LC-MS-based metabolic characterization of high monoclonal antibody-producing Chinese hamster ovary cells

Biotechnology and Bioengineering

Volumn 109, Issue 12, 2012, Pages 3103-3111

  Chong, William Pooi Kat ^a   Thng, Shu Hui ^b   Hiu, Ai Ping ^a   Lee, Dong Yup ^a,b   Chan, Eric Chun Yong ^b   Ho, Ying Swan ^a  

^a AGENCY FOR SCIENCE   (Singapore)

^b NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

Author keywords

Chinese hamster ovary (CHO); High producer; Liquid chromatography mass spectrometry (LC MS); Metabolomics; Monoclonal antibody (mAb); Redox

Indexed keywords

CHINESE HAMSTER OVARY; HIGH PRODUCER; LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY; METABOLOMICS; MONOCLONAL ANTIBODIES (MAB); REDOX;

BATCH CELL CULTURE; CHROMATOGRAPHY; CITRIC ACID; CORRELATION METHODS; DISCRIMINANT ANALYSIS; METABOLISM; METABOLITES; MONOCLONAL ANTIBODIES; PEPTIDES; PHOSPHORYLATION; PRODUCTIVITY; RECOMBINANT PROTEINS; SUGARS;

BIOMOLECULES;

FLAVINE ADENINE NUCLEOTIDE; GLUTATHIONE; MONOCLONAL ANTIBODY; RECOMBINANT PROTEIN; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE;

ANIMAL CELL; ANTIBODY PRODUCTION; ARTICLE; CELL CLONE; CHO CELL; DISCRIMINANT ANALYSIS; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; METABOLOMICS; NONHUMAN; OXIDATION REDUCTION REACTION; PROTEIN PURIFICATION; SCORING SYSTEM; STATISTICAL ANALYSIS;

ANIMALS; ANTIBODIES, MONOCLONAL; BIOREACTORS; CHO CELLS; CHROMATOGRAPHY, LIQUID; CRICETINAE; CRICETULUS; GLUTATHIONE; MASS SPECTROMETRY; METABOLIC NETWORKS AND PATHWAYS; METABOLOME; METABOLOMICS; NUCLEOTIDES; PRINCIPAL COMPONENT ANALYSIS;

CRICETULUS GRISEUS; MAMMALIA;

EID: 84868010172     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.24580     Document Type: Article

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