Molecular biology: Accurate multiplex polony sequencing of an evolved bacterial genome

Science

Volumn 309, Issue 5741, 2005, Pages 1728-1732

  Shendure, Jay ^a   Porreca, Gregory J ^a   Reppas, Nikos B ^a   Lin, Xiaoxia ^a   McCutcheon, John P ^b   Rosenbaum, Abraham M ^a   Wang, Michael D ^a   Zhang, Kun ^a   Mitra, Robi D ^b   Church, George M ^a  

^a HARVARD MEDICAL SCHOOL   (United States)

^b WASHINGTON UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

COLOR IMAGE PROCESSING; DNA; ENZYMES; ESCHERICHIA COLI; GENES; IMAGING TECHNIQUES;

CHAIN REACTION; DNA SEQUENCING AUTOMATION; EPIFLUORESCENCE MICROSCOPES; FOUR-COLOR IMAGING;

MOLECULAR BIOLOGY;

BIOCHEMISTRY; GENETICS;

ARTICLE; BACTERIAL GENOME; DNA SEQUENCE; EPIFLUORESCENCE MICROSCOPE; ESCHERICHIA COLI; GENE AMPLIFICATION; GENE TECHNOLOGY; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; ALGORITHM; AUTOMATION; COST; ECONOMICS; FLUORESCENCE MICROSCOPY; GEL; GENE LIBRARY; GENETICS; INSTRUMENTATION; METABOLISM; METHODOLOGY; MOLECULAR EVOLUTION; MUTATION; NUCLEIC ACID HYBRIDIZATION; POINT MUTATION;

ESCHERICHIA COLI;

ACRYLIC ACID RESIN; BACTERIAL DNA; FLUORESCENT DYE; MICROSPHERE; POLYACRYLAMIDE GELS; POLYDEOXYRIBONUCLEOTIDE SYNTHASE; PRIMER DNA;

ACRYLIC RESINS; ALGORITHMS; AUTOMATION; COSTS AND COST ANALYSIS; DNA LIGASES; DNA PRIMERS; DNA, BACTERIAL; ESCHERICHIA COLI; EVOLUTION, MOLECULAR; FLUORESCENT DYES; GELS; GENE LIBRARY; GENOME, BACTERIAL; MICROSCOPY, FLUORESCENCE; MICROSPHERES; MUTATION; NUCLEIC ACID HYBRIDIZATION; POINT MUTATION; POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS, DNA;

EID: 24644462173     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.1117389     Document Type: Article

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28. For advice, encouragement, and technical assistance, we are deeply indebted to J. Zhu, S. Douglas, J. Chou, J. Aach, M. Nikku, A. Lee, N. Novikov, and M. Wright (Church Lab); A. Blanchard, C. Costa, H. Ebling, J. Ichikawa, J. Malek, P. McEwan, K. McKeman, A. Sheridan, and D. Smith (Agencourt); S. Skiena (SUNY-Stony Brook) C. Felts (RPI); R. Fincher (Alcott); D. Focht (Bioptechs); and M. Hotfelder and J. Feng (Washington University). We thank B. Vogelstein, J. Edwards, and their groups for assistance with emulsion PCR. This work was supported by the National Human Genome Research Institute-Centers of Excellence in Cenomic Science and U.S. Department of Energy-Genomes to Life grants.