Identification of cell culture conditions to control N-glycosylation site-occupancy of recombinant glycoproteins expressed in CHO cells

Biotechnology and Bioengineering

Volumn 103, Issue 6, 2009, Pages 1164-1175

  Gawlitzek, Martin ^a   Estacio, Michael ^a   Fürch, Tobias ^a,b   Kiss, Robert ^a  

^a GENENTECH INC   (United States)

^b HELMHOLTZ CENTRE FOR INFECTION RESEARCH   (Germany)

Author keywords

Cell culture; CHO cells; Glycosylation; Sialic acid; Site occupancy

Indexed keywords

CHINESE HAMSTER OVARY CELLS; CHO CELL; CHO CELLS; CULTURE MEDIUM; ENVIRONMENTAL FACTORS; GUANOSINES; HUMAN TISSUES; MAMMALIAN CELL CULTURE; MEDIA COMPONENTS; N-GLYCAN; N-GLYCOSYLATION SITES; PRECURSOR MOLECULES; PRODUCT QUALITY; RECOMBINANT ENZYMES; RECOMBINANT GLYCOPROTEINS; SIALIC ACID; SITE-OCCUPANCY; SPECIFIC PRODUCTIVITY; THYROID HORMONES;

ACIDS; ANIMAL CELL CULTURE; CARBOXYLIC ACIDS; CELL MEMBRANES; ENZYMES; ESTERIFICATION; GLYCOPROTEINS; GLYCOSYLATION; HORMONES; IDENTIFICATION (CONTROL SYSTEMS); MAMMALS; MANGANESE; MOLECULES; QUALITY CONTROL; TIME MEASUREMENT;

CELL CULTURE;

ALTEPLASE; BUTYRIC ACID; GLYCOPROTEIN; GUANOSINE; IRON; LIOTHYRONINE; MANGANESE; MANNOSE; RECOMBINANT GLYCOPROTEIN 2; THYROID HORMONE; THYROXINE; UNCLASSIFIED DRUG; URIDINE;

ANIMAL CELL; ARTICLE; CELL CULTURE; CELL GROWTH; CONTROLLED STUDY; CULTURE MEDIUM; ENVIRONMENTAL FACTOR; GLYCOSYLATION; NONHUMAN; PH MEASUREMENT; PROTEIN EXPRESSION;

ANIMALS; CELL CULTURE TECHNIQUES; CHO CELLS; CRICETINAE; CRICETULUS; CULTURE MEDIA; GLYCOPROTEINS; GLYCOSYLATION; HUMANS; MEMBRANE GLYCOPROTEINS; RECOMBINANT PROTEINS; TEMPERATURE; TISSUE PLASMINOGEN ACTIVATOR;

CRICETULUS GRISEUS; MAMMALIA;

EID: 68149141804     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.22348     Document Type: Article

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