Metabolic signatures of GS-CHO cell clones associated with butyrate treatment and culture phase transition

Biotechnology and Bioengineering

Volumn 110, Issue 12, 2013, Pages 3244-3257

  Carinhas, Nuno ^a,b   Duarte, Tiago M ^a,b   Barreiro, Laura C ^a,b   Carrondo, Manuel J T ^a,b,c   Alves, Paula M ^a,b   Teixeira, Ana P ^a,b  

^a INSTITUTO DE BIOLOGIA EXPERIMENTAL E TECNOLÓGICA   (Portugal)

^b INSTITUTO DE TECNOLOGIA QUÍMICA E BIOLÓGICA   (Portugal)

^c UNIVERSIDADE NOVA DE LISBOA   (Portugal)

Author keywords

1H NMR; Butyrate treatment; Exometabolomics; GS CHO cell clones; Metabolic flux analysis; Specific productivity

Indexed keywords

1H-NMR; BUTYRATE TREATMENT; EXOMETABOLOMICS; GS-CHO CELLS; METABOLIC FLUX ANALYSIS; SPECIFIC PRODUCTIVITY;

AMINO ACIDS; CLONE CELLS; OPTIMIZATION; PHYSIOLOGY; VOLATILE FATTY ACIDS;

METABOLISM;

ACETIC ACID; ALANINE; AMMONIA; ARGININE; ASPARAGINE; ASPARTIC ACID; BUTYRIC ACID; CARBON; CHOLINE; CITRIC ACID; FORMIC ACID; FUMARIC ACID; GLUCOSE; GLUTAMATE AMMONIA LIGASE; GLUTAMIC ACID; GLUTAMINE; GLYCEROL; HISTIDINE; IMMUNOGLOBULIN G4; ISOBUTYRIC ACID; ISOLEUCINE; ISOVALERIC ACID; LACTIC ACID; LEUCINE; LYSINE; MALIC ACID; METHIONINE; PHOSPHORYLCHOLINE; SERINE; UNINDEXED DRUG;

ANIMAL CELL; ARTICLE; BIOPROCESS; CELL CULTURE; CELL FUNCTION; CELL METABOLISM; CHO CELL; CONTROLLED STUDY; DRY WEIGHT; ENZYME ANALYSIS; FEMALE; METABOLISM; METABOLOME; NITROGEN METABOLISM; NONHUMAN; PHASE TRANSITION; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTON NUCLEAR MAGNETIC RESONANCE; STABLE TRANSFECTION; WESTERN BLOTTING;

CRICETULUS GRISEUS;

1H-NMR; BUTYRATE TREATMENT; EXOMETABOLOMICS; GS-CHO CELL CLONES; METABOLIC FLUX ANALYSIS; SPECIFIC PRODUCTIVITY;

ANIMALS; BUTYRATES; CARBON; CHO CELLS; CRICETULUS; IMMUNOGLOBULIN G; NITROGEN; RECOMBINANT PROTEINS;

EID: 84886247487     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.24983     Document Type: Article

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