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Volumn 105, Issue 52, 2008, Pages 20647-20652

Single-molecule DNA detection with an engineered MspA protein nanopore

Author keywords

Bio nanotechnology; DNA sequencing; Protein engineering

Indexed keywords

BACTERIAL PROTEIN; PROTEIN MSPA; UNCLASSIFIED DRUG;

EID: 58549116498     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.0807514106     Document Type: Article
Times cited : (390)

References (36)
  • 1
    • 0030465241 scopus 로고    scopus 로고
    • Characterization of individual polynucleotide molecules using a membrane channel
    • Kasianowicz JJ, Brandin E, Branton D, Deamer DW (1996) Characterization of individual polynucleotide molecules using a membrane channel. Proc Natl Acad Sci USA 93:13770-13773.
    • (1996) Proc Natl Acad Sci USA , vol.93 , pp. 13770-13773
    • Kasianowicz, J.J.1    Brandin, E.2    Branton, D.3    Deamer, D.W.4
  • 3
    • 0031194564 scopus 로고    scopus 로고
    • Designed protein pores as components for biosensors
    • Braha O, et al. (1997) Designed protein pores as components for biosensors. Chem Biol 4:497-505.
    • (1997) Chem Biol , vol.4 , pp. 497-505
    • Braha, O.1
  • 4
    • 0035849885 scopus 로고    scopus 로고
    • Ion-beam sculpting at nanometer-length scales
    • Li J, et al. (2001) Ion-beam sculpting at nanometer-length scales. Nature 412:166-169.
    • (2001) Nature , vol.412 , pp. 166-169
    • Li, J.1
  • 5
    • 16644398754 scopus 로고    scopus 로고
    • Sizing DNA using a nanometer-diameter pore
    • Heng JB, et al. (2004) Sizing DNA using a nanometer-diameter pore. Biophys J 87:2905-2911.
    • (2004) Biophys J , vol.87 , pp. 2905-2911
    • Heng, J.B.1
  • 7
    • 0032762996 scopus 로고    scopus 로고
    • Microsecond time-scale discrimination among polycytidylic acid, polyadenylic acid, and polyuridylic acid as homopolymers or as segments within single RNA molecules
    • Akeson M, Branton D, Kasianowicz JJ, Brandin E, Deamer DW (1999) Microsecond time-scale discrimination among polycytidylic acid, polyadenylic acid, and polyuridylic acid as homopolymers or as segments within single RNA molecules. Biophys J 77:3227-3233.
    • (1999) Biophys J , vol.77 , pp. 3227-3233
    • Akeson, M.1    Branton, D.2    Kasianowicz, J.J.3    Brandin, E.4    Deamer, D.W.5
  • 9
    • 0035107270 scopus 로고    scopus 로고
    • Rapid discrimination among individual DNA hairpin molecules at single-nucleotide resolution using an ion channel
    • Vercoutere W, et al. (2001) Rapid discrimination among individual DNA hairpin molecules at single-nucleotide resolution using an ion channel. Nat Biotechnol 19:248-252.
    • (2001) Nat Biotechnol , vol.19 , pp. 248-252
    • Vercoutere, W.1
  • 10
    • 0036931449 scopus 로고    scopus 로고
    • Probing distance and electrical potential within a protein pore with tethered DNA
    • Howorka S, Bayley H (2002) Probing distance and electrical potential within a protein pore with tethered DNA. Biophys J 83:3202-3210.
    • (2002) Biophys J , vol.83 , pp. 3202-3210
    • Howorka, S.1    Bayley, H.2
  • 12
    • 14844318136 scopus 로고    scopus 로고
    • Nanopore unzipping of individual DNA hairpin molecules
    • Mathe J, Visram H, Viasnoff V, Rabin Y, Meller A (2004) Nanopore unzipping of individual DNA hairpin molecules. Biophys J 87:3205-3212.
    • (2004) Biophys J , vol.87 , pp. 3205-3212
    • Mathe, J.1    Visram, H.2    Viasnoff, V.3    Rabin, Y.4    Meller, A.5
  • 14
    • 3042736845 scopus 로고    scopus 로고
    • A nanosensor for transmembrane capture and identification of single nucleic acid molecules
    • Nakane J, Wiggin M, Marziali A (2004) A nanosensor for transmembrane capture and identification of single nucleic acid molecules. Biophys J 87:615-621.
    • (2004) Biophys J , vol.87 , pp. 615-621
    • Nakane, J.1    Wiggin, M.2    Marziali, A.3
  • 15
    • 24644451580 scopus 로고    scopus 로고
    • Orientation discrimination of single-stranded DNA inside the α-hemolysin membrane channel
    • Mathe J, Aksimentiev A, Nelson DR, Schulten K, Meller A (2005) Orientation discrimination of single-stranded DNA inside the α-hemolysin membrane channel. Proc Natl Acad Sci USA 102:12377-12382.
    • (2005) Proc Natl Acad Sci USA , vol.102 , pp. 12377-12382
    • Mathe, J.1    Aksimentiev, A.2    Nelson, D.R.3    Schulten, K.4    Meller, A.5
  • 16
    • 36049035070 scopus 로고    scopus 로고
    • Ionic current blockades from DNA and RNA molecules in the α-hemolysin nanopore
    • Butler TZ, Gundlach JH, Troll M (2007) Ionic current blockades from DNA and RNA molecules in the α-hemolysin nanopore. Biophys J 93:3229-3240.
    • (2007) Biophys J , vol.93 , pp. 3229-3240
    • Butler, T.Z.1    Gundlach, J.H.2    Troll, M.3
  • 17
    • 34247536747 scopus 로고    scopus 로고
    • Single-molecule analysis of DNA-protein complexes using nanopores
    • Hornblower B, et al. (2007) Single-molecule analysis of DNA-protein complexes using nanopores. Nat Methods 4:315-317.
    • (2007) Nat Methods , vol.4 , pp. 315-317
    • Hornblower, B.1
  • 18
    • 35648976000 scopus 로고    scopus 로고
    • Stochastic detection of motor protein-RNA complexes by single-channel current recording
    • Astier Y, Kainov DE, Bayley H, Tuma R, Howorka S (2007) Stochastic detection of motor protein-RNA complexes by single-channel current recording. Chemphyschem 8:2189-2194.
    • (2007) Chemphyschem , vol.8 , pp. 2189-2194
    • Astier, Y.1    Kainov, D.E.2    Bayley, H.3    Tuma, R.4    Howorka, S.5
  • 19
    • 35948947371 scopus 로고    scopus 로고
    • Sequence-specific detection of individual DNA polymerase complexes in real time using a nanopore
    • Benner S, et al. (2007) Sequence-specific detection of individual DNA polymerase complexes in real time using a nanopore. Nat Nanotechnol 2:718-724.
    • (2007) Nat Nanotechnol , vol.2 , pp. 718-724
    • Benner, S.1
  • 20
    • 44049102228 scopus 로고    scopus 로고
    • Outer membrane protein G: Engineering a quiet pore for biosensing
    • Chen M, Khalid S, Sansom MS, Bayley H (2008) Outer membrane protein G: Engineering a quiet pore for biosensing. Proc Natl Acad Sci USA 105:6272-6277.
    • (2008) Proc Natl Acad Sci USA , vol.105 , pp. 6272-6277
    • Chen, M.1    Khalid, S.2    Sansom, M.S.3    Bayley, H.4
  • 21
    • 14844290867 scopus 로고    scopus 로고
    • Recognizing a single base in an individual DNA strand: A step toward DNA sequencing in nanopores
    • Ashkenasy N, Sanchez-Quesada J, Bayley H, Ghadiri MR (2005) Recognizing a single base in an individual DNA strand: A step toward DNA sequencing in nanopores. Angew Chem Int Ed Engl 44:1401-1404.
    • (2005) Angew Chem Int Ed Engl , vol.44 , pp. 1401-1404
    • Ashkenasy, N.1    Sanchez-Quesada, J.2    Bayley, H.3    Ghadiri, M.R.4
  • 22
    • 32244449078 scopus 로고    scopus 로고
    • Toward single-molecule DNA sequencing: Direct identification of ribonucleoside and deoxyribonucleoside 5′-monophosphates by using an engineered protein nanopore equipped with a molecular adapter
    • Astier Y, Braha O, Bayley H (2006) Toward single-molecule DNA sequencing: Direct identification of ribonucleoside and deoxyribonucleoside 5′-monophosphates by using an engineered protein nanopore equipped with a molecular adapter. J Am Chem Soc 128:1705-1710.
    • (2006) J Am Chem Soc , vol.128 , pp. 1705-1710
    • Astier, Y.1    Braha, O.2    Bayley, H.3
  • 23
    • 33745774997 scopus 로고    scopus 로고
    • Direct force measurements on DNA in a solid-state nanopore
    • Keyser UF, et al. (2006) Direct force measurements on DNA in a solid-state nanopore. Nat Phys 2:473-477.
    • (2006) Nat Phys , vol.2 , pp. 473-477
    • Keyser, U.F.1
  • 24
    • 38349169632 scopus 로고    scopus 로고
    • A single-molecule nanopore device detects DNA polymerase activity with single-nucleotide resolution
    • Cockroft SL, Chu J, Amorin M, Ghadiri MR (2008) A single-molecule nanopore device detects DNA polymerase activity with single-nucleotide resolution. J Am Chem Soc 130:818-820.
    • (2008) J Am Chem Soc , vol.130 , pp. 818-820
    • Cockroft, S.L.1    Chu, J.2    Amorin, M.3    Ghadiri, M.R.4
  • 25
    • 0141677886 scopus 로고    scopus 로고
    • Mycobacterial porins: New channel proteins in unique outer membranes
    • Niederweis M (2003) Mycobacterial porins: New channel proteins in unique outer membranes. Mol Microbiol 49:1167-1177.
    • (2003) Mol Microbiol , vol.49 , pp. 1167-1177
    • Niederweis, M.1
  • 26
    • 0035028415 scopus 로고    scopus 로고
    • MspA provides the main hydrophilic pathway through the cell wall of Mycobacterium smegmatis
    • Stahl C, et al. (2001) MspA provides the main hydrophilic pathway through the cell wall of Mycobacterium smegmatis. Mol Microbiol 40:451-464.
    • (2001) Mol Microbiol , vol.40 , pp. 451-464
    • Stahl, C.1
  • 27
    • 1242352009 scopus 로고    scopus 로고
    • The structure of a mycobacterial outer-membrane channel
    • Faller M, Niederweis M, Schulz GE (2004) The structure of a mycobacterial outer-membrane channel. Science 303:1189-1192.
    • (2004) Science , vol.303 , pp. 1189-1192
    • Faller, M.1    Niederweis, M.2    Schulz, G.E.3
  • 28
    • 0037424352 scopus 로고    scopus 로고
    • The core of the tetrameric mycobacterial porin MspA is an extremely stable β-sheet domain
    • Heinz C, Engelhardt H, Niederweis M (2003) The core of the tetrameric mycobacterial porin MspA is an extremely stable β-sheet domain. J Biol Chem 278:8678-8685.
    • (2003) J Biol Chem , vol.278 , pp. 8678-8685
    • Heinz, C.1    Engelhardt, H.2    Niederweis, M.3
  • 29
    • 33646845510 scopus 로고    scopus 로고
    • Topology of the porin MspA in the outer membrane of Mycobacterium smegmatis
    • Mahfoud M, Sukumaran S, Hulsmann P, Grieger K, Niederweis M (2006) Topology of the porin MspA in the outer membrane of Mycobacterium smegmatis. J Biol Chem 281:5908-5915.
    • (2006) J Biol Chem , vol.281 , pp. 5908-5915
    • Mahfoud, M.1    Sukumaran, S.2    Hulsmann, P.3    Grieger, K.4    Niederweis, M.5
  • 30
    • 0032881596 scopus 로고    scopus 로고
    • Cloning of the mspA gene encoding a porin from Mycobacterium smegmatis
    • Niederweis M, et al. (1999) Cloning of the mspA gene encoding a porin from Mycobacterium smegmatis. Mol Microbiol 33:933-945.
    • (1999) Mol Microbiol , vol.33 , pp. 933-945
    • Niederweis, M.1
  • 31
    • 27444443072 scopus 로고    scopus 로고
    • The growth rate of Mycobacterium smegmatis depends on sufficient porin-mediated influx of nutrients
    • Stephan J, et al. (2005) The growth rate of Mycobacterium smegmatis depends on sufficient porin-mediated influx of nutrients. Mol Microbiol 58:714-730.
    • (2005) Mol Microbiol , vol.58 , pp. 714-730
    • Stephan, J.1
  • 32
    • 0038752648 scopus 로고    scopus 로고
    • Polymer translocation through a long nanopore
    • Slonkina E, Kolomeisky AB (2003) Polymer translocation through a long nanopore. J Chem Phys 118:7112-7118.
    • (2003) J Chem Phys , vol.118 , pp. 7112-7118
    • Slonkina, E.1    Kolomeisky, A.B.2
  • 33
    • 0030447720 scopus 로고    scopus 로고
    • Structure of staphylococcal α-hemolysin, a heptameric transmembrane pore
    • Song L, et al. (1996) Structure of staphylococcal α-hemolysin, a heptameric transmembrane pore. Science 274:1859-1866.
    • (1996) Science , vol.274 , pp. 1859-1866
    • Song, L.1
  • 34
    • 0030843812 scopus 로고    scopus 로고
    • CCR: A rapid and simple approach for mutation detection
    • Bi W, Stambrook PJ (1997) CCR: A rapid and simple approach for mutation detection. Nucleic Acids Res 25:2949-2951.
    • (1997) Nucleic Acids Res , vol.25 , pp. 2949-2951
    • Bi, W.1    Stambrook, P.J.2
  • 35
    • 0034307651 scopus 로고    scopus 로고
    • Selective extraction and purification of a mycobacterial outer membrane protein
    • Heinz C, Niederweis M (2000) Selective extraction and purification of a mycobacterial outer membrane protein. Anal Biochem 285:113-120.
    • (2000) Anal Biochem , vol.285 , pp. 113-120
    • Heinz, C.1    Niederweis, M.2
  • 36
    • 1542779619 scopus 로고    scopus 로고
    • Purification of porins from Mycobacterium smegmatis
    • Heinz C, Roth E, Niederweis M (2003) Purification of porins from Mycobacterium smegmatis. Methods Mol Biol 228:139-150.
    • (2003) Methods Mol Biol , vol.228 , pp. 139-150
    • Heinz, C.1    Roth, E.2    Niederweis, M.3


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.