Metabolite profiling of recombinant CHO cells: Designing tailored feeding regimes that enhance recombinant antibody production

Biotechnology and Bioengineering

Volumn 108, Issue 12, 2011, Pages 3025-3031

  Sellick, Christopher A ^a,d,e   Croxford, Alexandra S ^a   Maqsood, Arfa R ^a   Stephens, Gill ^b   Westerhoff, Hans V ^a,c   Goodacre, Royston ^a   Dickson, Alan J ^a  

^a UNIVERSITY OF MANCHESTER   (United Kingdom)

^b UNIVERSITY OF MANCHESTER   (United Kingdom)

^c UNIVERSITY OF AMSTERDAM   (Netherlands)

^d MEDIMMUNE   (United States)

^e UNIVERSITY OF NOTTINGHAM   (United Kingdom)

Author keywords

Antibody; Bioprocessing; CHO cells; GC MS; Glutamine synthetase; Metabolite profiling

Indexed keywords

BIOPROCESSING; CHO CELL; GC-MS; GLUTAMINE SYNTHETASE; METABOLITE PROFILING;

AMINO ACIDS; ANTIBODIES; BIOMOLECULES; CELLS; ENZYME ACTIVITY; METABOLISM; METABOLITES; NUTRIENTS; OPTIMIZATION;

CELL CULTURE;

IMMUNOGLOBULIN ANTIBODY; IMMUNOGLOBULIN G4; RECOMBINANT ANTIBODY;

ANIMAL CELL; ANTIBODY PRODUCTION; ANTIBODY TITER; ARTICLE; BIOMASS; CELL CULTURE; CHO CELL; CONTROLLED STUDY; METABOLITE; NONHUMAN; PROTEIN EXPRESSION;

ANIMALS; CELL CULTURE TECHNIQUES; CHO CELLS; CRICETINAE; CRICETULUS; CULTURE MEDIA; IMMUNOGLOBULIN G; METABOLOME; RECOMBINANT PROTEINS; TECHNOLOGY, PHARMACEUTICAL;

CRICETULUS GRISEUS;

EID: 81155160851     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.23269     Document Type: Article

References (13)

1. Baggetto LG, Clottes E, Vial C. 1992. Low mitochondrial proton leak due to high membrane cholesterol content and cytosolic creatine-kinase as 2 features of the deviant bioenergetics of Ehrlich and As30-D tumor-cells. Cancer Res 52(18): 4935-4941.
2. Castro PM, Hayter PM, Ison AP, Bull AT. 1992. Application of a statistical design to the optimization of culture medium for recombinant interferon-gamma production by Chinese hamster ovary cells. Appl Microbiol Biotechnol 38(1): 84-90.
3. Dorai H, Kyung YS, Ellis D, Kinney C, Lin C, Jan D, Moore G, Betenbaugh MJ. 2009. Expression of anti-apoptosis genes alters lactate metabolism of Chinese hamster ovary cells in culture. Biotechnol Bioeng 103(3): 592-608.
4. Kim SH, Lee GM. 2009. Development of serum-free medium supplemented with hydrolysates for the production of therapeutic antibodies in CHO cell cultures using design of experiments. Appl Microbiol Biotechnol 83(4): 639-648.
5. Lee GM, Kim EJ, Kim NS, Yoon SK, Ahn YH, Song JY. 1999. Development of a serum-free medium for the production of erythropoietin by suspension culture of recombinant Chinese hamster ovary cells using a statistical design. J Biotechnol 69(2-3): 85-93.
6. Ma N, Ellet J, Okediadi C, Hermes P, McCormick E, Casnocha S. 2009. A single nutrient feed supports both chemically defined NS0 and CHO fed-batch processes: Improved productivity and lactate metabolism. Biotechnol Prog 25(5): 1353-1363.
7. Rose D. 2009. Using metabolomics to optimize bioprocessing. Genet Eng Biotechnol News 29(15): 60-61.
8. Schiestl M, Stangler T, Torella C, Cepeljnik T, Toll H, Grau R. 2011. Acceptable changes in quality attributes of glycosylated biopharmaceuticals. Nat Biotechnol 29(4): 310-312.
9. Sellick CA, Hansen R, Maqsood AR, Dunn WB, Stephens GM, Goodacre R, Dickson AJ. 2009a. Effective quenching processes for physiologically valid metabolite profiling of suspension cultured mammalian cells. Anal Chem 81(1): 174-183.
10. Sellick CA, Maqsood AR, Croxford AS, Dickson AJ, Goodacre R, Stephens GM. 2009b. Optimizing CHO cell culture conditions. Genet Eng Biotechnol News 29(17): 56-57.
11. Sellick CA, Knight D, Croxford AS, Maqsood AR, Stephens GM, Goodacre R, Dickson AJ. 2010. Evaluation of extraction processes for intracellular metabolite profiling of mammalian cells-Matching extraction approaches to cell type and metabolite targets. Metabolomics 6(3): 427-438.
12. Sengupta N, Rose ST, Morgan JA. 2010. Metabolic flux analysis of CHO cell metabolism in the late non-growth phase. Biotechnol Bioeng 108(1): 82-92.
13. Yancey PH. 2005. Organic osmolytes as compatible, metabolic and counteracting cytoprotectants in high osmolarity and other stresses. J Exp Biol 208(15): 2819-2830.