Long, processive enzymatic DNA synthesis using 100% dye-labeled terminal phosphate-linked nucleotides

Nucleosides, Nucleotides and Nucleic Acids

Volumn 27, Issue 9, 2008, Pages 1072-1082

  Korlach, Jonas ^a   Bibillo, Arek ^a   Wegener, Jeffrey ^a   Peluso, Paul ^a   Pham, Thang T ^a   Park, Insil ^a   Clark, Sonya ^a   Otto, Geoff A ^a   Turner, Stephen W ^a  

^a PACIFIC BIOSCIENCES   (United States)

Author keywords

DNA; dNTP; Fluorescence; Sequencing applications

Indexed keywords

DEOXYRIBONUCLEOSIDE TRIPHOSPHATE; DNA POLYMERASE; FLUORESCENT DYE;

ARTICLE; BACTERIOPHAGE; CONJUGATION; COUPLING FACTOR; DNA SEQUENCE; DNA STRAND; DNA SYNTHESIS; FLUORESCENCE; POLYMERIZATION;

EID: 49749112881     PISSN: 15257770     EISSN: 15322335     Source Type: Journal    
DOI: 10.1080/15257770802260741     Document Type: Article

References (2)

1. Blanco, L.; Salas, M. Relating structure to function in phi29 DNA polymerase. J. Biol. Chem. 1996, 271, 8509-8512.
2. de Vega, M.; Lazaro, J.M.; Salas, M.; Blanco, L. Primer-terminus stabilization at the 3′-5′ exonuclease active site of phi29 DNA polymerase. Involvement of two amino acid residues highly conserved in proofreading DNA polymerases. EMBO J. 1996, 15, 1182-1192.