Ammonium alters N-glycan structures of recombinant TNFR-IgG: Degradative versus biosynthetic mechanisms

Biotechnology and Bioengineering

Volumn 68, Issue 6, 2000, Pages 637-646

  Gawlitzek, Martin ^a   Ryll, Thomas ^a   Lofgren, Jim ^a   Sliwkowski, Mary B ^a  

^a GENENTECH INC   (United States)

Author keywords

Ammonium; Chinese hamster ovary (CHO); N glycosylation; Recombinant glycoprotein

Indexed keywords

AMMONIUM COMPOUNDS; BIOSYNTHESIS; CELL CULTURE; DEGRADATION; ENZYMES; MONOMERS; PROTEINS;

AMMONIUM; CHINESE HAMSTER OVARY; GLYCOSYLATION; RECOMBINANT GLYCOPROTEIN;

BIOTECHNOLOGY;

AMMONIA; GLYCAN; GLYCOPROTEIN; HYBRID PROTEIN; N ACETYLNEURAMINIC ACID;

ARTICLE; CARBOHYDRATE METABOLISM; CELL COMPARTMENTALIZATION; CHO CELL; COVALENT BOND; ENERGY METABOLISM; GLYCOSYLATION; IMMUNOGENICITY; PROTEIN DEGRADATION;

ANIMALS; BETA-GALACTOSIDASE; BIOREACTORS; BIOTECHNOLOGY; CHO CELLS; CRICETINAE; GLYCOSYLATION; GOLGI APPARATUS; HYDROGEN-ION CONCENTRATION; IMMUNOGLOBULIN GAMMA-CHAINS; IMMUNOGLOBULIN HEAVY CHAINS; N-ACETYLLACTOSAMINE SYNTHASE; NEURAMINIDASE; NUCLEOTIDES; POLYSACCHARIDES; QUATERNARY AMMONIUM COMPOUNDS; RECEPTORS, TUMOR NECROSIS FACTOR; RECOMBINANT FUSION PROTEINS; RNA, MESSENGER; SIALYLTRANSFERASES;

CRICETINAE; CRICETULUS GRISEUS;

EID: 0034691229     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0290(20000620)68:6<637::AID-BIT6>3.0.CO;2-C     Document Type: Article

References (42)

1. Andersen DC, Goochee CF. 1995. The effect of ammonia on the O-linked glycosylation of granulocyte colony-stimulating factor produced by Chinese hamster ovary cells. Biotechnol Bioeng 47:96-105.
2. Anumula KR. 1995. Rapid quantitative determination of sialic acids in glycoproteins by high-performance liquid chromatography with a sensitive fluorescence detection. Anal Biochem 230:24-30.
3. Barasch J, Kiss B, Prince A, Saiman L, Gruenert D, Al-Awqati Q. 1991. Defective acidification of intracellular organelles in cystic fibrosis. Nature 352:70-73.
4. Borys MC, Linzer DIH, Papoutsakis T. 1994. Ammonia affects the glycosylation patterns of recombinant mouse placental lactogen-I by Chinese hamster ovary cells in pH-dependent manner. Biotechnol Bioeng 43:505-514.
5. Chamow SM, Ashkenazi A. 1996. Immunoadhesins: Principles and applications. Trends Biotechnol 14:52-60.
6. Ferrari J, Harris R, Warner TG. 1994. Cloning and expression of a soluble sialidase from Chinese hamster ovary cells: Sequence alignment similarities to bacterial sialidases. Glycobiology 4:367-373.
7. Forman LW, Thomas BD, Jacobson FS. 1991. On-line monitoring and control of fermentation processes by flow-injection analysis. Anal Chim Acta 249:101-111.
8. 15N from ammonium into the N-linked oligosaccharides of an immunoadhesin glycoprotein expressed in Chinese hamster ovary cells. Glycobiology 9:125-131.
9. Gawlitzek M, Valley U, Wagner R. 1998. Ammonium ion and glucosamine dependent increase of oligosaccharide complexity in recombinant glycoproteins secreted from cultivated BHK-21 cells. Biotechnol Bioeng 57:518-512.
10. Goergen JL, Marc A, Engasser JM. 1993. Determination of cell lysis and death kinetics in continuous hybridoma cultures from the measurement of lactate dehydrogenase release. Cytotechnology 11:189-195.
11. Goochee CF, Gramer MJ, Andersen DC, Bahr JB, Rasmussen JR. 1991. The oligosaccharides of glycoproteins: Bioprocess factors affecting oligosaccharide structure and their effect on glycoprotein properties. Bio/Technology 9:1347-1355.
12. Gramer MJ, Goochee CF. 1993. Glycosidase activities in Chinese hamster ovary cell lysate and cell culture supernatant. Biotechnol Prog 9: 366-373.
13. Gramer MJ, Goochee CF, Chock VY, Brousseau DT, Sliwkowski MB. 1995. Removal of sialic acid from glycoprotein in CHO cell culture supernatant by action of an extracellular CHO cell sialidase. Bio/ Technology 13:692-698.
14. Grammatikos SI, Valley U, Nimtz M, Conradt HS, Wagner R. 1998. Intracellular UDP-N-acetylhexosamine pool affects N-glycan complexity - a mechanism of ammonium action on protein glycosylation. Biotechnol Prog 14:410-419.
15. Gross HJ, Sticher U, Brossmer R. 1990. A highly sensitive fluorometric assay for sialyltransferase activity using CMP-9-fluoresceinyl-NeuAc as donor. Anal Biochem 186:127-134.
16. Hasilik A, Neufeld EF. 1980a. Biosynthesis of lysosomal enzymes in fibroblasts. J Biol Chem 255:4937-4945.
17. Hasilik A, Neufeld EF. 1980b. Biosynthesis of lysosomal enzymes in fibroblasts. J Biol Chem 255:4946-4950.
18. Jenkins N, Parekh RB, James DC. 1996. Getting the glycosylation right: Implications for the biotechnology industry. Nature Biotechnol 14: 975-981.
19. Kaufman RJ, Sharp PA. 1982. Amplification and expression of sequences cotransfected with a modular dihydrofolate reductase complementary DNA gene. J Mol Biol 159:601-621.
20. Kim JH, Lingwood CA, Williams DB, Furuya W, Manolson MF, Grinstein S. 1996. Dynamic measurement of the pH of the Golgi complex in living cells using retrograde transport of the verotoxin receptor. J Cell Biol 134:1387-1399.
21. Moore A, Mercer J, Dutina G, Donahue CJ, Bauer KD, Mather JP, Etcheverry T, Ryll T. 1997. Effects of temperature shift on cell cycle, apoptosis and nucleotide pools in CHO batch cultures. Cytotechnology 23:47-54.
22. Papac DI, Wong A, Jones AJS. 1996. Analysis of acidic oligosaccharides and glycopeptides by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Anal Chem 68:3215-3223.
23. Pels Rijcken WR, Overdijk B, Van Den Eijnden DH, Ferwerda W. 1995. The effect of increasing nucleotide-sugar concentrations on the incorporation of sugars into glycoconjugates in rat hepatocytes. Biochem J 305:865-870.
24. Potier M, Mameli L, Beliste M, Dallaire L, Melancon SB. 1979. Fluorometric assay of neuraminidase with sodium 4-methylumbelliferyl-α-D-N-acetylneuraminate substrate. Anal Biochem 94:287-296.
25. Reitzer LJ, Wice BM, Kennell D. 1979. Evidence that glutamine, not sugar, is the major energy source for cultured HeLa cells. J Biol Chem 254:2669-2676.
26. Ryll T. 1999. Stategies to reduce sialidase impact on product quality in CHO production cultures. Oral presentation at the 16th ESACT meeting, Lugano, Switzerland.
27. Ryll T, Valley U, Wagner R. 1994. Biochemistry of growth inhibition by ammonium ions in mammalian cells. Biotechnol Bioeng 44:184-193.
28. Ryll T, Wagner R. 1991. Improved ion pair high-performance liquid chromatographic method for the quantification of a wide variety of nucleotides and sugar-nucleotides in animal cells. J Chromatogr 570:77-88.
29. Ryll T, Wagner R. 1992. Intracellular ribonucleotide pools as a tool for monitoring the physiological state of in vitro cultivated mammalian cells during production process. Biotechnol Bioeng 40:934-946.
30. Schneider M, Marison IW, von Stockar U. 1996. The importance of ammonia in mammalian cell cultures. J Biotechnol 46:161-185.
31. Seksck O, Biwersi J, Verkman AS. 1995. Direct measurement of trans-Golgi pH in living cells and regulation by second messengers. J Biol Chem 270:4967-4970.
32. Stryer L. 1995. Mannose 6-phosphate targets lysosomal enzymes to their destination, 4th ed. New York: W.H. Freeman and Co.
33. Tapper H, Sundler R. 1990. Role of lysosomal and cytosolic pH in the regulation of macrophage lysosomal enzyme secretion. Biochem J 272:407-414.
34. Thorens B, Vassalli P. 1986. Chloroquine and ammonium chloride prevent terminal glycosylation of immunoglobulins in plasma cells without affecting secretion. Nature 321:618-620.
35. Urlaub G, Chasin LA. 1980. Isolation of Chinese hamster cell mutants deficient in dihydrofolate reductase activity. Proc Natl Acad Sci USA 77:4216-4220.
36. Varki A. 1993. Biological roles of oligosaccharides: All of the theories are correct. Glycobiology 3:97-130.
37. von Figura K, Hasilik A. 1986. Lysosomal enzymes and their receptors. Ann Rev Biochem 55:167-193.
38. Waldmann BC, Rudnik G. 1990. UDP-GIcNAc transport across the Golgi membrane: Electroneutral exchange for dianionic UMP. Biochem 29:44-42.
39. Zanghi JA, Mendoza TP, Knop RH, Miller WM. 1998a. Ammonia inhibits neural cell adhesion molecule polysialylation in Chinese hamster ovary and small cell lung cancer cells. J Cell Physiol 177:248-263.
40. Zanghi JA, Mendoza TP, Schmelzer AE, Knop RH, Miller WM. 1998b. Role of nucleotide sugar pools in the inhibition of NCAM polysialylation by ammonia. Biotechnol Prog 14:834-844.
41. Zatta PF, Nyame K, Cormier MJ, Mattox SA, Prieto PA, Smith DF, Cummings RD. 1991. A solid-phase assay for β-1,4-galactosyltransferase activity in human serum using recombinant aequorin. Anal Biochem 194:185-191.
42. Zielke HR, Zielke CL, Ozand PT. 1984. Glutamine: A major energy source for cultured mammalian cells. Fed Proc 43:121-125.