Exploiting the metabolism of PYC expressing HEK293 cells in fed-batch cultures

Journal of Biotechnology

Volumn 169, Issue 1, 2014, Pages 63-70

  Vallée, Cédric ^a   Durocher, Yves ^b   Henry, Olivier ^a  

^a ÉCOLE POLYTECHNIQUE DE MONTRÉAL   (Canada)

^b NATIONAL RESEARCH COUNCIL CANADA   (Canada)

Author keywords

Fed batch; HEK293 cells; Pyruvate carboxylase; Recombinant protein

Indexed keywords

ANIMAL CELL LINES; FED BATCHES; FED-BATCH CULTURES; GLUCOSE CONCENTRATION; HEK293 CELLS; PROCESS ENHANCEMENTS; PYRUVATE CARBOXYLASE; RECOMBINANT YEAST;

AUTOMATION; BATCH DATA PROCESSING; CELLS; CONTROL; CYTOLOGY; RECOMBINANT PROTEINS;

BATCH CELL CULTURE;

GLUCOSE; PYRUVATE CARBOXYLASE; RECOMBINANT ALPHA2B INTERFERON;

ANIMAL CELL; ARTICLE; BATCH PROCESS; CELL CLONE; CELL DENSITY; CELL STRAIN HEK293; CONTROLLED STUDY; ENZYME METABOLISM; FED BATCH CULTURE; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; YEAST;

FED-BATCH; HEK293 CELLS; PYRUVATE CARBOXYLASE; RECOMBINANT PROTEIN;

BATCH CELL CULTURE TECHNIQUES; BIOREACTORS; HEK293 CELLS; HUMANS; PYRUVATE CARBOXYLASE; RECOMBINANT PROTEINS;

EID: 84889579877     PISSN: 01681656     EISSN: 18734863     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2013.11.002     Document Type: Article

References (47)

1. Aehle M., Schaepe S., Kuprijanov A., Simutis R., Lubbert A. Simple and efficient control of CHO cell cultures. Journal of Biotechnology 2011, 153:56-61.
2. Altamirano C., Illanes A., Becerra S., Cairo J.J., Godia F. Considerations on the lactate consumption by CHO cells in the presence of galactose. J Biotechnol 2006, 125:547-556.
3. Altamirano C., Paredes C., Cairo J.J., Godia F. Improvement of CHO cell culture medium formulation: simultaneous substitution of glucose and glutamine. Biotechnol Prog 2000, 16:69-75.
4. Altamirano C., Paredes C., Illanes A., Cairo J.J., Godia F. Strategies for fed-batch cultivation of t-PA producing CHO cells: substitution of glucose and glutamine and rational design of culture medium. J Biotechnol 2004, 110:171-179.
5. Andersen D.C., Goochee C.F. The effect of ammonia on the O-linked glycosylation of granulocyte colony-stimulating factor produced by chinese hamster ovary cells. Biotechnol Bioeng 1995, 47:96-105.
6. Bell S.L., Bebbington C., Scott M.F., Wardell J.N., Spier R.E., Bushell M.E., Sanders P.G. Genetic engineering of hybridoma glutamine metabolism. Enzyme and Microbial Technology 1995, 17:98-106.
7. Bibila T.A., Robinson D.K. In Pursuit of the Optimal Fed-Batch Process for Monoclonal Antibody Production. Biotechnol. Prog. 1995, 11:1-13.
8. Borys M.C., Linzer D.I.H., Papoutsakis E.T. Ammonia affects the glycosylation patterns of recombinant mouse placental lactogen-I by chinese hamster ovary cells in a pH-dependent manner. Biotechnology and Bioengineering 1994, 43:505-514.
9. Chee Furng Wong D., Tin Kam Wong K., Tang Goh L., Kiat Heng C., Gek Sim Yap M. Impact of dynamic online fed-batch strategies on metabolism, productivity and N-glycosylation quality in CHO cell cultures. Biotechnology and Bioengineering 2005, 89:164-177.
10. Chen K., Liu Q., Xie L., Sharp P.A., Wang D.I. Engineering of a mammalian cell line for reduction of lactate formation and high monoclonal antibody production. Biotechnol Bioeng 2001, 72:55-61.
11. Cruz H.J., Freitas C.M., Alves P.M., Moreira J.L., Carrondo M.J.T. Effects of ammonia and lactate on growth, metabolism, and productivity of BHK cells. Enzyme and Microbial Technology 2000, 27:43-52.
12. Cruz H.J., Moreira J.L., Carrondo M.J.T. Metabolic shifts by nutrient manipulation in continuous cultures of BHK cells. Biotechnology and Bioengineering 1999, 66:104-113.
13. Dowd J.E., Weber I., Rodriguez B., Piret J.M., Kwok K.E. Predictive control of hollow-fiber bioreactors for the production of monoclonal antibodies. Biotechnology and Bioengineering 1999, 63:484-492.
14. Elias C.B., Carpentier E., Durocher Y., Bisson L., Wagner R., Kamen A. Improving glucose and glutamine metabolism of human HEK 293 and Trichoplusia ni insect cells engineered to express a cytosolic pyruvate carboxylase enzyme 2003, 90-97. American Chemical Society. 1 ed.
15. Fogolín M.B., Wagner R., Etcheverrigaray M., Kratje R. Impact of temperature reduction and expression of yeast pyruvate carboxylase on hGM-CSF-producing CHO cells. Journal of Biotechnology 2004, 109:179-191.
16. Gagnon M., Hiller G., Luan Y.-T., Kittredge A., DeFelice J., Drapeau D. High-End pH-controlled delivery of glucose effectively suppresses lactate accumulation in CHO Fed-batch cultures. Biotechnology and Bioengineering 2011, 108:1328-1337.
17. Gálvez J., Lecina M., Solà C., Cairó J.J., Gòdia F. Optimization of HEK-293S cell cultures for the production of adenoviral vectors in bioreactors using on-line OUR measurements. Journal of Biotechnology 2012, 157:214-222.
18. Gambhir A., Korke R., Lee J.C., Fu P.C., Europa A., Hu W.S. Analysis of cellular metabolism of hybridoma cells at distinct physiological states. Journal of Bioscience and Bioengineering 2003, 95:317-327.
19. Gawlitzek M., Estacio M., Furch T., Kiss R. Identification of cell culture conditions to control N-glycosylation site-occupancy of recombinant glycoproteins expressed in CHO cells. Biotechnol Bioeng 2009, 103:1164-1175.
20. Gawlitzek M., Ryll T., Lofgren J., Sliwkowski M.B. Ammonium alters N-glycan structures of recombinant TNFR-IgG: degradative versus biosynthetic mechanisms. Biotechnol Bioeng 2000, 68:637-646.
21. Genzel Y., Ritter J.B., Konig S., Alt R., Reichl U. Substitution of glutamine by pyruvate to reduce ammonia formation and growth inhibition of mammalian cells. Biotechnol Prog 2005, 21:58-69.
22. Gorfien S.F., Paul W., Judd D., Tescione L., Jayme D.W. Optimized nutrient additives for fed-batch cultures. Biopharm International 2003, 16:34-40.
23. Hammett K., Kuchibhatla J., Hunt C., Holdread S., Brooks J. Developing Chemically Defined Media Through DOE: Complete Optimization with Increased Protein Production in Less than 8 Months. Cell Technology for Cell Products. 2007, 683-691. Springer Netherlands. R. Smith (Ed.).
24. Henry O., Durocher Y. Enhanced glycoprotein production in HEK-293 cells expressing pyruvate carboxylase. Metabolic Engineering 2011, 13:499-507.
25. Hu S., Deng L., Wang H., Zhuang Y., Chu J., Zhang S., Li Z., Guo M. Bioprocess development for the production of mouse-human chimeric anti-epidermal growth factor receptor vIII antibody C12 by suspension culture of recombinant Chinese hamster ovary cells. Cytotechnology 2011, 63:247-258.
26. Huang Y.M., Hu W., Rustandi E., Chang K., Yusuf-Makagiansar H., Ryll T. Maximizing productivity of CHO cell-based fed-batch culture using chemically defined media conditions and typical manufacturing equipment. Biotechnol Prog 2010, 26:1400-1410.
27. Irani N., Beccaria A.J., Wagner R. Expression of recombinant cytoplasmic yeast pyruvate carboxylase for the improvement of the production of human erythropoietin by recombinant BHK-21 cells. Journal of Biotechnology 2002, 93:269-282.
28. Irani N., Wirth M., Van Den Heuvel J., Wagner R. Improvement of the primary metabolism of cell cultures by introducing a new cytoplasmic pyruvate carboxylase reaction. Biotechnology and Bioengineering 1999, 66:238-246.
29. Jeong D.-w., Kim T.S., Lee J.W., Kim K.T., Kim H.J., Kim I.-H., Kim I.Y. Blocking of Acidosis-Mediated Apoptosis by a Reduction of Lactate Dehydrogenase Activity through Antisense mRNA Expression. Biochemical and Biophysical Research Communications 2001, 289:1141-1149.
30. Jiang K.D., Geng Z., Casnocha S., Xiao Z., Gorfien S., Jacobia S.J. Fed-Batch Cell Culture Process Optimization. BioProcess International 2012, 10:40-45.
31. Johnston M.J., Frahm G., Li X., Durocher Y., Hefford M.A. O-linked glycosylation leads to decreased thermal stability of interferon alpha 2b as measured by two orthogonal techniques. Pharm Res 2011, 28:1661-1667.
32. Kim S., Lee G. Down-regulation of lactate dehydrogenase-A by siRNAs for reduced lactic acid formation of Chinese hamster ovary cells producing thrombopoietin. Applied Microbiology and Biotechnology 2007, 74:152-159.
33. Kim S.H., Lee G.M. Functional expression of human pyruvate carboxylase for reduced lactic acid formation of Chinese hamster ovary cells (DG44). Appl Microbiol Biotechnol 2007, 76:659-665.
34. Lao M.S., Toth D. Effects of ammonium and lactate on growth and metabolism of a recombinant Chinese hamster ovary cell culture. Biotechnol. Prog. 1997, 13:688-691.
35. Loignon M., Perret S., Kelly J., Boulais D., Cass B., Bisson L., Afkhamizarreh F., Durocher Y. Stable high volumetric production of glycosylated human recombinant IFNalpha2b in HEK293 cells. BMC Biotechnol 2008, 8:65.
36. Lu F., Toh P.C., Burnett I., Li F., Hudson T., Amanullah A., Li J. Automated dynamic fed-batch process and media optimization for high productivity cell culture process development. Biotechnology and Bioengineering 2013, 110:191-205.
37. Maranga L., Goochee C.F. Metabolism of PER.C6 cells cultivated under fed-batch conditions at low glucose and glutamine levels. Biotechnology and Bioengineering 2006, 94:139-150.
38. Ozturk S.S., Riley M.R., Palsson B.O. Effects of ammonia and lactate on hybridoma growth, metabolism, and antibody production. Biotechnol Bioeng 1992, 39:418-431.
39. Pacis E., Vijayasankaran N., Li J., Gawlitzek M., Amanullah A., Li F. Systematic Approaches to Develop Chemically Defined Cell Culture Feed Media. Biopharm International 2010, 23. 22-24,26-28,30,32.
40. Paredes C., Prats E., Cairó J.J., Azorín F., Cornudella L., Gòdia F. Modification of glucose and glutamine metabolism in hybridoma cells through metabolic engineering. Cytotechnology 1999, 30:85-93.
41. Spens E., Häggström L. Defined protein and animal component-free NS0 fed-batch culture. Biotechnology and Bioengineering 2007, 98:1183-1194.
42. Wlaschin K.F., Hu W.S. Fedbatch culture and dynamic nutrient feeding. Cell Culture Engineering. 2006, 43-74. Springer, New York. W.S. Hu, T. Scheper (Eds.).
43. Yang M., Butler M. Effects of ammonia on CHO cell growth, erythropoietin production, and glycosylation. Biotechnology and Bioengineering 2000, 68:370-380.
44. Yang M., Butler M. Effects of ammonia and glucosamine on the heterogeneity of erythropoietin glycoforms. Biotechnol Prog 2002, 18:129-138.
45. Zhang H.F., Wang H.B., Liu M., Zhang T., Zhang J., Wang X.J., Xiang W.S. Rational development of a serum-free medium and fed-batch process for a GS-CHO cell line expressing recombinant antibody. Cytotechnology 2013, 65:363-378.
46. Zhou W., Hu W.S. On-line characterization of a hybridoma cell culture process. Biotechnol Bioeng 1994, 44:170-177.
47. Zhou W.C., Rehm J., Europa A., Hu W.S. Alteration of mammalian cell metabolism by dynamic nutrient feeding. Cytotechnology 1997, 24:99-108.