., and Borevitz, J.O. (2008) The next generation of microarray research: applications in evolutionary and ecological genomics. Heredity 100: 100: 141-149.
96. Sigalov, G., Comer, J., Timp, G., and Aksimentiev, A. (2007) Detection of DNA sequences using an alternating electric field in a nanopore capacitor. Nano Letters 8: 56-63.
97. Singh, J., Behal, A., Singla, N., Joshi, A., Birbian, N., Singh, S. et al. (2009) Metagenomics: Concept, methodology, ecological inference and recent advances. Biotechnol J 4: 480-494.
98. Smeets, R.M.M., Dekker, N.H., and Dekker, C. (2009) Low-frequency noise in solid-state nanopores. Nanotechnology: 095501.
99. Soni, G.V., and Meller, A. (2007) Progress toward ultrafast DNA sequencing using solid-state nanopores. Clin Chem 53: 1996-2001.
100. Southern, E.M. (1988) International Patent Application PCT GB.
101. Southern, E.M., Maskos, U., and Elder, J.K. (1992) Analyzing and comparing nucleic acid sequences by hybridization to arrays of oligonucleotides: evaluation using experimental models. Genomics 13: 1008-1017.
102. Stewart, F.J., and Cavanaugh, C.M. (2009 ) Pyrosequencing analysis of endosymbiont population structure: co-occurrence of divergent symbiont lineages in a single vesicomyid host clam. Environ Microbiol 11: 2136-2147.
103. Tanaka, H., and Kawai, T. (2009) Partial sequencing of a single DNA molecule with a scanning tunnelling microscope. Nature nanotechnology 4: 518-522.
104. Thomas, W.K., and Glover, W. (2008) Direc Sequencing by TEM of Z-sunstituted DNA molecules. In Next-Generation Genome Sequencing Towards Personalized Medicine. Janitz, M. (ed). Weinheim: Wiley-Blackwell, pp. 103-116.
105. Thurber, R.V., Willner-Hall, D., Rodriguez-Mueller, B., Desnues, C., Edwards, R.A., Angly, F. et al. (2009) Metagenomic analysis of stressed coral holobionts. Environ Microbiol 11: 2148-2163.
106. Tyson, G.W., Chapman, J., Hugenholtz, P., Allen, E.E., Ram, R.J., Richardson, P.M. et al. (2004) Community structure and metabolism through reconstruction of microbial genomes from the environment. Nature 428 37-43.
107. Urich, T., Lanzén, A., Qi, J., Huson, D.H., Schleper, C., and Schuster, S.C. (2008) Simultaneous assessment of soil microbial community structure and function through analysis of the meta-transcriptome. PLoS ONE 3: e2527.
108. Venter, J.C. et al. (2001) The sequence of the human genome. Science 291: 1304-1351.
109. Venter, J.C., Remington, K., Heidelberg, J.F., Halpern, A.L., Rusch, D., Eisen, J.A. et al. (2004) Environmental genome shotgun sequencing of the Sargasso Sea. Science 304: 66-74.
110. Ward, D.M. (2006) Microbial diversity in natural environments: focusing on fundamental questions. Antonie Van Leeuwenhoek 90: 309-324.
111. Warnecke, F., and Hugenholtz, P. (2007) Building on basic metagenomics with complementary technologies. Genome Biol 8: 231.
112. Waterman, M.S. (1995) Introduction to computational biology: maps, sequences and genomes: Chapman & Hall.
113. Waterston, R.H., Lindblad-Toh, K., Birney, E., Rogers, J., Abril, J.F., Agarwal, P. et al. (2002) Initial sequencing and comparative analysis of the mouse genom. Nature 420: 520-562.
114. Wegley, L., Edwards, R., Rodriguez-Brito, B., Liu, H., and Rohwer, F. (2007) Metagenomic analysis of the microbial community associated with the coral Porites astreoides. Environ Microbial 9: 2707-2719.
115. Wu, M.Y., Smeets, R.M.M., Zandbergen, M., Ziese, U., Krapf, D., Batson, P.E. et al. (2009) Control of shape and material composition of solid-state nanopores. Nano Letters 9: 479-484.
116. Yeh, I.-C., and Hummer, G. (2004) Nucleic acid transport through carbon nanotube membranes. Proc. Natl. Acad. Sci. USA 101: 12177-12182.
117. Yinghong, X., Yong, K., Soh, A.K., and Huajian, G. (2007) Electric field-induced translocation of single-stranded DNA through a polarized carbon nanotube membrane. The Journal of Chemical Physics 127: 225101.
118. Yu, J., Hu, S., Wang, J., Wong, G.K.-S., Li, S., Liu, B. et al. (2002) A draft sequence of the rice genome (Oryza sativa L. ssp. indica). Science 296: 79-92.
119. Zhang, H., DiBaise, J.K., Zuccolo, A., Kudrna, D., Braidotti, M., Yu, Y. et al. (2009) Human gut microbiota in obesity and after gastric bypass. Proc Natl Acad Sci USA 106: 2365-2370.
120. Zhou, J. (2003) Microarrays for bacterial detection and microbial community analysis Curr Opin Microbiol 6: 288-294.
121. Zwolak, M., and Di Ventra, M. (2005) Electronic signature of DNA nucleotides via transverse transport. Nano Letters 5: 421-424.