Simple and efficient control of CHO cell cultures

Journal of Biotechnology

Volumn 153, Issue 1-2, 2011, Pages 56-61

  Aehle, M ^a   Schaepe, S ^a   Kuprijanov, A ^a   Simutis, R ^b   Lubbert A ^a  

^a MARTIN LUTHER UNIVERSITY HALLE WITTENBERG   (Germany)

^b KAUNAS UNIVERSITY OF TECHNOLOGY   (Lithuania)

Author keywords

Closed loop control; Mammalian cell culture; Process reproducibility

Indexed keywords

AIR FLOW-RATE; CHO CELL; CLOSED-LOOP CONTROL; CULTIVATION TIME; EFFICIENT CONTROL; FED-BATCH CULTIVATION; FEED-RATES; MAMMALIAN CELL CULTURE; OFF-GAS ANALYSIS; PROCESS REPRODUCIBILITY; PRODUCT QUALITY; REPRODUCIBILITIES; SPECIFIC GROWTH RATE; STIRRED TANK BIOREACTORS; TOTAL MASS; VIABLE CELLS; VOLUME RATIO;

AMINO ACIDS; ANIMAL CELL CULTURE; CELLS; CLOSED LOOP CONTROL SYSTEMS; MAMMALS; OXYGEN;

CELL CULTURE;

GLUTAMINE; OXYGEN;

AIRFLOW; ANIMAL CELL; ARTICLE; CELL COUNT; CELL CULTURE; CELL GROWTH; CELL VIABILITY; CHO CELL; CONTROLLED STUDY; FED BATCH CULTURE; GAS ANALYSIS; GROWTH RATE; NONHUMAN; PRIORITY JOURNAL; REPRODUCIBILITY; STIRRED REACTOR;

ANIMALS; BIOREACTORS; CELL COUNT; CELL CULTURE TECHNIQUES; CELL SURVIVAL; CHO CELLS; CRICETINAE; CRICETULUS; FEEDBACK, PHYSIOLOGICAL; GLUTAMINE; OXYGEN; REFERENCE STANDARDS;

MAMMALIA;

EID: 79955545684     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2011.03.006     Document Type: Article

References (14)

1. Aehle M., Kuprijanov A., Schaepe S., Simutis R., Lübbert A. Increasing batch-to-batch reproducibility of CHO cultures by robust open-loop control. Cytotechnology 2011, 63:41-47.
2. Behrendt U., Koch S., Gooch D.D., Steegmans U., Comer M.J. Mass spectrometry: a tool for on-line monitoring of animal cell cultures. Cytotechnology 1994, 14:157-162.
3. Eyer K., Oeggerli A., Heinzle E. On-line gas analysis in animal cell cultivation: II. Methods for oxygen uptake rate estimation and its application to controlled feeding of glutamine. Biotechnol. Bioeng. 1994, 45:54-62.
4. Gnoth S., Jenzsch M., Simutis R., Lübbert A. Process analytical technology (PAT): batch-to-batch-reproducibility of fermentation processes by robust process operational design and control. J. Biotechnol. 2007, 132(2):180-186.
5. Jenzsch M., Gnoth S., Kleinschmidt M., Simutis R., Lübbert A. Improving the batch-to-batch reproducibility of microbial cultures during recombinant protein production by regulation of the total carbon dioxide production. J. Biotechnol. 2007, 128:858-867.
6. Lee Y.Y., Yap M.G.S., Hu W.-S., Wong K.T.K. Low-glutamine fed-batch cultures of H293-HEK serum-free suspension cells for adenovirus production. Biotechnol. Progr. 2003, 19:501-509.
7. Maranga L., Goochee C.F. Metabolism of PER.C6 cells cultivated under fed-batch conditions at low glucose and glutamine level. Biotechnol. Bioeng. 2006, 94(1):139-150.
8. Meneses A., Gomez A., Ramirez O.T. Animal cell technology: products from cells, cells as products. Proceedings of the 16th ESACT Meeting 1999, 23-29. Kluwer Academic Publishers, Dordrecht. A. Bernard, B. Griffiths, W. Noé, F. Wurm (Eds.).
9. Oeggerli A., Eyer K., Heinzle E. On-line gas analysis in animal cell cultivation: I. Control of dissolved oxygen and pH. Biotechnol. Bioeng. 1994, 45:42-53.
10. Pörtner R., Schwabe J.-O., Frahm B. Evaluation of selected control strategies for fed-batch cultures of a hybridoma cell line. Biotechnol. Appl. Biochem. 2004, 40:47-55.
11. Tremblay de M., Perrier M., Chavarie C., Archambault J. Fed-batch culture of hybridoma cells: comparison of optimal control. Bioprocess Eng. 1993, 9:13-21.
12. Sitton G., Srienc F. Mammalian cell culture scale-up and fed-batch control using automated flow cytometry. J. Biotechnol. 2008, 135:174-180.
13. Wlaschin K.F., Hu W.S. Fedbatch culture and dynamic nutrient feeding. Adv. Biochem. Eng. Biotechnol. 2006, 101:43-74.
14. Zhou W., Rehm J., Hu W.-S. High viable cell concentration fed-batch cultures of hybridoma cells through on-line nutrient feeding. Biotechnol. Bioeng. 1995, 46:579-587.