Optimization of HEK-293S cell cultures for the production of adenoviral vectors in bioreactors using on-line OUR measurements

Journal of Biotechnology

Volumn 157, Issue 1, 2012, Pages 214-222

  Galvez J ^a   Lecina, M ^a   Sola C ^a   Cairo J J ^a   Godia F ^a  

^a UNIVERSITAT AUTÒNOMA DE BARCELONA   (Spain)

Author keywords

Adenoviral vectors production; Dynamic nutrient feeding; HEK 293S cells; Infection protocol; On line monitoring; OUR; Perfusion culture

Indexed keywords

ADENOVIRAL VECTORS; BATCH CULTURE; CELL ACTIVITY; CELL DENSITY; CULTURE MEDIUM; DYNAMIC METHOD; FEEDING RATE; FEEDING STRATEGIES; INFECTION PROTOCOL; NUTRIENT LIMITATIONS; ONLINE MONITORING; OUR; OXYGEN UPTAKE RATES; PERFUSION CULTURES; PROCESS EVOLUTION; SPECIFIC PRODUCTIVITY; STIRRED TANK BIOREACTORS; TANGENTIAL FLOW; VIRAL PARTICLES;

BIOREACTORS; CELLS; FEEDING; GENE THERAPY; NUTRIENTS; OPTIMIZATION; PROCESS MONITORING; PRODUCTIVITY;

BATCH CELL CULTURE;

ADENOVIRUS VECTOR;

ADENOVIRUS 5; ARTICLE; CELL ACTIVITY; CELL CULTURE; CELL DENSITY; CELL SPECIFICITY; CHEMICAL PARAMETERS; CULTURE MEDIUM; CULTURE OPTIMIZATION; FED BATCH CULTURE; FILTRATION; HUMAN; HUMAN CELL; INFORMATION PROCESSING; METHODOLOGY; NONHUMAN; NUTRIENT; OXYGEN UPTAKE RATE; PRIORITY JOURNAL; STIRRED REACTOR; VIRUS PARTICLE;

ADENOVIRIDAE; BIOREACTORS; CELL COUNT; CELL SURVIVAL; CULTURE MEDIA; GENETIC VECTORS; GLUCOSE; HEK293 CELLS; HUMANS; OXYGEN; PERFUSION; VIRION; VIRUS CULTIVATION;

EID: 84855247115     PISSN: 01681656     EISSN: 18734863     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2011.11.007     Document Type: Article

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