Stable high volumetric production of glycosylated human recombinant IFNalpha2b in HEK293 cells

BMC Biotechnology

Volumn 8, Issue , 2008, Pages

  Loignon, Martin ^a   Perret, Sylvie ^a   Kelly, John ^a   Boulais, Denise ^a   Cass, Brian ^a   Bisson, Louis ^a   Afkhamizarreh, Fatemeh ^a,b   Durocher, Yves ^a  

^a NATIONAL RESEARCH COUNCIL CANADA   (Canada)

^b MCGILL UNIVERSITY   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

ANIMAL CELL CULTURE; ANTIBODIES; CELLS; CHEMICAL REACTIONS; CHROMATOGRAPHIC ANALYSIS; CHROMATOGRAPHY; CLARIFICATION; CLONING; CYTOLOGY; DIFFUSERS (OPTICAL); DISSOCIATION; ELECTROPHORETIC MOBILITY; MAGNESIUM PRINTING PLATES; MAMMALS; MASS SPECTROMETRY; METRIC SYSTEM; PROTEIN FOLDING; PROTEINS; PURIFICATION; SIZE EXCLUSION CHROMATOGRAPHY;

CATION-EXCHANGE CHROMATOGRAPHY; CELL LINES; CYTOKINES; EFFICIENT METHODS; ELECTRON-TRANSFER DISSOCIATION; EXPRESSION SYSTEM; GLYCANS; GLYCOSIDASE; GLYCOSYLATED; HEK-293 CELLS; HIGH-LEVEL PRODUCTION; INTERMOLECULAR DISULFIDE BRIDGES; MAMMALIAN CELLS; MASS SPECTROSCOPY; N TERMINALS; NEURAMINIDASE; NEUTRAL PH; O-LINKED; POST-TRANSLATIONAL MODIFICATIONS; RECOMBINANT ANTIBODIES; RECOMBINANT PROTEINS; REPORTER GENES; SDS-PAGE; SINGLE PEAK; SINGLE-STEP; VOLUMETRIC PRODUCTION; VOLUMETRIC PRODUCTIVITY;

CELL CULTURE;

GLYCAN; GLYCOSIDASE; RECOMBINANT ALPHA2B INTERFERON; SIALIDASE; THREONINE; ALPHA2B INTERFERON; RECOMBINANT PROTEIN;

ACIDIFICATION; AMINO TERMINAL SEQUENCE; ANIMAL CELL; ANTIVIRAL ACTIVITY; ARTICLE; CELL CLONE; CELL CULTURE; CELL STRAIN HEK293; CONTROLLED STUDY; CULTURE MEDIUM; DISULFIDE BOND; DRUG PURIFICATION; DRUG SYNTHESIS; ELECTROPHORETIC MOBILITY; ELUTION; ENCEPHALOMYOCARDITIS VIRUS; GEL PERMEATION CHROMATOGRAPHY; HUMAN; HUMAN CELL; ION EXCHANGE CHROMATOGRAPHY; MASS SPECTROMETRY; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROCESS DEVELOPMENT; PRODUCT RECOVERY; PROTEIN GLYCOSYLATION; REPORTER GENE; VESICULAR STOMATITIS VIRUS; CELL LINE; GENETICS; KIDNEY; METABOLISM; METHODOLOGY; MOLECULAR CLONING; PHYSIOLOGY; PROTEIN ENGINEERING;

MAMMALIA;

CELL LINE; CLONING, MOLECULAR; HUMANS; INTERFERON ALFA-2B; KIDNEY; PROTEIN ENGINEERING; RECOMBINANT PROTEINS;

EID: 52249107853     PISSN: None     EISSN: 14726750     Source Type: Journal    
DOI: 10.1186/1472-6750-8-65     Document Type: Article

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